Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.03423
Revista de Odontologia da UNESP
Original Article

A new proposal for evaluating of the solubility of bioceramic materials in dentin tubes after immersion in PBS: a laboratory investigation

Nova proposta para avaliação da solubilidade de materiais biocerâmicos em tubos de dentina após imersão em PBS: um estudo laboratorial

Giovanna da Cunha MENDONÇA; Karina Ines Medina Carita TAVARES; Airton Oliveira SANTOS-JUNIOR; Jáder Camilo PINTO; Juliane Maria GUERREIRO-TANOMARU; Mário TANOMARU-FILHO

http://dx.doi.org/10.1590/1807-2577.03423 Rev. odontol. UNESP, vol.52, e20230034, 2023
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Abstract

Introduction: Repair materials must have low solubility. Phosphate buffered saline (PBS) allows simulating clinical condition and interaction with dentin may be important for the correct evaluate of mass loss of bioceramic cements.

Objective: To evaluate the effect of distilled water (DW), or PBS immersion on the solubility of Bio-C Repair (BCR, Angelus) or MTA Repair HP (MTAHP, Angelus) using a dentin tube model.

Material and method: Bovine dentin tubes with a length of 4 mm, an internal diameter of 1.5 mm and walls thickness of approximately 1 mm were made. The specimens were immersed in DW for 24h, then filled with BCR or MTAHP (n = 14) and stored in an oven at 37°C and 95% humidity for 24h. After being weighed on a precision balance to determine the initial mass, the specimens were immersed in DW (pH 6.5) or PBS (pH 7.0) (n = 7) for 28 days. Empty tubes also were used for calculating the mass loss of the dentin (n=4). After this period, the specimens were weighed until stabilization of the final mass occurred (0.001g). The solubility of each material was evaluated. ANOVA and Tukey statistical tests were performed (α=0.05).

Result: BCR and MTAHP showing gain of mass in DW and mass loss in PBS (p<0.05).

Conclusion: The immersion solution influenced the solubility of BCR and MTAHP using dentin tube model. The new methodological proposal could be an alternative to ISO standards for testing the solubility of bioceramic cements.

Keywords

Calcarea silicata, endodontics, dental materials, physical properties

Resumo

Introdução: Materiais reparadores devem apresentar baixa solubilidade. Solução salina tamponada com fosfato (PBS) permite simular condição clínica e interação com dentina pode ser importante para correta avaliação da perda de massa de cimentos biocerâmicos.

Objetivo: Avaliou o efeito da imersão em água destilada (AD) ou PBS na solubilidade de Bio-C Repair (BCR, Angelus) ou MTA Repair HP (MTAHP, Angelus) usando modelo de tubo de dentina.

Material e método: Tubos de dentina bovina foram confeccionados com 4 mm de comprimento, 1,5 mm de diâmetro interno e 1 mm aproximadamente de espessura de parede. Os espécimes foram imersos em AD por 24h, posteriormente preenchidos com BCR ou MTAHP (n = 14) e armazenados em estufa a 37°C e umidade 95% por 24h. Após serem pesados em balança de precisão para determinação da massa inicial, os corpos de prova foram imersos em AD (pH 6,5) ou PBS (pH 7,0) (n = 7) por 28 dias. Tubos vazios também foram utilizados para o cálculo de perda de massa da dentina (n=4). Após esse período, os espécimes foram pesados até a estabilização da massa final (0,001g). A solubilidade de cada material foi avaliada. Testes estatísticos ANOVA e Tukey foram realizados (α=0,05).

Resultado: BCR e MTAHP apresentaram ganho de massa em AD e perda de massa em PBS (p<0,05).

Conclusão: A solução de imersão influencia a solubilidade de BCR e MTAHP usando modelo de tubo de dentina. Nova proposta metodológica poderá ser uma alternativa às normas ISO para testar a solubilidade de cimentos biocerâmicos.

Palavras-chave

Calcarea silicata, endodontia, materiais dentários, propriedades físicas

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Submitted date:
11/04/2023

Accepted date:
11/05/2023

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