VERIFICAÇÃO POR IMUNOFLUORESCÊNCIA INDIRETA DA PRESENÇA DE ANTICORPOS NO SORO E NA SALIVA DE HUMANOS DIRIGIDOS CONTRA Candida albicans
Verification by indirect immunofluorescence of the presence of antibodies in human serum and human saliva against Candida albicans
Cabral, L.A.G.
Rev. odontol. UNESP, vol.12, nÚnico, p.53-59, 1983
Resumo
Soro e saliva de 20 indivíduos sãos e de 20 pacientes com diagnósticos vários, que não "doença auto-imune", e apresentando sinais clínicos de candidíase bucal, foram investigados por imunofluorescência indireta (1.1.) quanto à presença de anticorpos (Acs) dirigidos contra Candida albicans. Os títulos séricos dos indivíduos normais variavam entre 4 e 64 e apenas um caso apresentou positividade de título 1 na saliva; nos pacientes com sinais clínicos de infecção bucal por Candida os títulos séricos, também todos positivos, variaram entre 32 e 2048 e na saliva a positividade foi constante com títulos de 1 a 16. Em um terceiro grupo, constituído de 30 pacientes com "doença auto-imune" e tratamento imunossupressor, os títulos séricos variavam de 32 a 2048 e os salivares de 1 a 8. Três pacientes com "doenças auto-imunes" sem o tratamento específico e sem sinais clínicos de infecção bucal por Candida tiveram os títulos séricos de 64, 8 e 8 e negatividade na saliva. A pesquisa pela 1.1. das classes G, M, A, D e E das imunoglobulinas,nas amostras de soro e saliva com concomitância de positividade de Acs contra C. albicans A, revelou a concorrência das classes IgG, IgM, IgA e IgD no soro e na saliva, quando de sinais clínicos de candidíase bucal.
Palavras-chave
Candidíases, imunossupressão, imunofluorescência, anticorpos séricos e salivares
Abstract
Serum and saliva of 20 healthy persons and of 20 patients with various diseases, not "autoimmune diseases", and showing buccal signals of candidiasis were investigated by indirect immunofluorescence (1.1.) as for the presence of antibodies (Abs) against Candida albicans A. The seric titles of healthy persons had a variation between 4 and 64 and only had presence of that Abs in saliva, with a title of 1; in the patients with buccal signals of candidiasis the seric titles, also positives, floated from 32 to 2048 and in saliva the positiveness was constant with titles of 1 to 16. ln a third group, constituted of 30 patients with "autommune diseases" and submitted to immunossupressive treatment, the seric titles were between 32 to 2048, while in saliva, 1 to 8. Three patients with "autoimmune diseases" without any treatment and presenting clinical signals of Candida infection, showed seric titles of 64, 8 and 8, and Abs were not detected in saliva. The ínvestigation by 1.1. of G,M,A,D, and E of the immunoglobulins classes, in the samples of serum and saliva, which presented concomitance of positivity of Abs against C.albicans A, showed the concurrence of the IgG, IgM, IgA and IgD in serum and saliva, in the presence of buccal signals of candidiasis.
Keywords
Candidiasis, immunossuppression, immunofluorescence seric and salivary antibodies
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