VERIFICAÇÃO POR IMUNOFLUORESCÊNCIA INDIRETA DA PRESENÇA DE ANTICORPOS NO SORO E NA SALIVA DE HUMANOS DIRIGIDOS CONTRA Candida albicans
Verification by indirect immunofluorescence of the presence of antibodies in human serum and human saliva against Candida albicans
Cabral, L.A.G.
Rev. odontol. UNESP, vol.12, nÚnico, p.53-59, 1983
Resumo
Soro e saliva de 20 indivíduos sãos e de 20 pacientes com diagnósticos vários, que não "doença auto-imune", e apresentando sinais clínicos de candidíase bucal, foram investigados por imunofluorescência indireta (1.1.) quanto à presença de anticorpos (Acs) dirigidos contra Candida albicans. Os títulos séricos dos indivíduos normais variavam entre 4 e 64 e apenas um caso apresentou positividade de título 1 na saliva; nos pacientes com sinais clínicos de infecção bucal por Candida os títulos séricos, também todos positivos, variaram entre 32 e 2048 e na saliva a positividade foi constante com títulos de 1 a 16. Em um terceiro grupo, constituído de 30 pacientes com "doença auto-imune" e tratamento imunossupressor, os títulos séricos variavam de 32 a 2048 e os salivares de 1 a 8. Três pacientes com "doenças auto-imunes" sem o tratamento específico e sem sinais clínicos de infecção bucal por Candida tiveram os títulos séricos de 64, 8 e 8 e negatividade na saliva. A pesquisa pela 1.1. das classes G, M, A, D e E das imunoglobulinas,nas amostras de soro e saliva com concomitância de positividade de Acs contra C. albicans A, revelou a concorrência das classes IgG, IgM, IgA e IgD no soro e na saliva, quando de sinais clínicos de candidíase bucal.
Palavras-chave
Candidíases, imunossupressão, imunofluorescência, anticorpos séricos e salivares
Abstract
Serum and saliva of 20 healthy persons and of 20 patients with various diseases, not "autoimmune diseases", and showing buccal signals of candidiasis were investigated by indirect immunofluorescence (1.1.) as for the presence of antibodies (Abs) against Candida albicans A. The seric titles of healthy persons had a variation between 4 and 64 and only had presence of that Abs in saliva, with a title of 1; in the patients with buccal signals of candidiasis the seric titles, also positives, floated from 32 to 2048 and in saliva the positiveness was constant with titles of 1 to 16. ln a third group, constituted of 30 patients with "autommune diseases" and submitted to immunossupressive treatment, the seric titles were between 32 to 2048, while in saliva, 1 to 8. Three patients with "autoimmune diseases" without any treatment and presenting clinical signals of Candida infection, showed seric titles of 64, 8 and 8, and Abs were not detected in saliva. The ínvestigation by 1.1. of G,M,A,D, and E of the immunoglobulins classes, in the samples of serum and saliva, which presented concomitance of positivity of Abs against C.albicans A, showed the concurrence of the IgG, IgM, IgA and IgD in serum and saliva, in the presence of buccal signals of candidiasis.
Keywords
Candidiasis, immunossuppression, immunofluorescence seric and salivary antibodies
References
1. BRANDTZAEG, P. - Human secretory immunoglobulins: II - salivary secretions from individuais with selectively excessively or defective synthesis of serum immunoglobulins. Clin. exp. ImmunoJ., 8:69-85, 1971.
2. BRANDTZAEG, P. - Mucosal and glandular distribution of immunoglobulin components: immunohistochemistry with a cold ethanol fixation technique. Immunology, 26: 1101-14, 1974.
3. BUDTZ-JORGENSEN, E. - Presisposing conditions for Candida - induced denture stomatitis. J. dento Res., 82:167-73, 1974.
4. BUDTZ-JORGENSEN, E. - Clinical aspects of Candida infection in denture weares. J. am. dento Ass., 69:474-9, 1978.
5. CALABRESI, P; EDWARDS, E.A. & SCHILLING, R.F. - Fluorescent antiglobulin studies in leukopenic and related disordes J. clin. Invest., 38:2091-9, 1959.
5. CLAMAN, N.H.; MERRIL, D.A. & HARTLEY, T.F. - Salivary immunoglobulins: normal adults values and dissociation between serum and salivary leveis. J. AlJerg., 40:151-9, 1967.
7. CRAIG, J.M. & FARBER, S. - The development of disseminated viceral mycosis during therapy for acute leukemia. Am. J. Pathol., 29:601, 1953.
8. DONLON, W.c. - Immunology in dentistry. J. amo dento Ass., 100:220-31, 1980.
9. ELLISON, S.A.; MASHIMO, P.A. & MANDEL, LD. - Immunochimical studies of human saliva: the demonstration of serum proteins in whole and parotida saliva. J. dento Res., 39:892-8. 1960.
10. FONSECA, J .B. - Candidíase: aspectos de interesse odontológico. ln: LACAZ, C.S. Candidíase. São Paulo - EPU - EDUSP, 1980. p. 130-46.
11. FRENKEL, J.K. - Role of conticosteroids as predisposing factors in fungai diseases. Lab. ln vest. , 11:1192-208, 1962.
12. FUNDENBERGER, H.H.; STITES, D.P.; CALDWELL, J.L. & WELLS, J.V. - Basic& clinical immunology. 2 ed., Los Altos, Lange, 1978. p. 310-2.
13. GOLDSTEIN, E.; GRICCO, M.H.; FINKEL, G. & LOURIA, D.B. - Studies on the pathogenesis of experimental Candida parapsilosis and Candida gui//iermondii infections in mice. J. infec. Dis., 115:293-302, 1965.
14. GREENBERGER, M.S. & COHEN, G. - Oral infection in immunossupressed renal transplant patients. Oral Surg., 43:875-85, 1977.
15. HASENCLEVER, H.F. & MITCHELL, W.O. - Antigenic studies of Candida: I - observation of two antigenic groups in Candida albicanso J. Bacteriol., 82:570-3, 1961.
16. HURLIMANN, J. - Immunoglobulin synthesis and transport by human salivary glands. Curr. topo Pathol., 55:69-108, 1971.
17. KEYES, J.D. & MAGEE, W.E. - Fungai diseases in general hospital; a study of 88 patients. Am. J. clin. Pathol., 26:1235-63, 1956.
18. KRAUS, F.W. & KONNO, J. - Antibodies in saliva. Ann. N. Y. Acad. Sei., 106: 311-29, 1963.
19. KRAUS, F. W. & KONNO, J. - The salivary secretion of antibody. Ala. J. medo Sei., 2: 15-21, 1965.
20. KRAUS, F.W. & SIRISINHA. S. - Gamma globulin in saliva. Arch. oral BioJ." 7: 221-33, 1962.
21. LEACH, L.B.; WYSHAK, G.K. & WEISBERGER, D. - Human saliva: its antigenic composition. l. dento Res., 42:568-74, 1963.
22. LEHNER. T. -Immunofluorescent investigation of Candida albicans antibodies in human saliva. Arch. oral. BioJ., 10: 975-80, 1965.
23. LEHNER, T. - Immunofluorescent study of Candida albicans in candidiasis, carriers and controls. l. Pathol. Bacteriol., 91: 97-104, 1966.
24. LEHNER, T. - Oral candidosis. Dent. practit., 17: 209-16, 1967.
25. LILIENTHALL, B. - Studies of the flora of the mouth: III - yeast - like organism; some observations on their incidence in the mouth. Aust. l. exp. Biol. medo Sci., 28: 279-86, 1950.
26. LOPEZ-MARTINEZ, R. - Características immunol6gicas en candidosis. Prensa medo Mex., 39: 468-71, 1974.
27. MARCUCCI, G. - Pênfigo foJiáceo: aspectos estomatológicos; estudo clínico, mico/ógico, citológico, histológico e histoquímico. São Paulo, Universidade de São Paulo. 1972. (Tese Doutoramento).
28. MOSKOW, B.S.; CRIKELAIR. G.F. & EHEATON, E.A. - Severe oral infection associated with prolonged steroid therapy, report of a case. Oral Surg., 34: 590-602, 1972.
29. NORRIS. R.F. & RAWSON, A.J. - Occurrence of serum agglutinins for Candida albicans and Saccharomyces cerevisiase in a hospital population. Am. l. clin. Pathol., 17: 813-9, 1947.
30. RIPPON. J.W. - Medical mycology. Philadelphia. Saunders. 1974. p. 175-204.
31. ROSEBURY, T. - Microorganisms indigenous to mano New York. MacGraw-Hill. 1962. p. 1-8.
32. SUMMERS, D.F.; GROLLMAN. A.P. & HASENCLEVER. H.F. - Polyssacharide antigens of Candida cell wall. l. /mmunol., 92: 491-9. 1964.
33. TERRANOVA, F.; GENOVESE. C. & CORDASCO. G. - Le classi immunoglobuliniche salivari in alcune affezione dei cavo orale. Minerva Stomatol., 25:9-11, 1976.
34. THONARD, J.C. - Host defense mechanism in the oral mucosa. Oral Surg., 42: 234-7, 1976.
35. TYLDESLEY. W.R.; ROTTER. E. & SELLS. R.A. - Oral lesions in renal transplant patients. l. oral. Pathol., 8: 53-9, 1979.
36. WILLIAMSON, J.J. - Diurnal variation of Candida albicans count in saliva. AUSI. dento l., 17: 54-60, 1972.
37. WILLIAMSON, J.J. - A study of extent of variation in daily counts of Candida albican,ç in saliva. Aust. dento l., 17: 106-9, 1972.
38. WINNER, H.I. - The epidemiology of candido-· sis./n: ALDOORY, Y. - Theepidemio/ogyof human mycotic diseases. Springfield, C.C. Thomas, 1975. p. 152-7.
39. WINNER. H.I. - A study of Candida albicans agglutinnins in human sera. l. Hyg., Cambridge, 53: 509-12, 1955.
40. WINNER, H.I. & HURLEY, R. - Candida albicans. London, Churchill, 1964. p. 44-52.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email