Análise Histológica dos Efeitos do Adesivo Dentinário Prime Bond 2.0 em Polpa de Ratos
Histologic analysis of the effects of an dentin adhesive prime bond 2.0 in pulp of rats
Minarelli Gaspar, A.M.; Balducci Roslindo, E.
Rev. odontol. UNESP, vol.32, n2, p.105-111, 2003
Resumo
O objetivo deste trabalho foi estudar os efeitos do adesivo dentinário Prime Bond 2.0 (Dentsply) em dentina e polpa de ratos por meio da análise ao microscópio de luz. A amostra consistiu de 20 ratos Rattus Norvegicus Holtzman, dos quais foram utilizados os 4 primeiros molares. O hemi-arco direito foi utilizado como grupo controle e os primeiro molares superiores e inferiores foram preenchidos com Hydro C (Dentsply) e recobertos com uma camada de resina restauradora Adaptic (Dentsply). Os molares do hemi-arco esquerdo, nos quais foi utilizado o adesivo dentinário Prime Bond 2.0 (Dentsply), foram restaurados com resina Adaptic (Dentsply) e os efeitos foram observados após 7, 15, 30 e 45 dias. O grupo tratado apresentou uma desorganização do tecido pulpar no período de 7 e 15 dias. Nos períodos seguintes, 30 e 45 dias, o processo inflamatório regrediu, a camada odontoblástica mostrou sinais de recuperação e observou-se a presença de dentina reacional. O grupo controle (Hydro C) não apresentou em nenhum período sinais de inflamação do tecido pulpar.
Palavras-chave
Adesivo dentinário, polpa, dentina, camada odontoblástica.
Abstract
The purpose of this work was to verify the effects of the Primer Bond 2.0 dentin adhesive in pulp and dentin of rats through the light microscopic. The sample consisted of 20 rats (Rattus Norvegicus Holtzman) whose first four molars were used. The right hemi-arch was used as a control group and the first superior and inferior molars had their cavities filled out with Hydro C (Dentsply) and recovered with a layer of restoring resin (Adaptic - Dentsply). In the molars of the left hemi-arch group treated, an acid attack was accomplished and later the cavities were covered with dentin adhesive Primer Bond 2.0 (Dentsply) and restored with Adaptic resin (Dentsply); the effects were observed after 7, 15, 30 and 45 days. The group being treated showed at the 7th and 15th days, a disorganization of the pulpar tissue with the presence of inflammatory cells and degeneration of the odontoblastic layer; in the subsequent periods, 30 and 45 days, the inflammatory process decreased, the odontoblastic layer showed signs of recovery and the presence of reactional dentin was observed. In the control group (Hydro C) signs of inflammatory process of the pulpar tissue were not present in any period
Keywords
Dentin adhesive, pulp, dentine, odontoblastic layer
References
1. BERGENHOLTZ, G. Effect of bacterial products on inflammatory reactions in the dental pulp. Scand. J. Dent. Res., Copenhagen, v. 85, n. 2, p. 122-129, Jan./Feb. 1977.
2. BUONOCORE, M.G. A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J. Dent. Res., Chicago, v. 34, n. 6, p. 849-853, Dec. 1955.
3. CAUSTON, B.E. Primers and mineralizing solutions. In: SMITH, D.C.; WILLKIMS, D.F. Biocompatibility of dental materials. Boca Raton: CRC Press, 1982. v. 2, p. 125-141 apud DEKON, A.F.C.; NAGEN FILHO, H; SOUZA, S.H.S. A inter-relação lama dentinária (“Smear layer”) com agentes de limpeza, condicionadores e “primers”. Rev. Fac. Odontol. Bauru, Bauru, v. 2, n. 3, p. 68-76, jul./set. 1994.
4. COSTA, C.A.S. et al. Compatibilidade biológica do ionômero de vidro fotopolimerizável (Variglass VLC). Avaliação histológica de seus efeitos sobre dentina e tecido pulpar em dentes de rato. Rev. Odontol. UNESP, São Paulo, v. 24, n. 2, p. 309-316, jul./dez. 1995.
5. COX, C.F. Biocompatibility of dental materials in the absence of bacterial infection. Oper. Dent., Seattle, v. 12, n. 4, p. 146-152, Autumn 1987.
6. COX, C.F. et al. Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primate teeth. Am. J. Dent., San Antonio, v. 11, sp. iss., p. 55-63, Jan. 1998.
7. ELBAUM, R.; PIGNOLY, C.; BROUILLET, J.L. A histology study of the biocompatibility of a dentinal bonding system. Quintessence Int., Berlin, v. 22, n. 11, p. 901-910, Nov. 1991.
8. ELBAUM, R.; REMUSAT, M.; BROUILLET, J.L. Biocompatibility of dentin and enamel adhesives. Quintessence Int., Berlin, v. 23, n. 11, p. 773-782, Nov. 1992.
9. FRANQUIN, J.C.; BROUILLET, J.L. Biocompatibility of an enamel and dentin adhesive under different conditions of application. Quintessence Int., Berlin, v. 19, n. 11, p. 813-826, Nov. 1988.
10. GORACCI, G.; MORI, G.; BAZZUCCHI, M. Marginal seal and biocompatibility of a fourth-generation bonding agent. Dent. Mater., Copenhagen, v. 11, n. 6, p. 343-347, Nov. 1995.
11. GRIEVE, A.R.; ALANI, A.; SAUNDERS, W.P. The effects on the dental pulp of a composite resin and two dentine bonding agents and associated bacterial microleakage. Int. Endod. J., Oxford, v. 24, n. 3, p. 108-118, May 1991.
12. HEBLING, J. Resposta do complexo dentino-pulpar à aplicação de um sistema adesivo em cavidades profundas com ou sem exposição da polpa. Avaliação histopatológica em dentes humanos. 1997. 193 f. Tese (Doutorado) - Faculdade de Odontologia, Universidade Estadual Paulista, Araraquara, 1997.
13. HEBLING, J.; GIRO, E.M.A.; COSTA, C.A.S. Human pulp response after an adhesive system application in deep cavities J. Dent., Chicago, v. 27, p. 557-564, Nov. 1999.
14. INOKOSHI, S.; IWAKU, M.; FUSAYAMA, T. Pulpal response to a new adhesive restorative resin. J. Dent. Res., Chicago, v. 61, p. 1014-1019, Aug. 1982.
15. JORDAN, R.E.; SUZUKI, M.; DAVIDSON, D. Clinical evaluation of a universal dentin bonding resin: preserving dentition through new materials. J. Am. Dent. Assoc., Chicago, v. 124, n. 11, p. 71-76, Nov. 1993.
16. MORSE, A. Formic acid sodium citrate descalcification and butyl alcohol deydratation of teeth and bones for sectioning in paraffin. J. Dent. Res., Chicago, v. 24, p. 143-153, 1945.
17. PAMEIJER, C.H.; STANLEY, H.R. Pulp reaction to a dentin bonding agent. Am. J. Dent., San Antonio, v. 8, n. 3, p. 140-144, June 1995.
18. PASHLEY, D.H. The effects of acid etching on the pulpodentin complex. Oper. Dent., Seattle, v. 17, n. 6, p. 229-242, Nov./Dec. 1992.
19. PERDIGAO, J.; FRANKENBERGER, R.; ROSA, B.T. New trends in dentin/enamel adhesion. Am. J. Dent., San Antonio, v. 13, spec. iss., p. 25D-30D, Nov. 2000.
20. RETIEF, D.H. et al. Phosphoric acid as a dentin etchant. Am. J. Dent., San Antonio, v. 5, n. 1, p. 24-28, Feb. 1992.
21. SMITH, D.C. General discussion. In: VANHERLE, G.; DEGRANGE, M.; WILLEMS, G. State of the art on direct posterior filling materials and dentin bonding. INTERNACIONAL SYMPOSIUM EURODISNEY, Paris,
1993. Proceedings... Paris, 1993. p. 251-269 apud TILTEY, K. et al. The composition and ultrastructure of resin tags in etched dentin. Am. J. Dent., San Antonio, v. 8, n. 5, p. 224-230, Oct. 1995.
22. SUGIZAKI, J. The effect of various primers on the dentin adhesion of resin composites. Jap. J. Conserv. Dent., Tokyo, v. 34, p. 228-265, 1991.
23. TEN CATE, A.R. Histologia bucal. Desenvolvimento, estrutura e função. 5. ed. Rio de Janeiro: Guanabara,
2001. p.143-185.
24. TITLEY, K. The composition and ultrastructure of resin tags in etched dentin. Am. J. Dent., San Antonio, v. 8, p. 224-230, Oct. 1995.
25. TORNEY, D.L. The retentive ability of acid-etched dentin. J. Prosthet. Dent., St. Louis, v. 39, n. 2, p. 169-172, Feb. 1978.
26. TORTENSON, B. Pulpal reaction to a dental adhesive in deep human cavities. Endod. Dent. Traumatol., Copenhagen, v.11, n. 4, p. 172-176, Aug. 1995.
27. VAN MEERBEEK, B. et al. Morphological aspects of resin-dentin interdiffusion zone observed with different dentin adhesive resins. J. Dent. Res., Chicago, v. 71, n. 8, p.1530-1544, Aug. 1992.
28. WHITE, K.C. et al. Pulpar response to adhesive resin systems applied on acid-etched vital dentin: damp versus dry primer application. Quintessence Int., Berlin, v. 25, n. 4, p. 259-268, Apr. 1994.
29. WHITE, K.C. et al. Histologic pulpal response of acid etching vital dentin. J. Dent. Res., Chicago, v. 71, sp. iss., p. 188, 1992. Abstract 658.
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