Estudo da respiração celular em germes dentários de ratos
Respiration cellular study of rat tooth genns
Cassoni, T.A.; Inaco, B.J.; Ramalho, L.T.O.; Utrilla, L.S.
Rev. odontol. UNESP, vol.20, nÚnico, p.63-66, 1991
Resumo
Foi estudada a respiração celular em germes dentários dos primeiros molares de ratos com idade variando entre 174 e 204 horas, antes de se completar o processo de calcificação. Utilizou-se o aparelho de Warburg para as medidas e verificou-se que o consumo de oxigênio teve início após 420 minutos, atingindo o nível máximo aos 720 minutos; após esse período os germes dentários apresentaram redução linear do consumo de 02.
Palavras-chave
Germe dentário, técnicas manométricas de Warburg
Abstract
We studied the celular respiration of the Wistar rats (174-204 hours old) first molar tooth germs, before mineralization by means of Warburg's technique for measurement. We verified the oxygen consumption was after 420 minutes, the maximum level 720 minutes, then the tooth germs showed linear reduction of oxygen consum.
Keywords
Tooth germ, Walburg manometric techniques
References
1. BERGQUIST, J.J., FISCHER, A.K. Endogenous oxigen consumption rates of bovine alveolar mucosa. J. dento Res., v. 49, p. 1522-6, 1970.
2. BOSIA, A., ARESE, P. Glycolytic enzyme activities in bovine hard dental tissue at different ages. Cale. Tiss. Res.• v. 6, p. 93-102, 1970.
3. FISCHER, A.K., CHWABE, C. Respiration and glycolysis in bovine dental pulp. J. dento Res., v. 48, p. 439-43, 1969.
4. FISCHER, A.K., Me KERCHER, T.C. The effect of eyanide on oxigen consumption in bovine dental pulp. J. dento Res., V. 48, p. 920-3, 1969.
5. FISCHER, A.K., SCHUMACHER, E.R., ROBINSON, N.P., SHARBONDY, G.P. Effects of dental drues and materiais on the rate of oxigen consumption in bovine dental pulp. J. dento Res., v. 36, p. 447-51, 1957.
6. FISCHER, A.K., BELDING, J.H., OPINSKY, J.S., SPINELLA, D.I. The influence of the stage of tooth development on the oxigen quotient of normal bovine dental pulp. J. dento Res., v. 38, p. 208-15, 1959.
7. FLIEDER, D.E., FISCHER, A.K. The rate endogenous oxigen eonsumption in bovine dental pulp. J. dento Res., v. 34, p. 921-6,1955.
8. GANGAROSA, L.P., ROSETT, T. Studies in the biochemistry of oral tissue: II. Respiration of various preparations of bovine attached gingiva. J. dento Res., v. 50, p. 163, 1971.
9. GLICKMAN, 1., TURESKY, S., MANHOLD, I.H. The oxigen consumption of healing gingiva. J. dento Res., v. 29, p. 429-35, 1955.
10. SASAKI, S. Studies on the respiration of the tooth germ. J. Bioehem., v. 46, p. 269-79, 1959.
11. UMBREIT, W.W., BURRIS, R.H., STAUFFER, I.F. Manometrie teeniques and tissue metabolismo 4 ed. Minneapolis: Burgess Publ. 1964. p. 61.