Revista de Odontologia da UNESP
https://revodontolunesp.com.br/journal/rou/article/doi/10.1590/1807-2577.1037
Revista de Odontologia da UNESP
Original Article

Efeitos da dentina sobre o pH e atividade antimicrobiana de diversas formulações com hidróxido de cálcio

Effects of dentin on the pH and antimicrobial activity of several calcium hydroxide formulations

Galoza, M. O. G.; Kuga, Milton Carlos; Silva, Daniele Fockink da; Weckwerth, Paulo Henrique; Keine, Kátia Cristina; Pereira, Jefferson Ricardo; Andrade, Marcelo Ferrarezi de

Downloads: 0
Views: 462

Resumo

Objetivo: Avaliar o efeito da dentina sobre o pH e a atividade antimicrobiana de formulações com hidróxido de cálcio. Material e método: O pH e a atividade antimicrobiana das formulações (G1- polietilenoglicol, G2- glicerina e paramonoclorofenol canforado ou G3- clorexidina) foram analisados nas formulações puras (G1A, G2A e G3A) ou acrescidas de dentina em pó (G1B, G2B e G3B). Tubos de polietileno foram preenchidos com uma das formulações e imersos em água destilada. O pH foi analisado após 24 horas, 7, 14 e 21 dias. A atividade antimicrobiana foi avaliada sobre Enterococcus faecalis (ATCC 29212), através do teste de difusão em ágar. Os resultados foram submetidos aos testes de ANOVA e Tukey (p=0,05). Resultado: A dentina não exerceu efeito sobre o pH das formulações, exceto ocasionando a redução em G1, no período de 24 horas (p<0,05). Nas demais formulações, não houve interferência da dentina sobre o pH (p>0,05). Quando comparados entre os grupos, em 24 horas e 7 dias, G1A e G1B apresentaram menor pH (p<0,05). Em 14 dias, G2A, G2B, G3A e G3B demonstraram similar pH (p>0,05), assim como entre G3B e G1A (p>0,05). Em 21 dias, todas as formulações demonstram semelhante pH (p>0,05). Os resultados em ordem decrescente da atividade antimicrobiana foram G2A=G2B>G3A=G3B>G1A=G1B (p<0,05). Conclusão: A dentina não interferiu no pH e na atividade antimicrobiana das diferentes formulações com hidróxido de cálcio, exceto na associação com o polietilenoglicol, em que ocasionou a redução do pH apenas no período de 24 horas. A formulação com paramonoclorofenol canforado apresentou maior atividade antimicrobiana, seguida da clorexidina e do polietilenoglicol.

Palavras-chave

Cálcio, endodontia, Enterococcus faecalis, pH.

Abstract

Objective: To evaluate the dentin effect on pH and antimicrobial activity of the calcium hydroxide chemical formulations. Material and method: pH and antimicrobial activity of calcium hydroxide formulations were evaluated (G1-polyethyleneglycol, G2-glycerin and camphorated paramonochlorophenol and G3-chlorhexidine). The formulations were evaluated alone (G1A, G2A e G3A) or with dentin powder (G1B, G2B and G3B). Polyethylene tubes were filled with the formulation and immersed in distilled water. After 24 hours, 7, 14 and 21 days, pH was evaluated. The antimicrobial activity was evaluated against Enterococcus faecalis by agar diffusion test. All data were submitted to ANOVA and Tukey tests (p=0.05). Result: None effect was observed on pH and antimicrobial activity by dentin on different chemical formulations, except the reduction occurred in G1 at 24 hours (p <0.05). When compared between the groups at 24 hours and 7 days, G1A and G1B had a lower pH (p <0.05). At 14 days, G2A, G2B, G3A and G3B showed similar pH (p <0.05) and between G3B and G1A (p> 0.05). At 21 days, all formulations were similar (p> 0.05). Regarding antimicrobial activity G2B G2A => = G3A G3B> = G1A G1B (p <0.05). Conclusion: The dentin did not affect the pH and antimicrobial activity of different formulations containing calcium hydroxide, except in association with polyethylene glycol, which caused the reduction of pH, within 24 hours. The formulation with paramonochlorophenol camphor showed higher antimicrobial activity, followed by chlorhexidine and polyethylene glycol.

Keywords

Calcium, endodontic, Enterococcus faecalis, pH.

References

1. Kawashima N, Wadachi R, Suda H, Yeng T, Parashos P. Root canal medicaments. Int Dent J. 2009 February;59(1):5-11. PMid:19323305.

2. Sathorn C, Parashos P, Messer H. Antibacterial efficacy of calcium hydroxide intracanal dressing: a systematic review and meta-analysis. Int Endod J. 2007 January;40(1):2-10. http://dx.doi.org/10.1111/j.1365-2591.2006.01197.x. PMid:17209826.

3. Mohammadi Z, Dummer PM. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J. 2011 August;44(8):697-730. http://dx.doi.org/10.1111/j.1365-2591.2011.01886.x. PMid:21535021.

4. Pacios MG, Silva C, López ME, Cecilia M. Antibacterial action of calcium hydroxide vehicles and calcium hydroxide pastes. J Investig Clin Dent. 2012 November;3(4):264-70. http://dx.doi.org/10.1111/j.2041-1626.2012.00147.x. PMid:23129141.

5. Murad C, Fariniuk LF, Fidel S, Fidel RAS, Sassone LM. Bacterial leakage in root canals filled with calcium hydroxide paste associated with different vehicles. Braz Dent J. 2008; 19(3):232-7. http://dx.doi.org/10.1590/S0103-64402008000300011. PMid:18949297.

6. Atila-Pektaş B, Yurdakul P, Gülmez D, Görduysus O. Antimicrobial effects of root canal medicaments against Enterococcus faecalis and Streptococcus mutans. Int Endod J. 2013 May;46(5):413-8. http://dx.doi.org/10.1111/iej.12004. PMid:23095092.

7. Sinha N, Patil S, Dodwad PK, Patil AC, Singh B. Evaluation of antimicrobial efficacy of calcium hydroxide paste, chlorhexidine gel, and a combination of both as intracanal medicament: An in vivo comparative study. J Conserv Dent. 2013 January;16(1):65-70. http://dx.doi.org/10.4103/0972-0707.105302. PMid:23349580.

8. De Faria-Júnior NB, Keine KC, Só MV, Weckwerth PH, Guerreiro-Tanomaru JM, Kuga MC. Residues of calcium hydroxide-based intracanal

medication associated with different vehicles: a scanning electron microscopy evaluation. Microsc Res Tech. 2012 July;75(7):898-902. http://dx.doi.org/10.1002/jemt.22010. PMid:22279037.

9. Pereira MS, Faria G, Bezerra da Silva LA, Tanomaru-Filho M, Kuga MC, Rossi MA. Response of mice connective tissue to intracanal dressings containing chlorhexidine. Microsc Res Tech. 2012 December;75(12):1653-8. http://dx.doi.org/10.1002/jemt.22112. PMid:22887775.

10. Rôças IN, Siqueira JF Jr. In vivo antimicrobial effects of endodontic treatment procedures as assessed by molecular microbiologic techniques. J Endod. 2011 March;37(3):304-10. http://dx.doi.org/10.1016/j.joen.2010.11.003. PMid:21329812.

11. Vianna ME, Zilio DM, Ferraz CC, Zaia AA, Souza-Filho FJ, Gomes BP. Concentration of hydrogen ions in several calcium hydroxide pastes over different periods of time. Braz Dent J. 2009; 20(5):382-8. http://dx.doi.org/10.1590/S0103-64402009000500005. PMid:20126906.

12. Estrela C, Sydney GB, Bammann LL, Felippe Júnior O. Mechanism of action of calcium and hydroxyl ions of calcium hydroxide on tissue and bacteria. Braz Dent J. 1995; 6(2):85-90. PMid:8688662.

13. Wang JD, Hume WR. Diffusion of hydrogen ion and hydroxyl ion from various sources through dentine. Int Endod J. 1988 January;21(1):17-26. http://dx.doi.org/10.1111/j.1365-2591.1988.tb00949.x. PMid:3273292.

14. Haapasalo M, Qian W, Portenier I, Waltimo T. Effects of dentin on the antimicrobial properties of endodontic medicaments. J Endod. 2007 August;33(8):917-25. http://dx.doi.org/10.1016/j.joen.2007.04.008. PMid:17878075.

15. Haapasalo HK, Sirén EK, Waltimo TM, Ørstavik D, Haapasalo MP. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J. 2000 March;33(2):126-31. http://dx.doi.org/10.1046/j.1365-2591.2000.00291.x. PMid:11307453.

16. Freire LG, Carvalho CN, Ferrari PH, Siqueira EL, Gavini G. Influence of dentin on pH of 2% chlorhexidine gel and calcium hydroxide alone or in combination. Dent Traumatol. 2010 June;26(3):276-80. http://dx.doi.org/10.1111/j.1600-9657.2010.00874.x. PMid:20456473.

17. Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res. 1987 August;66(8):1375-9. http://dx.doi.org/10.1177/00220345870660081801. PMid:3114347.

18. Kuga MC, Faria G, Weckwerth PH, Duarte MAH, Campos EA, Só MVR, et al. Evaluation of the pH, calcium release and antibacterial activity of MTA Fillapex. Rev Odontol UNESP. 2013 September-October;42(5):330-5. http://dx.doi.org/10.1590/S1807-25772013000500003.

19. Camps J, Pashley DH. Buffering action of human dentin in vitro. J Adhes Dent. 2000; 2(1):39-50. PMid:11317407.

20. Mori GG, Ferreira FC, Batista FR, Godoy AM, Nunes DC. Evaluation of the diffusion capacity of calcium hydroxide pastes through the dentinal tubules. Braz Oral Res. 2009 April-June;23(2):113-8. http://dx.doi.org/10.1590/S1806-83242009000200004. PMid:19684943.

21. Poorni S, Miglani R, Srinivasan MR, Indira R. Comparative evaluation of the surface tension and the pH of calcium hydroxide mixed with five different vehicles: an in vitro study. Indian J Dent Res. 2009 January-March;20(1):17-20. http://dx.doi.org/10.4103/0970-9290.49050. PMid:19336854.

22. Mohammadi Z, Abbott PV. Antimicrobial substantivity of root canal irrigants and medicaments: a review. Aust Endod J. 2009 December;35(3):131-9. http://dx.doi.org/10.1111/j.1747-4477.2009.00164.x. PMid:19961451.

23. Athanassiadis B, Abbott PV, George N, Walsh LJ. In vitro study of the inactivation by dentine of some endodontic medicaments and their bases. Aust Dent J. 2010 September;55(3):298-305. http://dx.doi.org/10.1111/j.1834-7819.2010.01238.x. PMid:20887518.

24. Vianna ME, Gomes BP, Sena NT, Zaia AA, Ferraz CC, Souza Filho FJ. In vitro evaluation of the susceptibility of endodontic pathogens to calcium hydroxide combined with different vehicles. Braz Dent J. 2005; 16(3):175-80. http://dx.doi.org/10.1590/S0103-64402005000300001. PMid:16429180.

25. Peters LB, Wesselink PR, Moorer WR. Penetration of bacteria in bovine root dentine in vitro. Int Endod J. 2000 January;33(1):28-36. http://dx.doi.org/10.1046/j.1365-2591.2000.00268.x. PMid:11307471.

26. Cwikla SJ, Bélanger M, Giguère S, Progulske-Fox A, Vertucci FJ. Dentinal tubule disinfection using three calcium hydroxide formulations. J Endod. 2005 January;31(1):50-2. http://dx.doi.org/10.1097/01.DON.0000134291.03828.D1. PMid:15614007.

588019d57f8c9d0a098b5370 rou Articles
Links & Downloads

Rev. odontol. UNESP

Share this page
Page Sections