Evaluation of the pH, calcium release and antibacterial activity of MTA Fillapex
Avaliação do pH, liberação de cálcio e atividade antibacteriana do MTA Fillapex
Kuga, Milton Carlos; Faria, Gisele; Weckwerth, Paulo Henrique; Duarte, M. A. H.; Campos, E. A.; Só, Marcos Vinicius Reis
Abstract
Objective: This study evaluated, in several analysis periods, pH and calcium release and antibacterial activity provided by MTA Fillapex sealer compared to Sealapex and AH Plus sealers. Material and method: Polyethylene tubes were filled with a sealer and immersed in distilled water. After 24 hours, 14 and 28 days, pH and calcium release by endodontic sealers were evaluated directly in water which the tubes were stored. Sealers antibacterial activity was evaluated against Enterococcus faecalis and Staphylococcus aureus by means of agar diffusion test. All data were submitted to ANOVA and Tukey tests (α=0.05). Result: In all periods evaluated, Sealapex had the highest pH value (p<0.05) in comparison to other sealers and MTA Fillapex provided higher pH values than AH Plus (p<0.05). In 14-days period, MTA Fillapex had greater calcium release value than Sealapex (p<0.05). In 28-days period, Sealapex provided higher calcium release than MTA Fillapex (p<0.05). In all periods, AH Plus provided lower calcium release than other sealers (p<0.05). In relation to E. faecalis, there were no differences among the sealers, in relation to antibacterial activity (p>0.05). In relation to S. aureus, Sealapex presented better antibacterial effectiveness than the MTA Fillapex and AH Plus (p<0.05), which were similar each other (p>0.05). Conclusion: In final evaluation period, pH values and calcium release provided by MTA Fillapex were lower than provided by Sealapex and higher than provided by AH Plus. The MTA Fillapex antimicrobial action was similar to other endodontic sealers.
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Resumo
Objetivo: O estudo avaliou, em diversos períodos de análise, o pH e liberação de cálcio e a atividade antibacteriana proporcionada pelo MTA Fillapex, em relação ao Sealapex e AH Plus. Material e método: Tubos de polietilenos foram preenchidos com um dos cimentos e imersos em água destilada. Após de 24 horas, 14 e 28 dias, o valor do pH e o cálcio liberado pelos cimentos foram avaliados diretamente na água destilada em que os espécimes permaneceram imersos. A atividade antibacteriana dos cimentos foi avaliada em culturas de Enterococcus faecalis ou Staphylococcus aureus, por meio do teste de difusão em ágar. Os dados obtidos foram submetidos aos testes de ANOVA e Tukey (α=0.05). Resultado: Em todos os períodos analizados, o Sealapex proporcionou os maiores valores de pH (p<0,05) em relação aos demais cimentos e o MTA Fillapex proporcionou maiores valores que o dos AH Plus (p<0,05). Em 14 dias, o MTA Fillapex promoveu maior liberação de cálcio que o proporcionado pelo Sealapex (p<0,05). Em 28 dias, o Sealapex proporcionou maior liberação de cálcio que o MTA Fillapex (p<0,05). Em todos os períodos, o AH Plus apresentou a menor liberação de cálcio em relação aos outros cimentos (p<0,05). Em relação ao E. faecalis, não foram observadas diferenças (p>0,05) entre as zonas de inibição de crescimento bacteriano produzidas pelos diveros cimentos. Em relação ao S. aureus, o Sealapex apresentou maior atividade antibacteriana que o MTA Fillapex e o AH Plus (p<0,05), que por sua vez foram semelhantes entre si (p>0,05). Conclusão: Após o período final de avaliação, o pH e a liberação de cálcio proporcionado pelo MTA Fillapex foram menores que os do Sealapex e maiores que os do AH Plus. A atividade antimicrobiana do MTA Fillapex não diferiu dos demais cimentos endodônticos.
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References
1. Parirokh M, Torabinejad M. Mineral trioxide aggregate comprehensive literature review-part III: clinical applications, drawbacks, and mechanism of action Endod. 2010; 36(3): 400‑13. PMid:20171353. http://dx.doi.org/10.1016/j.joen.2009.09.009
2. Silva EJ, Rosa TP, Herrera DR, Jacinto RC, Gomes BPFA, Zaia AA. Evaluation of cytotoxicity and physicochemical properties of calcium silicate-based endodontic sealer MTA Fillapex Endod. 2013; 39(2): 274-7. PMid:23321245. http://dx.doi.org/10.1016/j.joen.2012.06.030
3. Camilleri J, Gandolfi MG, Siboni F, Prati C. Dynamic sealing ability of MTA root canal sealer. Int Endod J. 2011; 44(1): 9-20. PMid:20646079. http://dx.doi.org/10.1111/j.1365-2591.2010.01774.x
4. Gomes-Filho JE, Watanabe S, Bernabé PF, de Moraes Costa MT mineral trioxide aggregate sealer stimulated mineralization Endod. 2009; 35(2): 256-60. PMid:19166785. http://dx.doi.org/10.1016/j.joen.2008.11.006
5. Huffman BP, Mai S, Pinna L, Weller RN, Primus CM, Gutmann JL, et al. Dislocation resistance of ProRoot Endo Sealer calcium silicate-based root canal sealer, from radicular dentine. Int Endod J. 2009; 42(1): 34-46. PMid:19125978. http://dx.doi.org/10.1111/j.1365-2591.2008.01490.x
6. Bin CV, Valera MC, Camargo SE, Rabelo SB, Silva GO, Balducci I, et al. Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate Endod. 2012; 38(4): 495-500. PMid:22414836. http://dx.doi.org/10.1016/j.joen.2011.11.003
7. Faria-Júnior NB, Tanomaru-Filho M, Berbert FL, Guerreiro-Tanomaru JM. Antibiofilm activity, pH and solubility of endodontic sealers. Int Endod J. 2013; 46(8): 755-62. PMid:23441819. http://dx.doi.org/10.1111/iej.12055
8. Holland R, de Souza V, Nery MJ, Faraco Júnior IM, Bernabé PF, Otoboni Filho JA, et al. Reaction of rat connective tissue to implanted dentin tube filed with mineral trioxide aggregate, Portland cement and calcium hydroxide. Braz Dent J. 2001; 12(1): 3-8. PMid:11210247.
9. Tanomaru-Filho M, Chaves Faleiros FB, Saçaki JN, Hungaro Duarte MA, Guerreiro-Tanomaru JM. Evaluation of pH and calcium ion release of root-end filling materials containing calcium hydroxide or mineral trioxide aggregate Endod. 2009; 35(10): 1418-21. PMid:19801243. http://dx.doi.org/10.1016/j.joen.2009.07.009
10. Gomes-Filho JE, Watanabe S, Lodi CS, Cintra LT, Nery MJ, Filho JA, et al. Rat tissue reaction to MTA Fillapex. Dent Traumatol. 2012; 28(6): 452-6. PMid:22151741. http://dx.doi.org/10.1111/j.1600-9657.2011.01096.x
11. Leonardo MR, da Silva LA, Tanomaru Filho M, Bonifácio KC, Ito IY. In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics Endod.2000; 26(7): 391-4. PMid:11199762. http://dx.doi.org/10.1097/00004770-200007000-00003
12. Vasconcelos BC, Bernardes RA, Cruz SM, Duarte MA, Padilha Pde M, Bernardineli N, et al. Evaluation of pH and calcium ion release of new root-end filling materials. Oral Surg Oral Med Oral Path Oral Radiol Endod 2009;108(1): 135-9. PMid:19451009. http://dx.doi.org/10.1016/j.tripleo.2009.02.026
13. Siqueira JF Jr, Favieri A, Gahyva SM, Moraes SR, Lima KC, Lopes HP. Antimicrobial activity and flow rate of newer and established root canal sealers Endod. 2000; 26(5): 274‑7. PMid:11199734. http://dx.doi.org/10.1097/00004770-200005000-00005
14. Silva LA, Leonardo MR, da Silva RS, Assed S, Guimaraes LF. Calcium hydroxide root canal sealers: evaluation of pH, calcium ion concentration and conductivity. Int Endod J. 1997; 30(3): 205-9. PMid:9477806. http://dx.doi.org/10.1111/j.1365-2591.1997.tb00698.x
15. Duarte MAH, Demarchi ACCO, Giaxa MH, Kuga MC, Fraga SC, Souza LCD. Evaluation of pH and calcium ion release of three root canal sealers Endod. 2000; 26(7): 389-90. PMid:11199761. http://dx.doi.org/10.1097/00004770-200007000-00002
16. Duarte MA, de Demarchi AC, de Moraes IG. Determination of pH and calcium ion release provided by pure and calcium hydroxide-containing AHPlus. Int Endod J. 2004; 37(1): 42-5. PMid:14718056. http://dx.doi.org/10.1111/j.1365-2591.2004.00756.x
17. Schäfer E, Zandbiglari T. Solubility of root-canal sealers in water and artificial saliva. Int Endod J. 2003; 36(10): 660-9. http://dx.doi.org/10.1046/j.1365-2591.2003.00705.x
18. Caicedo R, Von Fraunhofer JA. The properties of endodontic sealer cements Endod. 1988; 14(11): 527-34. http://dx.doi.org/10.1016/S0099-2399(88)80084-0
19. Borges RP, Sousa-Neto MD, Versiani MA, Rached-Júnior FA, De-Deus G, Miranda CE, et al. Changes in the surface of four calcium silicate-containing endodontic materials and an epoxy resin-based sealer afte solubility test. Int Endod J. 2012; 45(5): 419-28. PMid:22150403. http://dx.doi.org/10.1111/j.1365-2591.2011.01992.x
20. Keine KC, Guiotti FA, Leonardo RT, Kuga MC, Duarte MAH, Campos EA, et al. Influence of the addition of calcium hydroxide powder on some physical and chemical properties of the sealer MTA Fillapex. Worl Dent. 2012; 3(2): 190-3.
21. Gomes BP, Pedroso JA, Jacinto RC, Vianna ME, Ferraz CC, Zaia AA, et al. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J. 2004; 15(1): 30-5. PMid:15322642. http://dx.doi.org/10.1590/S0103-64402004000100006
22. Queiroz AM, Nelson-Filho P, Silva LAB, Assed S, Silva RAB, Ito IY. Antibacterial activity of root canal filling materials for primary teeth: zinc oxide and eugenol cement, Calen paste thickened with zinc oxide, Sealapex and EndoREZ. Braz Dent J. 2009; 20(4): 290-6. PMid:20069251. http://dx.doi.org/10.1590/S0103-64402009000400005
23. Çobankara FK, Altinöz HC, Erganis O, Kav K, Belli S. In vitro antibacterial activities of root-canal sealers by using two different methods Endod. 2004; 30(1): 57-60. PMid:14760911. http://dx.doi.org/10.1097/00004770-200401000-00013
24. Siqueira JF Jr. Aetiology of root canal treatment failure: why well treated teeth can fail. Int Endod J. 2001; 34(1): 1-10. http://dx.doi.org/10.1046/j.1365-2591.2001.00396.x
25. Morgental RD, Vier-Pelisser FV, Oliveira SD, Antunes FC, Cogo DM, Kopper PM. Antibacterial activity of two MTA-based root canal sealers. Int Endod J. 2011; 44(12): 1128-33 PMid:21895702. http://dx.doi.org/10.1111/j.1365-2591.2011.01931.x
26. Pumarola J, Berastegui E, Brau E, Canalda C, Jiménez de Anta MT. Antimicrobial activity of seven root canal sealers. Results of agar diffusion and agar dilution tests. Oral Surg Oral Med Oral Pathol. 1992; 74(2): 216-20. http://dx.doi.org/10.1016/0030-4220(92)90386-5
27. Leonardo MR, Bezerra da Silva LA, Tanomaru Filho M, Santana da Silva R. Release of formaldehyde b endodontic sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1999; 88(2): 221-5. http://dx.doi.org/10.1016/S1079-2104(99)70119-8
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