Microbiological evaluation of the effectiveness of an antibacterial toothbrush - in vivo study
Avaliação microbiológica da efetividade de uma escova antibacteriana: um estudo in vivo
Camargo, Roberta Araujo; Schimim, Sasha Cristina; Alves, Fabiana Bucholdz Teixeira; Chibinski, Ana Cláudia Rodrigues
http://dx.doi.org/10.1590/S1807-25772013000100010
Rev. odontol. UNESP, vol.42, n1, p.54-58, 2013
Abstract
This in vivo study aimed to evaluate the effectiveness of antibacterial toothbrushes compared to conventional toothbrushes in reducing microorganisms contamination. Methodology: Thirty children, 10 to 12 years-old, brushed their teeth with conventional and antibacterial toothbrushes two times a day without toothpaste, during two experimental periods of 5 days. The research was designed as a cross-over study with a wash‑out period of nine days between the different toothbrushes. After each trial period, the brushes were immediately immersed in individual flasks containing sterile saline solution. After agitation, the solution was seeded on plates containing the culture medium of Brain Heart Infusion, at concentrations of 10–2 e 10–3, to obtain total viable microorganisms. The plates were incubated for 24 hours at 37 °C under aerobiosis and colony-forming units were counted by means of a specific software. Result: In conventional toothbrushes group, median and interquartile values were 105 (27-564.5), while for the group of antibacterial toothbrushes were 53 (4.5-333.5). After analyzing the data, we observed no statistical differences between groups (p = 0.145). Conclusion: It was concluded that the antibacterial toothbrushes exhibited bacterial contamination similar to conventional toothbrushes.
Keywords
Toothbrushing, contamination, microbial viability.
Resumo
Este trabalho consistiu em um estudo in vivo para avaliar microbiologicamente a efetividade de uma escova antibacteriana comparada à de uma escova convencional. Metodologia: Trinta crianças de dez a 12 anos utilizaram escovas dentais convencionais e antibacterianas por dois períodos experimentais de cinco dias, com duas escovações diárias supervisionadas, sem dentifrício. O desenho do estudo foi cruzado com wash-out de nove dias entre as diferentes escovas. Ao término de cada período, as escovas foram imediatamente imersas em solução salina estéril e agitadas; o caldo produzido foi semeado em ágar BHI, nas concentrações de 10–² e 10–³. Após um período de incubação de 24 horas a 37 °C em aerobiose, foi realizada a contagem das unidades formadoras de colônias (UFC/mL) por meio de um software específico. Resultado: No grupo de escovas convencionais, a mediana e intervalos interquartis foi de 105 (27-564,5); já para o grupo de escovas antibacterianas, foi de 53 (4,5‑333,5). Após análise dos dados, observou-se ausência de diferenças estatísticas entre os grupos (p = 0,145). Conclusão: Concluiu‑se que as escovas de dentes antibacterianas exibiram contaminação bacteriana semelhante à das escovas de dentes convencionais.
Palavras-chave
Escovação dentária, contaminação, viabilidade microbiana.
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