Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.27915
Revista de Odontologia da UNESP
Original Article

Análise da eficácia antimicrobiana do ácido peracético na desinfecção de moldes de hidrocoloide irreversível

Analysis of the antimicrobial effectiveness of peracetic acid for the disinfection of irreversible hydrocolloid impressions

Moura, Carmem Dolores Vilarinho Soares de; Vasconcelos, Urias da Silva; Silva, Thalisson Saymo de Oliveira; Silva, Ângelo Antônio Rodrigues Irene da; Maranduba, Eduardo Crysnamuk Carvalho Saraiva Alves; Pacheco, Daylana da Silva; Mesquita, Avilnete Belém de Souza

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Resumo

Introdução: Nos últimos anos, o ácido peracético tem sido utilizado na Odontologia para desinfecção de moldes de hidrocoloide irreversível. Objetivo: Avaliar a eficácia antimicrobiana do ácido peracético nas técnicas de nebulização e pulverização, em moldes de hidrocoloide irreversível. Material e método: Oitenta moldes de hemiarcos de voluntários foram distribuídos aleatoriamente em quatro grupos experimentais (n=20), com respectivos controles. Os moldes foram submetidos à desinfecção por dez minutos com ácido peracético 0,2% e hipoclorito de sódio 1%, pelos métodos de nebulização, com atmosfera de 100% de umidade relativa, e pulverização. Após desinfecção, cada molde foi imerso em solução salina e submetido à vibração ultrassônica para dispersão de possíveis microrganismos. Para análise microbiológica desta solução, foi realizada contagem de colônias que cresceram em meio de cultura estéril BHI-ágar após 24 h em estufa incubadora a 37°C. Resultado: Em todos os grupos, foi significante a diferença no número médio de colônias decorrentes da análise estatística do controle e do grupo experimental correspondente (p<0,05). Comparando-se o ácido peracético (0,2%) nos dois métodos, não houve diferença significativa (p=0,420); no entanto, comparando-se o hipoclorito de sódio (1%) nos dois métodos, a diferença foi estatisticamente significante (p=0,010). No mesmo método e com soluções diferentes, a nebulização com ácido peracético 0,2% foi mais eficaz que o hipoclorito de sódio 1% (p=0,030). Conclusão: Ácido peracético 0,2% pode ser usado para desinfecção de moldes de hidrocoloide irreversível nos métodos de pulverização e nebulização. Hipoclorito de sódio 1% mostrou ser mais eficaz quando se usou o método de pulverização.

Palavras-chave

Desinfecção, materiais dentários, biossegurança, hipoclorito de sódio.

Abstract

Introduction: In recent years, peracetic acid has been used in dentistry for disinfection of irreversible hydrocolloid. Objective: To evaluate the antimicrobial effectiveness of peracetic acid using nebulization and spray techniques on irreversible hydrocolloid impressions. Material and method: 80 impressions of the hemi-arches of volunteers were randomly divided into four experimental groups (n = 20), with respective controls. The impressions were disinfected for 10 minutes with 0.2% peracetic acid and 1% sodium hypochlorite using the methods of nebulization in a 100% relative humidity atmosphere, and spraying. After disinfection, each impression was immersed in saline solution and subjected to ultrasonic vibration to disperse possible microorganisms. For the microbiological analysis of the solution, bacterial colonies, grown in sterile BHI-agar culture after 24h in an oven incubator at 37 ° C, were counted. Result: There was a statistically significant difference between the mean number of colonies of the control and each experimental group (p <0.05). There was no statistically significant difference between the two methods when using 0.2% peracetic acid (p = 0.420). However, there was a statistically significant difference between the two methods when using 1% sodium hypochlorite (p = 0.010). Nebulization with 0.2% peracetic acid was more effective than 1% sodium hypochlorite (p=0.030). Conclusion: 0.2% peracetic acid can be used for disinfection of irreversible hydrocolloid impressions using the methods of spraying or nebulization. Sodium hypochlorite (1%) proved to be more effective using the spraying method.

Keywords

Disinfection, dental materials, biosecurity, sodium hypochlorite.

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