Avaliação da combinação de poli  (ácido láctico-co-glicólico) e poli-isopreno (Cellprene®): estudo histológico em ratos

Isabella Fernanda dos SANTOS; Luis Alberto Loureiro dos SANTOS; Cassio Rocha SCARDUELI; Luis Carlos SPOLIDORIO; Elcio MARCANTONIO-JUNIOR; Camila Chiérici MARCANTONIO; Rosemary Adriana Chiérici MARCANTONIO

doi:10.1590/1807-2577.10819

Original Article

Avaliação da combinação de poli  (ácido láctico-co-glicólico) e poli-isopreno (Cellprene^®): estudo histológico em ratos

Evaluation of the combination of poly (Lactic-Co-Glycolic Acid) and polyisoprene (Cellprene^®) materials: histological study in rats

Isabella Fernanda dos SANTOS; Luis Alberto Loureiro dos SANTOS; Cassio Rocha SCARDUELI; Luis Carlos SPOLIDORIO; Elcio MARCANTONIO-JUNIOR; Camila Chiérici MARCANTONIO; Rosemary Adriana Chiérici MARCANTONIO

http://dx.doi.org/10.1590/1807-2577.10819 Rev. odontol. UNESP, vol.48, e20190108, 2019

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Resumo

Resumo: Introdução: A quantidade e qualidade óssea na implantodontia é um fator de alta relevância quando se tem por objetivo instalar implantes e reabilitar pacientes. No entanto, essa disponibilidade é comprometida na maioria dos casos, havendo a necessidade da busca de novos biomateriais, membranas e substâncias para uma regeneração mais favorável. Objetivo: O objetivo deste estudo foi avaliar a resposta da neoformação óssea em defeitos críticos em calvárias de ratos utilizando scaffolds de fibras de blenda polimérica a partir de poli (ácido láctico-co-glicólico) e poli-isopreno (Cellprene^®). O projeto foi aprovado pelo Comitê de Ética em Experimentação Animal.

Material e método: Neste estudo, foram utilizados 36 ratos (Rattus Norvegicus), variação albinus, Holtzman, adultos. Os animais foram submetidos à tricotomia na região da calota craniana e à confecção de defeitos ósseos circulares bilaterais com 5 mm de diâmetro. Os animais foram divididos em três grupos: GC – defeito sem colocação de biomaterial; GCol – scaffolds de colágeno (Bio-Gide, da empresa Geistlich Pharma Ag – Biomaterials); GPoli – scaffolds de fibras de blenda polimérica a partir de poli (ácido láctico-co-glicólico - Cellprene^®). Cada grupo foi avaliado em quatro períodos experimentais (7, 15, 30 e 60 dias). Após esses períodos, os animais foram sacrificados, e as peças passaram por tramitação laboratorial de rotina e inclusão em parafina. Foram obtidos cortes semisseriados e corados pela técnica de hematoxilina e eosina para análise histométrica e histológica. Foi executada análise histométrica para avaliar a composição do tecido ósseo reparado (% osso). Os dados obtidos foram analisados estatisticamente com nível de significância de 95%.

Resultado: Foi verificado que o GCol apresentou maior preenchimento do defeito nos períodos de 30 e 60 dias em comparação aos GC e GPoli.

Conclusão: Os scaffolds de fibras de blenda polimérica a partir de poli (ácido láctico-co-glicólico) e poli-isopreno (Cellprene^®) não apresentaram vantagens quando utilizados em defeitos críticos.

Palavras-chave

Histometria, regeneração óssea, biomaterial

Abstract

Abstract: Introduction: The bone quantity and quality in implant dentistry is a highly relevant factor when it aims the use of implants and rehabilitation in patients. However, this availability is compromised in most cases, with the need to research new biomaterials, membranes and substances for more favorable regeneration. Objective: The aim of this study was to evaluate the response of bone neoformation in critical defects in rat calvaries using polymeric blend fiber scaffolds from Poly (Lactic-Co-Glycolic Acid) and Polyisoprene (Cellprene^®). The project was approved by the Animal Experimentation Ethics Committee.

Material and method: In this study 36 rats (Rattus Norvegicus), variation albinus, Holtzman, adults were used. The animals had trichotomy in the region of the skull and the confection of bilateral circular bone defects with a diameter of 5 mm. The animals were divided into 3 groups: Group GC - defect without biomaterial placement, Group GCol - collagen scaffolds (Bio-Gide, from Geistlich Pharma Ag - Biomaterials), Group GPoli - polymeric blend fiber scaffolds from Poly (Lactic-Co-Glycolic Acid)-Polyisoprene. Each group was evaluated in 4 experimental periods (7, 15, 30 and 60 days). After these periods the animals were sacrificed and the pieces underwent routine laboratory procedures and paraffin embedding. Semi-serial sections were obtained and stained by hematoxylin and eosin technique for histometric and histological analysis. Histometric analysis was performed to evaluate the composition of repaired bone tissue (% Bone). The data obtained were statistically analyzed with a significance level of 95%.

Result: It was found that the GCol group presented greater defect filling in the 30 and 60 days periods compared to the GC and GPoli groups.

Conclusion: Polymer blend fiber scaffolds from Poly (Lactic-Co-Glycolic Acid) and Polyisoprene (Cellprene^®) did not have advantages when used in critical defects.

Keywords

Histometry, bone regeneration, biomaterial

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Avaliação da combinação de poli (ácido láctico-co-glicólico) e poli-isopreno (Cellprene®): estudo histológico em ratos

Evaluation of the combination of poly (Lactic-Co-Glycolic Acid) and polyisoprene (Cellprene®) materials: histological study in rats