Avaliação do efeito de cepas probióticas em biofilme de S. aureus sobre discos de titânio com superfície tratada

Mariana Cavalcante Costa MORAES; Pedro Jorge Cavalcante COSTA; Aguinaldo Silva Garcez SEGUNDO; Daiane Cristina PERUZZO

doi:10.1590/1807-2577.09619

Original Article

Avaliação do efeito de cepas probióticas em biofilme de S. aureus sobre discos de titânio com superfície tratada

Assessment of probiotic bacterial strains effect on S. aureus biofilm on titanium discs with treated surfaces

Mariana Cavalcante Costa MORAES; Pedro Jorge Cavalcante COSTA; Aguinaldo Silva Garcez SEGUNDO; Daiane Cristina PERUZZO

http://dx.doi.org/10.1590/1807-2577.09619 Rev. odontol. UNESP, vol.48, e20190096, 2019

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Resumo

Resumo: Introdução: As doenças peri-implantares apresentam como um dos principais fatores etiológicos o biofilme bacteriano, geralmente formado por uma microbiota semelhante à das doenças periodontais. Seu tratamento está centrado na descontaminação da superfície do implante e na remoção mecânica do biofilme, podendo ainda estar associado à administração de agentes antimicrobianos. Nesse sentido, tem sido cogitada a utilização de probióticos, que são microrganismos benéficos à saúde e que podem ter grande importância na cavidade oral, como coadjuvante no tratamento das peri-implantites.

Objetivo: Avaliar o efeito das cepas probióticas de Lactobacillus brevis e Bifidobacterium bifidum no crescimento do biofilme monoespécie de Staphylococcus aureus.

Material e método: Discos de titânio padronizados e com superfície tratada foram submersos em meio contendo caldo BHI e Staphylococcus aureus durante sete dias. Após esse período, o caldo foi retirado, os discos foram lavados e, então, introduzidos em um novo caldo BHI contendo as suspensões probióticas, sendo assim comparados a um grupo controle, sem probióticos. As amostras foram incubadas por 24h e então foram realizadas as diluições e a contagem das UFC (unidades formadoras de colônia) para Staphylococcus aureus.

Resultado: Após análise estatística dos dados, observou-se que a adição de ambos os probióticos resultaram em redução significativa (p<0,05) de UFC, quando comparados ao controle.

Conclusão: Conclui-se que os probióticos analisados (Lactobacillus brevis e Bifidobacterium bifidum) reduziram consideravelmente o crescimento do patógeno Staphylococcus aureus. Além disso, a cepa de Lactobacillus brevis apresentou efeito inibidor superior ao da cepa Bifidobacterium bifidum para ser utilizada como controle do biofilme bacteriano de Staphylococcus aureus.

Palavras-chave

Probióticos, peri-implantite, Bifidobacterium bifidum, Lactobacillus brevis

Abstract

Abstract: Introduction: One of the main etiological factors for peri-implant diseases is the bacterial biofilm, which usually features a similar microbiota to periodontal diseases. Its treatment focus on the decontamination of the implant surface and on the mechanical removal of biofilm, and it may also be associated to the administration of antimicrobial agents. Thus, the use of probiotics has been considered, since they feature beneficial microorganisms to health and may be of great importance for the oral cavity as an adjunct for the treatment of peri-implant diseases.

Objective: The aim of this in vitro study was to assess the effect of probiotic strains of Lactobacillus brevis and Bifidobacterium bifidum on the growth of single-species biofilm of Staphylococcus aureus.

Material and method: Standardized surface-treated titanium discs were submerged in a medium containing BHI broth and Staphylococcus aureus, for 7 days. After this period, the broth was removed, the discs were washed and, then, submerged in a new BHI broth containing probiotic suspensions and compared to a control group (with no probiotics). Samples were incubated for 24 hours and then the dilutions and CFU (colony-forming units) counting for Staphylococcus aureus were performed.

Result: Statistical analysis revealed that the addition of both probiotics resulted in a significant reduction (p<0,05) of CFU, when compared to the control group.

Conclusion: The assessed probiotics (Lactobacillus brevis and Bifidobacterium bifidum) considerably reduced Staphylococcus aureus growth. In addition, Lactobacillus brevis strain presented a superior inhibition effect than Bifidobacterium bifidum strain for Staphylococcus aureus bacterial biofilm control.

Keywords

Probiotics, peri- implantitis, Bifidobacterium bifidum, Lactobacillus brevis

References

Belibasakis GN. Microbiological and immuno-pathological aspects of peri-implant diseases. Arch Oral Biol. 2014 Jan;59(1):66-72. http://dx.doi.org/10.1016/j.archoralbio.2013.09.013. PMid:24209597.

Gualini F, Berglundh T. Immunohistochemical characteristics of inflammatory lesions at implants. J Clin Periodontol. 2003 Jan;30(1):14-8. http://dx.doi.org/10.1034/j.1600-051X.2003.300103.x. PMid:12702106.

Berglundh T, Gislason O, Lekholm U, Sennerby L, Lindhe J. Histopathological observations of human periimplantitis lesions. J Clin Periodontol. 2004 May;31(5):341-7. http://dx.doi.org/10.1111/j.1600-051X.2004.00486.x. PMid:15086615.

Mombelli A, Oosten MAC, Schürch E, Lang NP. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol Immunol. 1987 Dec;2(4):145-51. http://dx.doi.org/10.1111/j.1399-302X.1987.tb00298.x. PMid:3507627.

Mombelli A, Décaillet F. The characteristics of biofilms in peri-implant disease. J Clin Periodontol. 2011 Mar;38(Suppl 11):203-13. http://dx.doi.org/10.1111/j.1600-051X.2010.01666.x. PMid:21323716.

Persson GR, Samuelsson E, Lindahl C, Renvert S. Mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. II. Microbiological results. J Clin Periodontol. 2010 Jun;37(6):563-73. http://dx.doi.org/10.1111/j.1600-051X.2010.01561.x. PMid:20507380.

Persson GR, Renvert S. Cluster of bacteria associated with peri-implantitis. Clin Implant Dent Relat Res. 2014 Dec;16(6):783-93. http://dx.doi.org/10.1111/cid.12052. PMid:23527870.

Bastos EM, Brito F, Da Silva RM, Fischer RG, Figueredo CMS. Probióticos na terapia periodontal. Rev Bras Odontol. 2012 Jul-Dez;69(2):224-7.

Lahtinen SJ, Jalonen L, Ouwehand AC, Salminen SJ. Specific Bifidobacterium strains isolated from elderly subjects inhibit growth of Staphylococcus aureus. Int J Food Microbiol. 2007 Jun;117(1):125-8. http://dx.doi.org/10.1016/j.ijfoodmicro.2007.02.023. PMid:17462772.

Makino LES, Peralta FS, Scherma AP, Silva CRG, Leão MVP, Santos SSF. Avaliação in vitro da influência de lactobacillus casei na aderência de enterobacter cloacae em células epiteliais da mucosa jugal. Braz J Periodontol. 2014 Dec;24(4):15-21.

Riccia DN, Bizzini F, Perilli MG, Polimeni A, Trinchieri V, Amicosante G, et al. Anti-inflammatory effects of Lactobacillus brevis (CD2) on periodontal disease. Oral Dis. 2007 Jul;13(4):376-85. http://dx.doi.org/10.1111/j.1601-0825.2006.01291.x. PMid:17577323.

Guarner F, Perdigon G, Corthier G, Salminen S, Koletzko B, Morelli L. Should youghurt cultures be considered probiotic? Br J Nutr. 2005 Jun;93(6):783-6. http://dx.doi.org/10.1079/BJN20051428. PMid:16022746.

Thein ZN, Seneviratne CJ, Samaranayake YH, Samaranayake LP. Community lifestyle of Candidain in mixed biofilms: a mini review. Mycoses. 2009 Nov;52(6):467-75. http://dx.doi.org/10.1111/j.1439-0507.2009.01719.x. PMid:19486299.

Chioda TP, Schocken-Iturrino RP, Garcia GR, Pigatto CP, Ribeiro CAM, Ragazzani AVF. Inibição do crescimento de Escherichia coli isolada de Queijo “Minas Frescal” por Lactobacillus acidophilus. Cienc Rural. 2007 Mar- Abr;37(2):583-5. http://dx.doi.org/10.1590/S0103-84782007000200048.

Sung CE, Chiang CY, Chiu HC, Shieh YS, Lin FG, Fu E. Periodontal status of tooth adjacent to implant with peri-implantitis. J Dent. 2018 Mar;70:104-9. http://dx.doi.org/10.1016/j.jdent.2018.01.004. PMid:29326047.

Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infect. 2009 Jun;72(2):104-10. http://dx.doi.org/10.1016/j.jhin.2009.02.010. PMid:19329223.

Dias JCG, Santos CMML, Roxo MAP, Rocha LEMD, Terezan MLF. Influência clínica do uso de probióticos como adjuvantes no tratamento da periodontite crônica. Braz J Periodontol. 2018 Sep;28(3):36-45.

Lazarenko L, Babenko L, Sichel LS, Pidgorskyi V, Mokrozub V, Voronkova O, et al. Antagonistic action of lactobacilli and bifidobacteria in relation to Staphylococcus aureus and their influence on the immune response in cases of intravaginal staphylococcosis in mice. Probiotics Antimicrob Proteins. 2012 Jun;4(2):78-89. http://dx.doi.org/10.1007/s12602-012-9093-z. PMid:22611454.

Walencka E, Rózalska S, Sadowska B, Rózalska B. The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol (Praha). 2008;53(1):61-6. http://dx.doi.org/10.1007/s12223-008-0009-y. PMid:18481220.

Teanpaisan R, Piwat S. Lactobacillus paracasei SD1, a novel probiotic, reduces mutans streptococci in human volunteers: a randomized placebo-controlled trial. Clin Oral Investig. 2014 Apr;18(3):857-62. http://dx.doi.org/10.1007/s00784-013-1057-5. PMid:23892501.

Lee SH, Kim YJ. A comparative study of the effect of probiotics on cariogenic biofilm model for preventing dental caries. Arch Microbiol. 2014 Aug;196(8):601-9. http://dx.doi.org/10.1007/s00203-014-0998-7. PMid:24919536.

Teughels W, Loozen G, Quirynen M. Do probiotics offer opportunities to manipulate the periodontal oral microbiota? J Clin Periodontol. 2011 Mar;38(Suppl 11):159-77. http://dx.doi.org/10.1111/j.1600-051X.2010.01665.x. PMid:21323712.

Martin-Cabezas R, Davideau JL, Tenenbaum H, Huck O. Clinical efficacy of probiotics as an adjunctive therapy to non-surgical periodontal treatment of chronic periodontitis: a systematic review and meta-analysis. J Clin Periodontol. 2016 Jun;43(6):520-30. http://dx.doi.org/10.1111/jcpe.12545. PMid:26970230.