Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.09417
Revista de Odontologia da UNESP
Original Article

In vitro evaluation of the antibacterial behavior of a self-etch adhesive associated with chlorhexidine

Avaliação in vitro da ação anti-bacteriana de um adesivo auto-condicionante acrescido de clorexidina

Roberta Bento RODRIGUES; Nereida Mello GIOPPO; Priscilla do Monte Ribeiro BUSATO; Marcio José MENDONÇA; Veridiana CAMILOTTI

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Abstract

Abstract: Introduction: Adhesive technology has been developed quickly since its introduction about fifty years ago. Its goal is to produce a close contact within enamel and dentin. Failures in this process could result in microleakage, and allow the infiltration of bacteria, fluids and ions. Recent studies have been shown the use of chlorhexidine associated with adhesive systems can be able to inhibit the bacteria action.

Objective: The aim of this study was to evaluate the addiction of different percentages of chlorhexidine to a self-etch adhesive.

Methodology: The solution of digluconatechlorhexidine was increased to the primer of the two pass self etch adhesive to create a 0.5%, 1.0% and 2.0% chlorhexidine primer solution and after they were distributed in four groups (G1, G2, G3 and G4). Saliva samples (N=10) were used to test bacteria activity.

Result: They were spread in a blood medium with filter paper disks containing the different treatments. After the incubation, the inhibitions halos formation were evaluated.

Conclusion: This study demonstrated that, in vitro, the addition of different percentages of chlorhexidine digluconate to the self-etch adhesive induced inhibited halos at bacteria of saliva samples, independent from their concentration.

Keywords

Dentin adhesive, dentin, anti-bacterial agents

Resumo

Resumo: Introdução: A tecnologia adesiva vem se desenvolvendo rapidamente desde que foi introduzida há mais de 50 anos. O principal objetivo da adesão é alcançar um íntimo contato entre a estrutura dental e o material restaurador e fornecer adequada união entre o esmalte e a dentina.

Objetivo: A proposta deste estudo foi avaliar o efeito antibacteriano de um adesivo auto-condicionante de dois passos associado a diferentes concentrações de solução de digluconato de clorexidina (DCHX).

Metodologia: A solução de digluconato de clorexidina a 20% foi adicionada ao primer do sistema adesivo nas concentrações de 0,5, 1,0 e 2,0% e distribuídas em quatro grupos (G1, G2, G3 e G4). Para o teste de atividade antibacteriana foram utilizadas amostras de saliva (N=10) semeadas em meio de ágar sangue e discos de papel filtro contendo os diferentes grupos. Após incubação, as amostras foram inspecionadas quanto à formação de halo de inibição dos microrganismos através da utilização do paquímetro digital de precisão (0,0001mm) (Mitutoyou Sul Americana Ltda/ Starret Tools ®).

Resultado: Halos de inibição foram encontrados ao redor de todos os discos de filtro em que foram aplicados o primer acrescido de clorexidina mais o adesivo, bem como ao redor dos discos do controle positivo. Ao redor dos discos do controle negativo e dos discos com primer e adesivo não modificado não foram observados halos de inibição.

Conclusão: Este trabalho demonstrou que, in vitro, a adição de diferentes concentrações de digluconato de clorexidina ao sistema adesivo produziu halo de inibição dos microrganismos presentes nas amostras de saliva, independente da concentração utilizada.
 

Palavras-chave

Adesivo dentinário, dentina, agente antibacteriano

References

Masarwa N, Mohamed A, Abou-Rabii I, Abu Zaghlan R, Steier L. Longevity of self-etch dentin bonding adhesives compared to etch-and-rinse dentin bonding adhesives: a systematic review. J Evid Based Dent Pract. 2016 Jun;16(2):96-106. http://dx.doi.org/10.1016/j.jebdp.2016.03.003. PMid:27449836.

Giannini M, Makishi P, Ayres AP, Vermelho PM, Fronza BM, Nikaido T, et al. Self-etch adhesive systems: a literature review. Braz Dent J. 2015 Jan-Feb;26(1):3-10. http://dx.doi.org/10.1590/0103-6440201302442. PMid:25672377.

Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, et al. Buonocore memorial lecture. Adhesion to enamel and dentin: current status and future challenges. Oper Dent. 2003 May-Jun;28(3):215-35. PMid:12760693.

Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL. State of the art of self-etch adhesives. Dent Mater. 2011 Jan;27(1):17-28. http://dx.doi.org/10.1016/j.dental.2010.10.023. PMid:21109301.

Araújo MS, Souza LC, Apolonio FM, Barros LO, Reis A, Loguercio AD, et al. Two-year clinical evaluation of chlorhexidine incorporation in two-step self-etch adhesive. J Dent. 2015 Jan;43(1):140-8. http://dx.doi.org/10.1016/j.jdent.2014.07.010. PMid:25046536.

Nishitani Y, Hosaka K, Hoshika T, Yoshiyama M, Pashley DH. Effects of chlorhexidine in self-etching adhesive: 24 hours results. Dent Mater J. 2013;32(3):420-4. http://dx.doi.org/10.4012/dmj.2012-199. PMid:23719003.

Imazato S. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater. 2003 Sep;19(6):449-57. http://dx.doi.org/10.1016/S0109-5641(02)00102-1. PMid:12837391.

Imazato S, Ma S, Chen JH, Xu HH. Therapeutic polymers for dental adhesives: loading resins with bio-active components. Dent Mater. 2014 Jan;30(1):97-104. http://dx.doi.org/10.1016/j.dental.2013.06.003. PMid:23899387.

Hugo WB, Longworth AR. Some aspects of the mode of action of chlorhexidine. J Pharm Pharmacol. 1964 Oct;16(10):655-62. http://dx.doi.org/10.1111/j.2042-7158.1964.tb07384.x. PMid:14226440.

Inagaki LT, Dainezi VB, Alonso RC, Paula AB, Garcia-Godoy F, Puppin-Rontani RM, et al. Evaluation of sorption/solubility, softening, flexural strength and elastic modulus of experimental resin blends with chlorhexidine. J Dent. 2016 Jun;49:40-5. http://dx.doi.org/10.1016/j.jdent.2016.04.006. PMid:27101768.

Pupo YM, Farago PV, Nadal JM, Simão LC, Esmerino LA, Gomes OM, et al. Effect of a novel quaternary ammonium methacrylate polymer (QAMP) on adhesion and antibacterial properties of dental adhesives. Int J Mol Sci. 2014 May;15(5):8998-9015. http://dx.doi.org/10.3390/ijms15058998. PMid:24853131.

Jacobo C, Torrella F, Bravo-González LA, Ortiz AJ, Vicente A. In vitro study of the antibacterial properties and microbial colonization susceptibility of four self-etching adhesives used in orthodontics. Eur J Orthod. 2014 Apr;36(2):200-6. http://dx.doi.org/10.1093/ejo/cjt032. PMid:23720449.

Licio CB, Teixeira JC, Rocha MMNP, Jacques PB. Atividade antimicrobiana de sistemas adesivos contra Streptococcus mutans, Candida spp e Staphylococcus aureus. FOL. 2016 Jul-Dez;26(2):53-60.

Carrilho MR, Tay FR, Donnelly AM, Agee KA, Tjäderhane L, Mazzoni A, et al. Host-derived loss of dentin matrix stiffness associated with solubilization of collagen. J Biomed Mater Res B Appl Biomater. 2009 Jul;90B(1):373-80. http://dx.doi.org/10.1002/jbm.b.31295. PMid:19090493.

Greenstein G, Polson A. The role of local drug delivery in the management of periodontal diseases: a comprehensive review. J Periodontol. 1998 May;69(5):507-20. http://dx.doi.org/10.1902/jop.1998.69.5.507. PMid:9623893.

Ebrahimi Chaharom ME, Ajami AA, Abed Kahnamouei M, Jafari Navimipour E, Tehranchi P, Zand V, et al. Antibacterial effect of all-in-one self-etch adhesives on Enterococcus faecalis. J Dent Res Dent Clin Dent Prospects. 2014;8(4):225-9. http://dx.doi.org/10.5681/joddd.2014.040. PMid:25587384.

Penmetsa RK, Rekha A S, Poppuri KC, Prashanth P S, Garapati S. An in vitro evaluation of antibacterial properties of self etching dental adhesive systems. J Clin Diagn Res. 2014 Jul;8(7):ZC01-05. http://dx.doi.org/10.7860/JCDR/2014/9010.4467. PMid:25177626.

Li Y, Carrera C, Chen R, Li J, Lenton P, Rudney JD, et al. Degradation in the dentin-composite interface subjected to multi-species biofilm challenges. Acta Biomater. 2014 Jan;10(1):375-83. http://dx.doi.org/10.1016/j.actbio.2013.08.034. PMid:24008178.

Li F, Weir MD, Fouad AF, Xu HH. Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model. Dent Mater. 2014 Feb;30(2):182-91. http://dx.doi.org/10.1016/j.dental.2013.11.004. PMid:24332270.

Zhang K, Wang S, Zhou X, Xu HH, Weir MD, Ge Y, et al. Effect of antibacterial dental adhesive on multispecies biofilms formation. J Dent Res. 2015 Apr;94(4):622-9. http://dx.doi.org/10.1177/0022034515571416. PMid:25715378.

Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR. Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater. 2003 Jun;19(4):313-9. http://dx.doi.org/10.1016/S0109-5641(02)00060-X. PMid:12686296.

Cocco AR, Rosa WL, Silva AF, Lund RG, Piva E. A systematic review about antibacterial monomers used in dental adhesive systems: current status and further prospects. Dent Mater. 2015 Nov;31(11):1345-62. http://dx.doi.org/10.1016/j.dental.2015.08.155. PMid:26345999.

Breschi L, Cammelli F, Visintini E, Mazzoni A, Vita F, Carrilho M, et al. Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-month in vitro study. J Adhes Dent. 2009 Jun;11(3):191-8. PMid:19603582.

Shafiei F, Alikhani A, Alavi AA. Effect of chlorhexidine on bonding durability of two self-etching adhesives with and without antibacterial agent to dentin. Dent Res J (Isfahan). 2013 Nov;10(6):795-801. PMid:24379870.

Esteves CM, Ota-Tsuzuki C, Reis AF, Rodrigues JA. Antibacterial activity of various self-etching adhesive systems against oral streptococci. Oper Dent. 2010 Jul-Aug;35(4):448-53. http://dx.doi.org/10.2341/09-297-L. PMid:20672730.
 

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