Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.05422
Revista de Odontologia da UNESP
Original Article

Effect of toothpaste with high fluoride concentration on remineralization of dental substrate with artificial caries

Efeito de dentifrícios com alta concentração de flúor na remineralização de substrato dentário com cárie artificial

Diego Felipe Mardegan GONÇALVES; Érika Mayumi OMOTO; Yasmine Parischi MUSA ALI; André Luiz Fraga BRISO; Ticiane Cestari FAGUNDES

Downloads: 1
Views: 729

Abstract

Abstract: Introduction: fluoride toothpaste is widely used by the population.

Objective: dentifrices with a high concentration of sodium fluoride and the presence of tricalcium phosphate were developed, providing additional protection to patients vulnerable to the development of caries. This study aimed to evaluate the influence of different dentifrices on the surface and internal hardness and enamel wear after artificial caries, pH cycling, and toothbrushing.

Material and method: enamel blocks (4x4 mm) were obtained from 20 bovine teeth. The specimens were submitted to artificial caries lesion and then to pH cycling and brushing according to the dentifrice: Colgate Total 12 and Clinpro + Tricalcium Phosphate.

Result: after this period, they were submitted to analysis of superficial and internal hardness and profilometry. Scanning Electron Microscopy (SEM) was performed. The data obtained were evaluated by one-way Analysis of Variance (ANOVA) to evaluate the mineral recovery and profilometry, and two-way repeated measures ANOVA to evaluate the superficial and internal hardness.

Conclusion: analyzing the surface hardness, after caries induction, there was a significant reduction in hardness that was partially recovered regardless of the dentifrice used. For internal hardness, Clinpro obtained lower values on the control side and up to 90 micrometers on the test side. As for profilometry, Colgate Total 12 showed greater wear when compared to Clinpro. It was concluded that Clinpro promoted lower internal hardness, however, Colgate Total 12 resulted in greater surface wear of tooth enamel.

Keywords

Toothpaste, dental enamel, dental remineralization

Resumo

Resumo: Introdução: dentifrícios fluoretados são amplamente utilizados pela população. Foram desenvolvidos dentifrícios com alta concentração de fluoreto de sódio e presença de tricálcio fosfato, fornecendo proteção adicional a pacientes vulneráveis ao desenvolvimento da cárie.

Objetivo: o objetivo deste estudo foi avaliar a influência de diferentes dentifrícios na dureza superficial, interna e desgaste do esmalte após cárie artificial, ciclagem de pH e escovação.

Material e método: blocos de esmalte (4x4 mm) foram obtidos a partir de 20 dentes bovinos. Os espécimes foram submetidos à lesão de cárie artificial e, em seguida, à ciclagem de pH e escovação de acordo com o dentifrício: Colgate Total 12 e Clinpro + Tricálcio Fosfato. Após este período, foram submetidos à análise de dureza superficial e interna e perfilometria. Foi realizada microscopia eletrônica de varredura.

Resultado: os dados obtidos foram avaliados por ANOVA um critério para avaliar a recuperação mineral e perfilometria, ANOVA dois critérios medidas repetidas para avaliar a dureza superficial e interna.

Conclusão: analisando a dureza superficial, após a indução da cárie houve uma redução significativa na dureza que foi parcialmente recuperada independente do dentifrício utilizado. Para dureza interna, Clinpro obteve valores menores no lado controle e até 90 micrômetros no lado teste. Quanto à perfilometria, o Colgate Total 12 apresentou maior desgaste quando comparado ao Clinpro. Concluiu-se que Clinpro promoveu menor dureza interna, porém, o Colgate Total 12 resultou em maior desgaste superficial do esmalte dentário.
 

Palavras-chave

Creme dental, esmalte dental, remineralização dentária

References

1 Laurence B, Mould-Millman NK, Scannapieco FA, Abron A. Hospital admissions for pneumonia more likely with concomitant dental infections. Clin Oral Investig. 2015 Jul;19(6):1261-8. http://dx.doi.org/10.1007/s00784-014-1342-y. PMid:25359325.

2 Sanz M, Beighton D, Curtis MA, Cury JA, Dige I, Dommisch H, et al. Role of microbial biofilms in the maintenance of oral health and in the development of dental caries and periodontal diseases. Consensus report of group 1 of the Joint EFP/ORCA workshop on the boundaries between caries and periodontal disease. J Clin Periodontol. 2017 Mar;44(Suppl 18):S5-11. http://dx.doi.org/10.1111/jcpe.12682. PMid:28266109.

3 Velo MMAC, Magalhães AC, Shiota A, Farha ALH, Grizzo LT, Honório HM, et al. Profile of high-fluoride toothpastes combined or not with functionalized tri-calcium phosphate on root dentin caries control: an in vitro study. Am J Dent. 2018 Dec;31(6):290-6. PMid:30658374.

4 Cochrane NJ, Cai F, Huq NL, Burrow MF, Reynolds EC. New approaches to enhanced remineralization of tooth enamel. J Dent Res. 2010 Nov;89(11):1187-97. http://dx.doi.org/10.1177/0022034510376046. PMid:20739698.

5 Souza BM, Comar LP, Vertuan M, Fernandes C No, Buzalaf MA, Magalhães AC. Effect of an experimental paste with hydroxyapatite nanoparticles and fluoride on dental demineralisation and remineralisation in situ. Caries Res. 2015;49(5):499-507. http://dx.doi.org/10.1159/000438466. PMid:26278685.

6 Freitas MCCA, Nunes LV, Comar LP, Rios D, Magalhães AC, Honório HM, et al. In vitro effect of a resin infiltrant on different artificial caries-like enamel lesions. Arch Oral Biol. 2018 Nov;95:118-24. http://dx.doi.org/10.1016/j.archoralbio.2018.07.011. PMid:30099240.

7 Alania Y, Natale LC, Nesadal D, Vilela H, Magalhães AC, Braga RR. In vitro remineralization of artificial enamel caries with resin composites containing calcium phosphate particles. J Biomed Mater Res B Appl Biomater. 2019 Jul;107(5):1542-50. http://dx.doi.org/10.1002/jbm.b.34246. PMid:30296360.

8 Delbem AC, Danelon M, Sassaki KT, Vieira AE, Takeshita EM, Brighenti FL, et al. Effect of rinsing with water immediately after neutral gel and foam fluoride topical application on enamel remineralization: an in situ study. Arch Oral Biol. 2010 Nov;55(11):913-8. http://dx.doi.org/10.1016/j.archoralbio.2010.07.020. PMid:20813349.

9 Danelon M, Pessan JP, Souza F No, Camargo ER, Delbem AC. Effect of toothpaste with nano-sized trimetaphosphate on dental caries: in situ study. J Dent. 2015 Jul;43(7):806-13. http://dx.doi.org/10.1016/j.jdent.2015.04.010. PMid:25936338.

10 Feagin F, Koulourides T, Pigman W. The characterization of enamel surface demineralization, remineralization, and associated hardness changes in human and bovine material. Arch Oral Biol. 1969 Dec;14(12):1407-17. http://dx.doi.org/10.1016/0003-9969(69)90258-1. PMid:4903322.

11 Fernández CE, Tenuta LMA, Del Bel Cury AA, Nóbrega DF, Cury JA. Effect of 5,000 ppm fluoride dentifrice or 1,100 ppm fluoride dentifrice combined with acidulated phosphate fluoride on caries lesion inhibition and repair. Caries Res. 2017;51(3):179-87. http://dx.doi.org/10.1159/000453624. PMid:28222429.

12 Buzalaf MAR, Pessan JP, Honório HM, Cate JMT. Mechanisms of action of fluoride for caries control. Monogr Oral Sci. 2011;22:97-114. http://dx.doi.org/10.1159/000325151. PMid:21701194.

13 Walsh LJ. Contemporary technologies for remineralisation therapies: a review. Int Dent SA. 2009;11(6):6-16.

14 Karlinsey RL, Mackey AC, Walker ER, Frederick KE. Surfactant-modified beta-TCP: structure, properties, and in vitro remineralization of subsurface enamel lesions. J Mater Sci Mater Med. 2010 Jul;21(7):2009-20. http://dx.doi.org/10.1007/s10856-010-4064-y. PMid:20364363.

15 Karlinsey RL, Pfarrer AM. Fluoride plus functionalized β-TCP: a promising combination for robust remineralization. Adv Dent Res. 2012 Sep;24(2):48-52. http://dx.doi.org/10.1177/0022034512449463. PMid:22899679.

16 Karlinsey RL, Mackey AC. Solid-state preparation and dental application of an organically modified calcium phosphate. J Mater Sci. 2009;44(1):346-9. http://dx.doi.org/10.1007/s10853-008-3068-1.

17 Karlinsey RL, Mackey AC, Walker TJ, Frederick KE, Blanken DD, Flaig SM, et al. In vitro remineralization of human and bovine white-spot enamel lesions by NaF dentifrices: a pilot study. J Dent Oral Hyg. 2011 Feb;3(2):22-9. PMid:21643437.

18 Karlinsey RL, Mackey AC, Walker ER, Frederick KE. Preparation, characterization and in vitro efficacy of an acid-modified beta-TCP material for dental hard-tissue remineralization. Acta Biomater. 2010 Mar;6(3):969-78. http://dx.doi.org/10.1016/j.actbio.2009.08.034. PMid:19716443.

19 Duncan J, Macdonald JF, Hanna JV, Shirosaki Y, Hayakawa S, Osaka A, et al. The role of the chemical composition of monetite on the synthesis and properties of α-tricalcium phosphate. Mater Sci Eng C Mater Biol Appl. 2014 Jan;34:123-9. http://dx.doi.org/10.1016/j.msec.2013.08.038. PMid:24268241.

20 Cochrane NJ, Shen P, Yuan Y, Reynolds EC. Ion release from calcium and fluoride containing dental varnishes. Aust Dent J. 2014 Mar;59(1):100-5. http://dx.doi.org/10.1111/adj.12144. PMid:24494654.

21 Krämer N, Schmidt M, Lücker S, Domann E, Frankenberger R. Glass ionomer cement inhibits secondary caries in an in vitro biofilm model. Clin Oral Investig. 2018 Mar;22(2):1019-31. http://dx.doi.org/10.1007/s00784-017-2184-1. PMid:28741172.

22 Pini NI, Lima DA, Lovadino JR, Ganss C, Schlueter N. In vitro efficacy of experimental chitosan-containing solutions as anti-erosive agents in enamel. Caries Res. 2016;50(3):337-45. http://dx.doi.org/10.1159/000445758. PMid:27246229.

23 Nassar HM, Lippert F, Eckert GJ, Hara AT. Impact of toothbrushing frequency and toothpaste fluoride/abrasivity levels on incipient artificial caries lesion abrasion. J Dent. 2018 Sep;76:89-92. http://dx.doi.org/10.1016/j.jdent.2018.06.018. PMid:29940289.

24 Ganss C, Lussi A, Grunau O, Klimek J, Schlueter N. Conventional and anti-erosion fluoride toothpastes: effect on enamel erosion and erosion-abrasion. Caries Res. 2011;45(6):581-9. http://dx.doi.org/10.1159/000334318. PMid:22156703.

25 3M. Clinpro™ 5000. 1.1% sodium fluoride anti-cavity toothpaste. Technical product profile [Internet]. St. Paul: 3M; 2008 [cited 2023 Feb 9]. Available from: https://multimedia.3m.com/mws/media/544542O/3m-clinpro-5000-1-1-anti-cavity-toothpaste-technical-product-profile.pdf

26 Brown WE, Gregory TM, Chow LC. Effects of fluoride on enamel solubility and cariostasis. Caries Res. 1977;11(Suppl 1):118-41. http://dx.doi.org/10.1159/000260298. PMid:318567.
 


Submitted date:
12/20/2022

Accepted date:
02/09/2023

646cdc74a9539534232db053 rou Articles
Links & Downloads

Rev. odontol. UNESP

Share this page
Page Sections