Potencial antimicrobiano de diferentes retentores intrarradiculares frente a Enterococcus faecalis: uma avaliação in vitro

Nicole Hoffmann FINGER; Marília PAULUS; Alexandra Flávia GAZZONI

doi:10.1590/1807-2577.05119

Original Article

Potencial antimicrobiano de diferentes retentores intrarradiculares frente a Enterococcus faecalis: uma avaliação in vitro

Evaluation of antibacterial activity of different intraradicular retainers against Enterococcus faecalis: in vitro evaluation

Nicole Hoffmann FINGER; Marília PAULUS; Alexandra Flávia GAZZONI

http://dx.doi.org/10.1590/1807-2577.05119 Rev. odontol. UNESP, vol.48, e20190051, 2019

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Resumo

Resumo: Introdução: O tratamento endodôntico tem como objetivo erradicar microrganismos bacterianos e fúngicos do canal radicular, ou pelo menos possibilitar sua redução para níveis compatíveis com a saúde do tecido perirradicular, uma vez que, após o tratamento endodôntico, colônias de microrganismos residuais podem estar presentes nos túbulos dentinários e no cemento.

Objetivo: Avaliar o potencial antimicrobiano de diferentes tipos de retentores intrarradicularess em dentes bovinos.

Material e método: 50 dentes unirradiculares após tratamento endodôntico concluído foram desobturados, bem como tiveram seus condutos preparados para a cimentação dos retentores intrarradiculares. Posteriormente, os dentes foram contaminados por Enterococcus faecalis por meio da montagem de um dispositivo, o qual permitiu a infiltração bacteriana. Os dados resultantes da contagem das Unidades Formadoras de Colônia foram analisados utilizando o teste de ANOVA one-way, seguido pela análise de comparações múltiplas de Tukey.

Resultado: Decorrido o período de 14 dias, o grupo que apresentou a maior infiltração bacteriana foi o grupo-controle, o qual não continha nenhum tipo de retentor intrarradicular, seguido do G1-FV (grupo de continha retentor radicular de fibra de vidro) e G2-FC (grupo de que continha retentores radiculares de fibra de carbono). O menor grau de recuperação bacteriana foi encontrado nos grupos G3-Ni-Cr e G4-Cu-Al, os quais continham retentores intrarradiculares metálicos formados por ligas de níquel-cromo e cobre-alumínio, respectivamente.

Conclusão: Os retentores aqui avaliados apresentam diferentes efeitos antimicrobianos. Assim, a utilização de um pino com melhores resultados antimicrobianos favorece maiores chances de sucesso nos tratamentos reabilitadores.

Palavras-chave

Endodontia, técnica para retentor intrarradicular, infiltração dentária, obturação do canal radicular

Abstract

Abstract: Introduction: The ultimate goal of endodontic treatment is the eradication of microorganism from the root canal space, or at least their reduction to levels compatible with periradicular tissue health since after endodontic treatment colonies of residual microorganisms may be present in the dentinal tubules and cementum.

Objective: To evaluate the antibacterial activity of different fiber posts in the root canal system of bovine teeth.

Material and method: Here, 90 uniradicular bovine teeth were randomly assigned after complete endodontic therapy. The samples were unthreaded, as well as had their conduits prepared for the cementation of the intracanal retainers. Subsequently, the teeth were exposed to Enterococcus faecalis by means of the assembly of a device, which allowed bacterial infiltration. The data of the amount by colony forming unit (CFU) per ml assay were analyzed by one-way ANOVA followed by Tukey’s HSD post hoc test (p<0,05).

Result: After the 14-day period, the group that presented the highest bacterial infiltration was the control group, which did not contain any type of intrarradicular retainer, followed by G1-FV (group of contained fiber glass post) and G2- FC (group containing carbon fiber reinforced post). Results of the smallest degree of bacterial recovery was found in the G3-Ni-Cr and G4-Cu-Al groups, which contained metallic intracanal retainers by nickel-chromium and copper-aluminum alloys respectively.

Conclusion: The intracanal retainers evaluated here presented different antimicrobial effects. Thus, the use of intracanal post with better antimicrobial results, favors greater chances of success in rehabilitation treatments.

Keywords

Endodontics, post and core technique, dental leakage, root canal obturation

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