Revista de Odontologia da UNESP
Revista de Odontologia da UNESP
Original Article

Effect of pigmenting agents on tooth enamel staining during immediate tooth whitening: an in vitro study

Efeito de agentes pigmentantes no manchamento do esmalte dental durante o clareamento dentário imediato: estudo in vitro

Daniela Luzimar CLAUDINO; João Victor Frazão CÂMARA; Osmar de AGOSTINHO NETO; Ericles Otávio SANTOS; Gisele Damiana da Silveira PEREIRA; Isabel Ferreira BARBOSA

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Abstract: Introduction: Considering the increased search for esthetic procedures, questions emerge about the maintenance and regression of color, especially regarding the need to restrict dark foods during bleaching procedures or their influence on the stability and effectiveness of the treatment.

Objective: To assess the influence of staining agents on tooth enamel during immediate bleaching treatment with 35% hydrogen peroxide.

Material and method: Sixty bovine teeth were divided into six groups (G) (n=10): G1: distilled water (control); G2: coffee; G3: cola soft drink; G4: wine; G5: mate tea; and G6: industrialized açaí. The samples were subjected to immediate bleaching using 35% hydrogen peroxide for 21 days (three applications of 15 minutes per session, every seven days) and pigment immersion between each session for 15 minutes. The color was assessed before starting bleaching and after 21 days, using a spectrophotometer. The data obtained were subjected to one-way ANOVA and heteroscedasticity analysis by the Welch and Brown-Forsythe tests. The Tamhane test was used for group comparison.

Result: The color change was visible to the naked eye, considering the mean ΔE in all groups tested was higher than 3.7. However, there was no statistical difference between the control group and the groups assessed.

Conclusion: The contact of staining agents on bovine tooth enamel did not affect the final result of the immediate tooth bleaching.


Tooth bleaching, hydrogen peroxide, dyes, staining


Resumo: Introdução: Com o aumento da procura por procedimentos estéticos, dúvidas surgem quanto à manutenção e regressão de cor, especialmente, no que tange a necessidade de restrição quanto à alimentos escuros durante o procedimento clareador ou a sua influência quanto à estabilidade e eficácia do tratamento.

Objetivo: Avaliar a influência de agentes pigmentantes sobre o esmalte dental durante o tratamento clareador imediato com peróxido de hidrogênio a 35%.

Material e método: 60 dentes bovinos foram divididos em 6 grupos (G) (n=10): G1: água destilada (controle), G2: café, G3: refrigerante à base de cola, G4: vinho, G5: chá-mate, e G6: açaí industrializado. As amostras foram submetidas ao clareamento imediato utilizando peróxido de hidrogênio a 35% durante 21 dias (3 aplicações de 15 minutos por sessão a cada 7 dias) e submetidas à imersão dos pigmentos entre cada sessão durante 15 minutos. A avaliação da cor foi realizada antes do inicio do clareamento e após 21 dias, mediante o uso do espectrofotômetro. Os dados obtidos foram submetidos ao ANOVA one-way e análise de heterocedasticidade pelos testes de Welch e Brown-Forsythe. O teste de Tamhane foi utilizado para a comparação entre os grupos.

Resultado: Houve alteração de cor visível ao olho nu, uma vez que a média de ΔE em todos os grupos testados foi maior que 3,7. Contudo, não houve diferença estatística entre o grupo controle em relação aos grupos avaliados.

Conclusão: O contato de agentes pigmentantes no esmalte dental bovino não influenciou o resultado final do clareamento dental imediato.


Clareamento dental, peróxido de hidrogênio, corantes, pigmentação


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