Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.03524
Revista de Odontologia da UNESP
Original Article

ASU effect on bone repair in defects grafted with bone substitute

Efeito do ASU no reparo ósseo de defeitos enxertados com substituto ósseo

Lucas de Sousa Goulart PEREIRA; Luiz Guilherme Freitas de PAULA; Rubens SPIN-NETO; Andreas STAVROPOULOS; Rosemary Adriana Chiérici MARCANTONIO; Guilherme José Pimentel Lopes de OLIVEIRA

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Abstract

Introduction: Grafted areas represent a region of lower biological quality than areas of native bone. Bioactive products can improve bone quality in grafted areas.

Objective: To evaluate the effect of avocado/soybean unsaponifiables (ASU) on repair of critical-size calvarial defects (CSDs) filled with osteoconductive bone substitutes

Material and method: One CCD (0.5 mm) was made in each of 84 rats. These defects were filled either with coagulum (COA), deproteinized bovine bone (DBB), or tricalcium phosphate/hydroxyapatite (TCP/HA). ASU (0.6 g/kg) or saline solution (CTR) was administered daily by gavage from 15 days before surgery until the animals were euthanized (15 or 60 days after surgery) totaling 7 animals per period/group. The composition of the tissues that filled the CSDs were analyzed by histomorphometric evaluation, while the amount of mineralized tissue was evaluated by micro–CT.

Result: The bone filling in COA-ASU group was significantly higher than in the COA-CTR group (46.40 ±10.41% vs. 29.00 ± 8.81% at 15 days and 52.14 ± 6.12% vs. 42.71 ± 5.21% at 60 days) (p<0.05). There were no differences regarding the bone fill between the ASU and CTR groups in DBB and HA/TCP grafted CSDs. There were higher amount of mineralized tissues in the CSDs of the CTR groups than the ASU groups at 15 days (66.73 ± 6.70% vs. 52.25 ± 9.71% in DBB grafted CSDs and 53.16 ± 10.08% vs. 37.95 ± 4.70% in HA/TCP grafted CSDs) (p<0.05).

Conclusion: ASU enhanced the bone repair in the CSDs filled with COA; however, this positive effect was not seen in DBB or HA/TCP grafted CSDs.

 

Keywords

ASU; biomaterial; bone healing; critical-sized calvarial defects; histomorphometry; micro CT

Resumo

Introdução: Área enxertadas representam uma região de qualidade biológica inferior a áreas de osso nativo. Produtos bioativos podem melhorar a qualidade óssea em áreas enxertadas.

Objetivo: Avaliar o efeito do extrato de óleo insaponificável de abacate e soja (ASU) no reparo de defeitos críticos de calvaria (CSDs) preenchidos com substituto ósseo osteocondutor.

Material e método: Um defeito com 0,5 mm de diâmetro foi feito em cada um dos 84 ratos. Esses defeitos foram preenchidos com coágulo (COA), osso bovino desproteinizado (DBB) ou fosfato tricálcico/hidroxiapatita (HA/TCP). ASU (0,6 g/kg) ou solução salina (CTR) foi administrada diariamente por gavagem desde 15 dias antes da cirurgia até a eutanásia dos animais (15 ou 60 dias após a cirurgia) totalizando 7 animais por período/grupo. A composição dos tecidos que preencheram os DSCs foi analisada por avaliação histomorfométrica, enquanto que a quantidade de tecido mineralizado foi avaliada por micro-CT.

Resultado: O grupo preenchido por COA-ASU foi significativamente maior do que no grupo COA-CTR (46,40 ± 10,41% vs. 29,00 ± 8,81% aos 15 dias e 52,14 ± 6,12% vs. 42,71 ± 5,21% aos 60 dias) (p<0,05). Não houve diferenças quanto ao preenchimento ósseo entre os grupos ASU e CTR nos CSDs enxertados com DBB e HA/TCP. Houve maior quantidade de tecidos mineralizados nos CSDs dos grupos CTR do que nos grupos ASU aos 15 dias (66,73 ± 6,70% vs. 52,25 ± 9,71% nos CSDs enxertados com DBB e 53,16 ± 10,08% vs. 37,95 ± 4,70% nos CSDs enxertados com HA/TCP) (p<0,05).

Conclusão: ASU melhorou o reparo ósseo nos CSDs preenchidos com COA; no entanto, este efeito positivo não foi observado em CSDs enxertados com DBB ou HA/TCP.

Palavras-chave

ASU; biomateriais; reparo ósseo; defeitos críticos em calvaria; histomorfometria; micro-CT

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Submitted date:
10/21/2024

Accepted date:
10/22/2024

676477e9a953955970366d25 rou Articles
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