Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.03324
Revista de Odontologia da UNESP
Original Article

Efeito de uma membrana bioativa sobre a regeneração óssea guiada de defeitos críticos de calvária

Effect of a bioactive membrane on guided bone regeneration of critical calvary defects

Flavio Fidêncio de LIMA; Matheus UZAI; Natalia Nunes de SOUZA; Suzana Peres PIMENTEL; Fabiano Ribeiro CIRANO; Raissa Micaella Marcello MACHADO; Andrea Rodrigues Esposito CABRERA; Marcio Zaffalon CASATI; Mônica Grazieli CORRÊA

http://dx.doi.org/10.1590/1807-2577.03324 Rev. odontol. UNESP, vol.53, e20240033, 2024
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Resumo

Introdução: A regeneração óssea guiada (ROG) é uma abordagem que tem vindo a tornar-se cada vez mais conhecida por promover um tratamento regenerativo bem-sucedido. Esta técnica consiste na utilização de barreiras físicas para isolar a área a regenerar da influência não osteogénica das células do tecido conjuntivo gengival, protegendo o coágulo formado no espaço entre a barreira e o tecido ósseo preexistente.

Objetivo: Determinar o efeito da regeneração óssea guiada (ROG) por meio do uso de uma membrana com propriedade bioativa, composta por polímeros ácido polilático (PLA), policaprolactona (PCL) e polietileno glicol (PEG), associada à incorporação de bioativos sintéticos [beta-tricálcio fosfato (β-TCF), hidroxiapatita (HA) e nano-HA], no reparo ósseo em defeitos de calvária em ratos.

Material e método: Para isso, 21 ratos foram distribuídos nos grupos: Teste (n=7): Membrana Bioativa; Controle Positivo (n=7): Membrana BioGide®; Controle Negativo (n=7): sem biomaterial. Os animais foram submetidos à confecção de dois defeitos de calvária de tamanho crítico de 5mm de diâmetro. Quatro semanas após a confecção dos defeitos, ocorreu a eutanásia dos animais e as calvárias foram processadas para análise histológica e histomorfométrica.

Resultado: o grupo controle positivo (Bio-Gide) apresentou maior fechamento do defeito ósseo, em comparação ao grupo teste (Membrana Bioativa) e ao controle negativo (sem tratamento).

Conclusão: Dentro dos limites do presente estudo, pode-se concluir que o uso da Membrana com propriedade Bioativa não proporcionou regeneração óssea dos defeitos críticos em calvária de ratos.

Palavras-chave

Regeneração óssea guiada; membrana bioativa; defeito calvária

Abstract

Introduction: Guided bone regeneration (GBR) is an approach that has become increasingly well known for promoting successful regenerative treatment. This technique consists of using physical barriers to isolate the area to be regenerated from the non-osteogenic influence of gingival connective tissue cells, protecting the clot formed in the space between the barrier and the pre-existing bone tissue.

Objective: This study aimed at determining the effect of guided bone regeneration using a membrane with polylactic acid polymers by polylactic acid polymers), polycaprolactone (PCL) and polyethylenel (PEG) associated with the incorporation of bioactives [beta-tricalcium (β -TCF), hydroxyapatite (HA) and nano-HA], in the repair of calvaria repair in rats.

Material and method: For this, 21 rats were divided into groups: Test (=7): Bioactive Membrane; Positive Control (n=7): BioGide® Membrane; Negative Control (n=7): no treatment. Two critical defects were created in each animal at the start of the study (day 0). The animals were euthanized after 4 weeks of defects creation and the calvaria defects were processed for histological and histomorphometric analysis.

Result: The results indicate that the positive control group (Bio-Gide) presented grater closure of the bone defects compared to the test group (Bioactive Membrane) and the negative control (no treatment).

Conclusion: The use of the bioactive membrane did not promote bone generation (GBR) of calvaria defects in rats.

Keywords

Guided bone regeneration; bioactive membrane; calvaria defect

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Submitted date:
10/21/2024

Accepted date:
10/30/2024

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