Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.01123
Revista de Odontologia da UNESP
Original Article

Avaliação do comportamento das fibras colágenas periodontais durante a progressão da periodontite experimental em ratos

Evaluation of the behaviour of periodontal collagen fibers during the progression of experimental periodontitis in rats

Matheus Esnel GARCIA; Felipe de Lima FARIA; Bianca DAL OCA COSTA SILVA; Edilson ERVOLINO; Vivian Cristina Noronha NOVAES; Luan Felipe TORO

http://dx.doi.org/10.1590/1807-2577.01123 Rev. odontol. UNESP, vol.52, e20230011, 2023
PDF
Downloads: 0
Views: 869

Resumo

Introdução: A periodontite é uma doença infecto-inflamatória, resultante da disbiose microbiana e da resposta do hospedeiro, que leva à destruição dos tecidos de suporte dentário, inclusive das fibras colágenas periodontais, podendo culminar na perda do elemento dental.

Objetivo: Avaliar o comportamento das fibras colágenas periodontais durante a progressão da periodontite experimental induzida em ratos.

Material e método: Doze ratos Wistar foram distribuídos nos grupos: Controle (C), Periodontite Experimental 14-dias (PE-14d), Periodontite Experimental 21-dias (PE-21d) e Periodontite Experimental 42-dias (PE-42d). No dia 0, os animais do grupo C foram eutanasiados. Neste mesmo dia, os animais remanescentes foram submetidos à instalação de uma ligadura de algodão ao redor do primeiro molar inferior esquerdo para indução da periodontite experimental. Tais animais foram eutanasiados aos 14 (PE-14d), 21 (PE-21d) e 42 (PE-42d) dias após a instalação da ligadura. Executou-se o processamento histológico das hemimandíbulas e as secções foram submetidas à reação histoquímica pelo vermelho picro-sirius. A análise qualitativa descritiva foi realizada sob microscopia de luz polarizada, na região de furca dental, evidenciando as fibras do ligamento periodontal.

Resultado: O grupo C exibiu feixes espessos e orientados de fibras colágenas maduras, condizentes com aspecto de normalidade. Os grupos com periodontite experimental exibiram desestruturação tecidual severa, com fibras colágenas imaturas e de menor espessura, sendo tais condições mais exacerbadas nos grupos PE-14d e PE-21d.

Conclusão: As fases iniciais da periodontite apresentam caráter agudo e, portanto, resultam na rápida destruição dos tecidos periodontais de suporte, prejudicando potencialmente a fibrilogênese e a reestruturação do colágeno no ligamento periodontal.

Palavras-chave

Colágeno, periodontite, periodonto

Abstract

Abstract: Introduction: Periodontitis is an infectious-inflammatory disease resulting from microbial dysbiosis and host response that leads to the destruction of tooth support tissues, including periodontal collagen fibers, which may culminate in tooth loss.

Objective: To evaluate the behavior of periodontal collagen fibers during the progression of induced experimental periodontitis in rats.

Material and method: Twelve Wistar rats were distributed into groups: Control (C), 14-days Experimental Periodontitis (PE-14d), 21-days Experimental Periodontitis (PE-21d) and 42-days Experimental Periodontitis (PE-42d). At day 0, the animals of group C were euthanized. At the same day, the remaining animals were submitted to the installation of a cotton ligature around the lower left first molar for the induction of experimental periodontitis. The animals were euthanized at 14 (PE-14d), 21 (PE-21d) and 42 (PE-42d) days after the installation of ligature. Histological processing of the hemi-mandibles was performed and the sections underwent histochemical reaction using picro-sirius red. The descriptive qualitative analysis was performed under polarized light microscopy, in the dental furcation region, evidencing the fibers of the periodontal ligament.

Result: Group C exhibited thick and oriented bundles of mature collagen fibers, consistent with a normal appearance. The groups with experimental periodontitis exhibited severe tissue disruption, with immature and thinner collagen fibers, with such conditions being more exacerbated in the PE-14d and PE-21d groups.

Conclusion: The early stages of periodontitis present acute response, and therefore result in rapid destruction of periodontal support tissues and potentially impair fibrillogenesis and collagen restructuring in the periodontal ligament.
 

Keywords

Collagen, periodontitis, periodontium

References

1 Balic A. Biology explaining tooth repair and regeneration: a mini-review. Gerontology. 2018;64(4):382-8. http://dx.doi.org/10.1159/000486592. PMid:29533942.

2 Nanci A, Bosshardt DD. Structure of periodontal tissues in health and disease. Periodontol 2000. 2006;40(1):11-28. http://dx.doi.org/10.1111/j.1600-0757.2005.00141.x. PMid:16398683.

3 Bastos VC, Gomez RS, Gomes CC. Revisiting the human dental follicle: from tooth development to its association with unerupted or impacted teeth and pathological changes. Dev Dyn. 2022 Mar;251(3):408-23. http://dx.doi.org/10.1002/dvdy.406. PMid:34324745.

4 Cho MI, Garant PR. Development and general structure of the periodontium. Periodontol 2000. 2000 Oct;24(1):9-27. http://dx.doi.org/10.1034/j.1600-0757.2000.2240102.x. PMid:11276876.

5 Hassell TM. Tissues and cells of the periodontium. Periodontol 2000. 1993 Oct;3(1):9-38. http://dx.doi.org/10.1111/j.1600-0757.1993.tb00230.x. PMid:9673156.

6 Jong T, Bakker AD, Everts V, Smit TH. The intricate anatomy of the periodontal ligament and its development: lessons for periodontal regeneration. J Periodontal Res. 2017 Dec;52(6):965-74. http://dx.doi.org/10.1111/jre.12477. PMid:28635007.

7 Van Dyke TE, Sima C. Understanding resolution of inflammation in periodontal diseases: is chronic inflammatory periodontitis a failure to resolve? Periodontol 2000. 2020 Feb;82(1):205-13. http://dx.doi.org/10.1111/prd.12317. PMid:31850636.

8 Armitage GC. Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000. 2004;34(1):9-21. http://dx.doi.org/10.1046/j.0906-6713.2002.003421.x. PMid:14717852.

9 Cekici A, Kantarci A, Hasturk H, Van Dyke TE. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000. 2014 Feb;64(1):57-80. http://dx.doi.org/10.1111/prd.12002. PMid:24320956.

10 Ramseier CA, Anerud A, Dulac M, Lulic M, Cullinan MP, Seymour GJ, et al. Natural history of periodontitis: disease progression and tooth loss over 40 years. J Clin Periodontol. 2017 Dec;44(12):1182-91. http://dx.doi.org/10.1111/jcpe.12782. PMid:28733997.

11 Page RC, Schroeder HE. Pathogenesis of inflammatory periodontal disease. A summary of current work. Lab Invest. 1976 Mar;34(3):235-49. PMid:765622.

12 Toro LF, Mello-Neto JM, Issa JPM, Gonçalves A, Theodoro LH, Garcia VG, et al. Resposta imune-inflamatória durante a progressão da periodontite experimental ao longo do tratamento com zoledronato. Braz Oral Res. 2017;31(Suppl 2):386.

13 Statkievicz C, Toro LF, Mello-Neto JM, de Sá DP, Casatti CA, Issa JPM, et al. Photomodulation multiple sessions as a promising preventive therapy for medication-related osteonecrosis of the jaws after tooth extraction in rats. J Photochem Photobiol B. 2018 Jul;184:7-17. http://dx.doi.org/10.1016/j.jphotobiol.2018.05.004. PMid:29777942.

14 Silva GCB, Melo OM No, Nascimento AMV, Santos CAO, Nóbrega WFS, Souza SLX. Natural history of periodontal disease: a systematic review. Res Soc Dev. 2020;9(7):e607974562. http://dx.doi.org/10.33448/rsd-v9i7.4562.

15 Almeida RF, Pinho MM, Lima C, Faria I, Santos P, Bordalo C. Associação entre doença periodontal e patologias sistêmicas. Rev Port Med Geral Fam. 2006;22(3):379-90.

16 Steffens JP, Marcantonio RAC. Classificação das doenças e condições peri-implantares 2018: guia prático e pontos-chave. Rev Odontol UNESP. 2018;47(4):189-97. http://dx.doi.org/10.1590/1807-2577.04704.

17 Marcondes MS, Gomes FC, Anbinder AL. Influência do tratamento com sinvastatina na periodontite induzida em ratos. Rev Bras Odontol. 2008;65(1):122-5.

18 Sun J, Eberhard J, Glage S, Held N, Voigt H, Schwabe K, et al. Development of a peri-implantitis model in the rat. Clin Oral Implants Res. 2020 Mar;31(3):203-14. http://dx.doi.org/10.1111/clr.13556. PMid:31701561.

19 Ganganna K, Shetty P, Shroff SE. Collagen in histologic stages of oral submucous fibrosis: a polarizing microscopic study. J Oral Maxillofac Pathol. 2012 May;16(2):162-6. http://dx.doi.org/10.4103/0973-029X.98446. PMid:22923884.

20 Lima HG, Lara VS. Aspectos imunológicos da doença periodontal inflamatória: participação dos mastócitos. UNOPAR Cient Ciênc Biol Saúde. 2013;15(3):225-9.

21 Geraldo JM, Alfenas RC. Papel da dieta na prevenção e no controle da inflamação crônica: evidências atuais. Arq Bras Endocrinol Metabol. 2008;52(6):951-67. http://dx.doi.org/10.1590/S0004-27302008000600006. PMid:18820806.

22 Yared KFG, Zenobio EG, Pacheco W. A etiologia multifatorial da recessão periodontal. Rev Dent Press Ortodon Ortop Facial. 2006;11(6):45-51. http://dx.doi.org/10.1590/S1415-54192006000600007.
 

Submitted date:
02/25/2023

Accepted date:
03/20/2023

649c83eca953952357704633 rou Articles
Links & Downloads
1807-2577 (Electronic)
Rev. odontol. UNESP ©2024 All rights reserved.

Rev. odontol. UNESP

Share this page
Page Sections