Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.00522
Revista de Odontologia da UNESP
Original Article

Glass ionomer heated or not to identify bone defect created in rat calvaria

Ionômero de vidro aquecido ou não para identificação de defeitos ósseos criados em calvárias de ratos

Marcela Lucio CALDEIRA; Valéria Rodrigues FREITAS; José Ricardo SANTOS; Maria Júlia Giancursi ABONIZIO; Maria Fernanda do NASCIMENTO; Larissa Sgarbosa de Araújo MATUDA; Carolina dos Santos SANTINONI

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Abstract

Abstract: Introduction: Some experimental models have been used to evaluate the use of biomaterials in bone regeneration. Among them are the critical size defects (CSD) created in rat calvaria. An experimental model has been described in the literature, in which “L” markings are performed on the margins of the bone defects in order to assist in the precise identification of these defects during laboratory processing and analysis of the results. In the proposed model, the “L” markings are filled with amalgam.

Objective: The purpose of the present study was to evaluate the amalgam replacement of an experimental bony defect model in rat calvaria by heated or unheated glass ionomer.

Material and method: 24 rats were used. A 5 mm CSD was created at each animal calvaria. Two “L” shaped markings were made 2 mm from the margins of the bone defect, filled with amalgam (Group AM), heated glass ionomer cement (Group GIh) or not (Group GI). The animals were euthanized 15 days postoperatively. The areas of the surgical defect and the L-shaped marking were histomorphometrically analyzed and the data were analyzed statistically (p <0.05).

Result: There were no significant clinical, histological or methodological differences among the experimental groups.

Conclusion: It can be concluded that GI can replace AM in the proposed experimental model and GI heating did not promote additional benefits.

Keywords

Glass ionomer cements, dental amalgam, bone regeneration, rats

Resumo

Resumo: Introdução: Alguns modelos experimentais têm sido usados para avaliar o uso de biomateriais na regeneração óssea. Entre eles estão os defeitos de tamanho crítico (DTC) criados em calvárias de ratos. Um modelo experimental foi descrito na literatura onde marcações em L são realizadas nas margens do defeito ósseo para auxiliar na identificação precisa desses defeitos durante o processamento laboratorial e análise dos resultados. No modelo experimental proposto, as marcações em “L” são preenchidas com amálgama.

Objetivo: Avaliar a substituição do amálgama por ionômero de vidro aquecido ou não em um modelo experimental para identificação de defeito ósseo criado em calvária de ratos.

Material e método: Foram utilizados 24 ratos. Um DTC de 5 mm de diâmetro foi criado na calvária de cada animal. Duas marcações em “L” foram realizadas a 2 mm das margens do defeito ósseo, preenchidas com amálgama (Grupo AM), ionômero de vidro aquecido (Grupo CIVaq) ou não (Grupo CIV). Os animais foram eutanasiados aos 15 dias pós-operatórios. A área do defeito cirúrgico e das marcações em “L” foram histomorfometricamente avaliadas e os dados estatisticamente analisados (p<0,05).

Resultado: Não houve diferença estatisticamente significativa entre os grupos experimentais para as análises metodológicas, clínicas ou histomorfométrica realizadas.

Conclusão: Dentro dos limites deste estudo, pode-se concluir que CIV pode substituir o AM no modelo experimental proposto e o aquecimento do CIV não promoveu benefícios adicionais.
 

Palavras-chave

Cimentos de ionômeros de vidro, amálgama dentário, regeneração óssea, rats

References

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Submitted date:
02/10/2022

Accepted date:
04/26/2022

627e9271a9539560735cc085 rou Articles
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