Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.00520
Revista de Odontologia da UNESP
Original Article

Os efeitos da Punica granatum L. em diferentes concentrações sobre duas linhagens celulares: estudo in vitro

The effects of Punica granatum L. at different concentrations on two cell lines: in vitro study

Thaís de Oliveira ROCHA; Cristina WERKMAN; Hanna Flavia Santana dos SANTOS; Wagner de OLIVEIRA; Sigmar de Mello RODE

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Resumo

Resumo: Introdução: A Punica granatum L. (PG), utilizada como medicamento fitoterápico, apresenta propriedades terapêuticas, anti-inflamatórias e antioxidante. Embora diversos estudos já tenham sido realizados com este fitoterápico, seus possíveis efeitos citotóxicos nos tecidos humanos ainda não são claros.

Objetivo: Avaliar a citotoxicidade da PG por meio de cultura celular com duas linhagens: fibroblastos humanos de mucosa oral (FLM) e células de carcinoma epidermoide oral humano (KB).

Material e método: As células foram submetidas ao teste de viabilidade celular por 24 horas nas concentrações da PG 1%, 0,50%, 0,25%, 0,125%, 0,062% e 0,031%, e aos testes de citotoxicidade celular em 4 horas, 1, 3, 5 e 7 dias, em diferentes concentrações, realizados em triplicata. Foi utilizado um controle negativo (Triton 1%) e um controle positivo sem o extrato de PG. Os dados obtidos foram submetidos à ANOVA (p < 0,05).

Resultado: Foi possível observar que o extrato da PG possui efeitos inibitórios, apresentando-se maior nas células KB em relação às FLM. Os testes de citotoxicidade e viabilidade mostraram inibição e morte das células KB e FLM nas concentrações 1%, 0,50% e 0,25%.

Conclusão: O efeito inibitório da PG foi dose-dependentes, mostrando-se maior nas células KB em relação às FLM.

Palavras-chave

Punica granatum, fitoterapia, citotoxicidade, câncer

Abstract

Abstract: Introduction: Punica granatum L. (PG), used as a herbal medicine, has therapeutic, anti-inflammatory and antioxidant properties, although several studies have already been carried out with this herbal medicine, its possible cytotoxic effects on human tissues are still unclear.

Objective: To evaluate the cytotoxicity of PG through cell culture with two strains: human oral mucosa fibroblasts (LFM) and human oral squamous cell carcinoma (KB) cells.

Material and method: The cells were submitted to the cell viability test for 24 hours in the concentrations of PG 1%, 0.50%, 0.25%, 0.125%, 0.062% and 0.031% and the cell cytotoxicity tests in 4 hours, 1, 3, 5 and 7 days in different concentrations, performed in triplicate. A negative control (Triton 1%) and a positive control without the PG extract were used. The data obtained were submitted to ANOVA (p <0.05).

Result: it was possible to observe that the PG extract has inhibitory effects, being higher in KB cells in relation to LFM. The cytotoxicity and viability tests showed inhibition and death of KB and FLM cells at concentrations of 1%, 0.50% and 0.25%.

Conclusion: The inhibitory effect of PG was dose dependent, showing itself to be greater in KB cells compared to LMB.
 

Keywords

Punica granatum, phytotherapy, cytotoxicity, cancer

References

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Submitted date:
01/30/2020

Accepted date:
05/28/2020

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