Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.00520
Revista de Odontologia da UNESP
Original Article

Os efeitos da Punica granatum L. em diferentes concentrações sobre duas linhagens celulares: estudo in vitro

The effects of Punica granatum L. at different concentrations on two cell lines: in vitro study

Thaís de Oliveira ROCHA; Cristina WERKMAN; Hanna Flavia Santana dos SANTOS; Wagner de OLIVEIRA; Sigmar de Mello RODE

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Resumo

Resumo: Introdução: A Punica granatum L. (PG), utilizada como medicamento fitoterápico, apresenta propriedades terapêuticas, anti-inflamatórias e antioxidante. Embora diversos estudos já tenham sido realizados com este fitoterápico, seus possíveis efeitos citotóxicos nos tecidos humanos ainda não são claros.

Objetivo: Avaliar a citotoxicidade da PG por meio de cultura celular com duas linhagens: fibroblastos humanos de mucosa oral (FLM) e células de carcinoma epidermoide oral humano (KB).

Material e método: As células foram submetidas ao teste de viabilidade celular por 24 horas nas concentrações da PG 1%, 0,50%, 0,25%, 0,125%, 0,062% e 0,031%, e aos testes de citotoxicidade celular em 4 horas, 1, 3, 5 e 7 dias, em diferentes concentrações, realizados em triplicata. Foi utilizado um controle negativo (Triton 1%) e um controle positivo sem o extrato de PG. Os dados obtidos foram submetidos à ANOVA (p < 0,05).

Resultado: Foi possível observar que o extrato da PG possui efeitos inibitórios, apresentando-se maior nas células KB em relação às FLM. Os testes de citotoxicidade e viabilidade mostraram inibição e morte das células KB e FLM nas concentrações 1%, 0,50% e 0,25%.

Conclusão: O efeito inibitório da PG foi dose-dependentes, mostrando-se maior nas células KB em relação às FLM.

Palavras-chave

Punica granatum, fitoterapia, citotoxicidade, câncer

Abstract

Abstract: Introduction: Punica granatum L. (PG), used as a herbal medicine, has therapeutic, anti-inflammatory and antioxidant properties, although several studies have already been carried out with this herbal medicine, its possible cytotoxic effects on human tissues are still unclear.

Objective: To evaluate the cytotoxicity of PG through cell culture with two strains: human oral mucosa fibroblasts (LFM) and human oral squamous cell carcinoma (KB) cells.

Material and method: The cells were submitted to the cell viability test for 24 hours in the concentrations of PG 1%, 0.50%, 0.25%, 0.125%, 0.062% and 0.031% and the cell cytotoxicity tests in 4 hours, 1, 3, 5 and 7 days in different concentrations, performed in triplicate. A negative control (Triton 1%) and a positive control without the PG extract were used. The data obtained were submitted to ANOVA (p <0.05).

Result: it was possible to observe that the PG extract has inhibitory effects, being higher in KB cells in relation to LFM. The cytotoxicity and viability tests showed inhibition and death of KB and FLM cells at concentrations of 1%, 0.50% and 0.25%.

Conclusion: The inhibitory effect of PG was dose dependent, showing itself to be greater in KB cells compared to LMB.
 

Keywords

Punica granatum, phytotherapy, cytotoxicity, cancer

References

1 Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018 Nov;68(6):394-424. http://dx.doi.org/10.3322/caac.21492. PMid:30207593.

2 Ghose S, Radhakrishnan V, Bhattacharya S. Ethics of cancer care: beyond biology and medicine. Ecancermedicalscience. 2019 Mar;13:911. http://dx.doi.org/10.3332/ecancer.2019.911. PMid:31123494.

3 Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias BP Fo. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002 Oct;97(7):1027-31. http://dx.doi.org/10.1590/S0074-02762002000700017. PMid:12471432.

4 Ferreira SRG. Nutrição não sai de moda. Arq Bras Endocrinol Metabol. 2009 Jul;53(5):483-4. http://dx.doi.org/10.1590/S0004-27302009000500001. PMid:19768239.

5 Nascimento BJ Jr, Tínel LO, Silva ES, Rodrigues LA, Freitas TON, Nunes XP, et al. Avaliação do conhecimento e percepção dos profissionais da estratégia de saúde da família sobre o uso de plantas medicinais e fitoterapia em Petrolina-PE, Brasil. Rev Bras Plantas Med. 2016 Mar;18(1):57-66. http://dx.doi.org/10.1590/1983-084X/15_031.

6 Sharma P, McClees SF, Afaq F. Pomegranate for prevention and treatment of cancer: an update. Molecules. 2017 Jan;22(1):177. http://dx.doi.org/10.3390/molecules22010177. PMid:28125044.

7 Shaygannia E, Bahmani M, Zamanzad B, Rafieian-Kopaei M. A review study on Punica granatum L. J Evid Based Complementary Altern Med. 2016 Jul;21(3):221-7. http://dx.doi.org/10.1177/2156587215598039. PMid:26232244.

8 Turrini E, Ferruzzi L, Fimognari C. Potential effects of pomegranate polyphenols in cancer prevention and therapy. Oxid Med Cell Longev. 2015;2015:938475. http://dx.doi.org/10.1155/2015/938475. PMid:26180600.

9 Mandal A, Bishayee A. Mechanism of breast cancer preventive action of pomegranate: disruption of estrogen receptor and Wnt/β-catenin signaling pathways. Molecules. 2015 Dec;20(12):22315-28. http://dx.doi.org/10.3390/molecules201219853. PMid:26703530.

10 Khwairakpam AD, Bordoloi D, Thakur KK, Monisha J, Arfuso F, Sethi G, et al. Possible use of Punica granatum (Pomegranate) in cancer therapy. Pharmacol Res. 2018 Jul;133:53-64. http://dx.doi.org/10.1016/j.phrs.2018.04.021. PMid:29729421.

11 Joshi C, Patel P, Kothari V. Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens. F1000 Res. 2019 Apr;8:70. http://dx.doi.org/10.12688/f1000research.17430.2. PMid:30828441.

12 Semwal DK, Semwal RB, Combrinck S, Viljoen A. Myricetin: a dietary molecule with diverse biological activities. Nutrients. 2016 Feb;8(2):90. http://dx.doi.org/10.3390/nu8020090. PMid:26891321.

13 Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, et al. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem. 2005 Jun;16(6):360-7. http://dx.doi.org/10.1016/j.jnutbio.2005.01.006. PMid:15936648.

14 Syed DN, Chamcheu JC, Adhami VM, Mukhtar H. Pomegranate extracts and cancer prevention: molecular and cellular activities. Anticancer Agents Med Chem. 2013 Oct;13(8):1149-61. http://dx.doi.org/10.2174/1871520611313080003. PMid:23094914.

15 Li Y, Yang F, Zheng W, Hu M, Wang J, Ma S, et al. Punica granatum (pomegranate) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in non-small cell lung cancer in vitro. Biomed Pharmacother. 2016 May;80:227-35. http://dx.doi.org/10.1016/j.biopha.2016.03.023. PMid:27133061.

16 Saeed M, Naveed M, BiBi J, Kamboh AA, Arain MA, Shah QA, et al. The Promising pharmacological effects and therapeutic/medicinal applications of Punica granatum L. (Pomegranate) as a functional food in humans and animals. Recent Pat Inflamm Allergy Drug Discov. 2018;12(1):24-38. http://dx.doi.org/10.2174/1872213X12666180221154713. PMid:29473532.

17 Shirode AB, Bharali DJ, Nallanthighal S, Coon JK, Mousa SA, Reliene R. Nanoencapsulation of pomegranate bioactive compounds for breast cancer chemoprevention. Int J Nanomedicine. 2015 Jan;10:475-84. http://dx.doi.org/10.2147/IJN.S65145. PMid:25624761.

18 Badawi NM, Teaima MH, El-Say KM, Attia DA, El-Nabarawi MA, Elmazar MM. Pomegranate extract-loaded solid lipid nanoparticles: design, optimization, and in vitro cytotoxicity study. Int J Nanomedicine. 2018 Mar;13:1313-26. http://dx.doi.org/10.2147/IJN.S154033. PMid:29563789.
 


Submitted date:
01/30/2020

Accepted date:
05/28/2020

5f16e77b0e882518739517e2 rou Articles
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