Revista de Odontologia da UNESP
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Revista de Odontologia da UNESP
Original Article

Interaction between Escherichia coli and Candida albicans in biofilms formed in vitro: analysis of cell viability by the colorimetric assay

Interação entre Escherichia coli e Candida albicans em biofilmes formados in vitro: análise da viabilidade celular por método colorimétrico

Piva, Elisabete; Barbosa, Junia de Oliveira; Rossoni, Rodnei Dennis; Vilela, Simone Furgeri Godinho; Jorge, Antonio Olavo Cardoso; Junqueira, Juliana Campos

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Abstract

The interactions between fungi and bacteria are present in nature and has medical and environmental importance. The aim of this study was to evaluate the effects of interaction between ATCC strains Candida albicans and Escherichia coli through the study of biofilms in vitro. For this study were developed biofilms formed by the association between C.albicans and E.coli and monotypic biofilms of C. albicans (control group). To evaluate the effects of E.coli in biofilms of C.albicans, we tested different cell densities of E.coli suspension (107, 106 and 105 cells/mL). After 90 minutes and 24 hours of biofilm formation, cell viability of C. albicans was quantified using the XTT (2 methoxy 4 nitro 5 sulfophenyl 5 phenylamino carbonyl 2H  tetrazolium hydroxide).) colorimetric assay. Data were submitted to ANOVA and Tukey's test, with 5% of significance. After 90 min of Candidal biofilm formation, we observed that cell viability of C. albicans heterotypic biofilms was similar to monotypic biofilm (control group). For the analysis of 24 hours, it was found that cell viability of C.albicans was higher in the monotypic biofilm if compared to heterotypic biofilm, however this difference was not statistically significant. In addition, there were no statistical differences between E. coli different cell densities tested in heterotypic biofilms. According to the methods used, we can conclude that E. coli did not inhibit the biofilm formation by C. albicans.

Keywords

Candida albicans, Escherichia coli, biofilm

Resumo

As interações entre fungos e bactérias estão presentes na natureza e têm grande relevância médica e ambiental. O objetivo deste estudo foi avaliar os efeitos de Escherichia coli sobre os biofilmes de Candida albicans formados in vitro. Foram desenvolvidos biofilmes heterotípicos com a associação de C. albicans e E. coli e biofilmes monotípicos de C. albicans (grupo controle). Foi utilizada uma suspensão padronizada de C. albicans contendo 107 células/mL para formação dos biofilmes. Para analisar os efeitos de E. coli no biofilme de C. albicans, foram testadas diferentes densidades celulares da suspensão de E. coli (107, 106 e 105 células/mL). Após 90 minutos e 24 horas da formação do biofilme, a viabilidade celular de C. albicans foi quantificada utilizando-se o ensaio colorimétrico XTT (2 metoxi 4 nitro 5 sulfofenil 5 fenilalanina carbonil 2H tetrazolium hidróxido). Os dados foram submetidos à Análise de Variância ANOVA e ao teste de Tukey, com significância de 5% (p < 0,05). Após 90 minutos da formação do biofilme, observou-se que a viabilidade celular de C. albicans dos biofilmes heterotípicos foi semelhante ao monotípico. A análise de 24 horas demonstrou que a viabilidade celular de C. albicans foi maior no biofilme monotípico em relação aos biofilmes heterotípicos; entretanto, essa diferença não foi estatisticamente significante. Além disso, não foram observadas diferenças estatísticas entre as densidades celulares de E. coli testadas nos biofilmes heterotípicos. Conclui-se que, dentro dos parâmetros utilizados, E. coli não inibiu a formação de biofilme por C. albicans.

Palavras-chave

Candida albicans, Escherichia coli, biofilme.

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