Revista de Odontologia da UNESP
Revista de Odontologia da UNESP
Original Article

Antifungal activity of the essential oils from Eucalyptus globulus L. on Candida spp.

Atividade antifúngica in vitro do óleo essencial de Eucalyptus globulus L. sobre Candida spp.

Castro, Ricardo Dias de; Lima, Edeltrudes de Oliveira

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Considering the need to obtain new therapeutic agents aimed at acting against oral candidiasis, the objective of this study was to investigate the in vitro antifungal activity of the essential oil from Eucalyptus globulus L. (Eucalyptus) on Candida species involved in oral infections. Therefore, microbiological assays with the purpose of determining the Minimum Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC) were performed, respectively, by using the technique of microdilution and the technique of colonies forming units growth on solid medium. For the essential oil from E. globulus L. were observed MIC of 312.5 µg.mL–1 for 76.2% of the Candida strains and MFC of 625 µg.mL–1 for 81% of the strains under study. All strains were sensitive to the synthetic antifungal agents, miconazole and nystatin, used as controls. From the conditions studied and from the results obtained, it’s concluded that the essential oil from E. globulus L. showed antifungal activity on the Candida species.


Eucalyptus globulus L., oral candidiasis, Candida spp., stomatitis on prosthesis, product with antimicrobial action


Considerando a necessidade de obtenção de novos agentes terapêuticos voltados para o combate da candidose bucal, o objetivo deste estudo foi investigar a atividade antifúngica in vitro do óleo essencial do Eucalyptus globulus L. (eucalipto) frente a espécies de Candida envolvidas com infecções bucais. Para tanto, ensaios microbiológicos com finalidade de determinar a Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) foram realizados, respectivamente, utilizando as técnicas da microdiluição e do crescimento de unidades formadores de colônias em meio sólido. Para o óleo essencial de E. globulus L. foram observadas CIM de 312,5 µg.mL–1 para 76,2% das cepas de Candida e CFM de 625 µg.mL–1 para 81% das cepas ensaiadas. Todas as cepas foram sensíveis aos antifúngicos sintéticos, miconazol e nistatina, utilizados como controles. Diante das condições estudadas e dos resultados obtidos, foi possível concluir que o óleo essencial de E. globulus L. apresentou atividade antifúngica sobre as espécies de Candida.


Eucalyptus globulus L., candidíase bucal, Candida spp., estomatite sobre prótese, produto com ação antimicrobiana


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