Antifungal activity of the essential oils from Eucalyptus globulus L. on Candida spp.
Atividade antifúngica in vitro do óleo essencial de Eucalyptus globulus L. sobre Candida spp.
Castro, Ricardo Dias de; Lima, Edeltrudes de Oliveira
Rev. odontol. UNESP, vol.39, n3, p.179-184, 2010
Abstract
Considering the need to obtain new therapeutic agents aimed at acting against oral candidiasis, the objective of this study was to investigate the in vitro antifungal activity of the essential oil from Eucalyptus globulus L. (Eucalyptus) on Candida species involved in oral infections. Therefore, microbiological assays with the purpose of determining the Minimum Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC) were performed, respectively, by using the technique of microdilution and the technique of colonies forming units growth on solid medium. For the essential oil from E. globulus L. were observed MIC of 312.5 µg.mL–1 for 76.2% of the Candida strains and MFC of 625 µg.mL–1 for 81% of the strains under study. All strains were sensitive to the synthetic antifungal agents, miconazole and nystatin, used as controls. From the conditions studied and from the results obtained, it’s concluded that the essential oil from E. globulus L. showed antifungal activity on the Candida species.
Keywords
Eucalyptus globulus L., oral candidiasis, Candida spp., stomatitis on prosthesis, product with antimicrobial action
Resumo
Considerando a necessidade de obtenção de novos agentes terapêuticos voltados para o combate da candidose bucal, o objetivo deste estudo foi investigar a atividade antifúngica in vitro do óleo essencial do Eucalyptus globulus L. (eucalipto) frente a espécies de Candida envolvidas com infecções bucais. Para tanto, ensaios microbiológicos com finalidade de determinar a Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) foram realizados, respectivamente, utilizando as técnicas da microdiluição e do crescimento de unidades formadores de colônias em meio sólido. Para o óleo essencial de E. globulus L. foram observadas CIM de 312,5 µg.mL–1 para 76,2% das cepas de Candida e CFM de 625 µg.mL–1 para 81% das cepas ensaiadas. Todas as cepas foram sensíveis aos antifúngicos sintéticos, miconazol e nistatina, utilizados como controles. Diante das condições estudadas e dos resultados obtidos, foi possível concluir que o óleo essencial de E. globulus L. apresentou atividade antifúngica sobre as espécies de Candida.
Palavras-chave
Eucalyptus globulus L., candidíase bucal, Candida spp., estomatite sobre prótese, produto com ação antimicrobiana
References
1. Thein ZM, Samaranayake YH, Samarayake LP. Effect of oral bacteria on growth and survival of Candida albicans biofilms. Arch Oral Biol. 2006; 51: 672-80.
2. Henriques M, Azeredo J, Oliveira R. Adhesion of Candida albicans and Candida dubliniensis to acrylic and hydroxyapatite. Biointerfaces. 2004; 33: 235-41.
3. Ramage G, Saville SP, Thomas DP, Lopez-Ribot JL. Candida biofilms: an update. Eukaryot Cell. 2005; 4: 633-8.
4. Monge RA, Román E, Nombela C, Pla J. The MAP Kinase signal transduction network in Candida albicans. Microbiol. 2006; 152: 905-12.
5. Müller V, Viemann D, Schmidt M, Endres N, Ludwing S, Leverkus M, et al. Candida albicans triggers activation of distinct signaling pathways to establish a proinflammatory gene expression program in primary human endothelial cells. J Immunol. 2007; 179: 8435-45.
6. Orozco A, Zhou X, Filler SG. Mechanisms of the proinflammatory response of endothelial cells to Candida albicans infection. Infect Immun. 2000; 68: 1134-41.
7. Wingeter MA, Guilhermetti E, Shinobu CS, Takaki I, Svdzinski TIE. Identificação microbiológica e sensibilidade in vitro de Candida isoladas da cavidade oral. Rev Soc Bras Med Trop. 2007; 40: 272-6.
8. Marichal P, Koymans L, Willemsens S, Bellens D, Verhasselt P, Luyten W, et al. Contribution of mutations in the cytochrome P450 14α-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans. Microbiol. 1999; 145: 2701-13.
9. Khan R, Islam B, Akram M, Shakil S, Ahmad A, Manazir AS, et al. Antimicrobial activity of five herbal extracts against multi drug resistant (MRD) strains of bacteria and fungus of clinical origin. Molecules. 2008; 13: 1-12.
10. Agarwal V, Lal EP, Pruthi EV. Prevention of Candida albicans biofilm by plant oils. Mycopathologia. 2008; 165: 13-9.
11. Alzamora L, Morales L, Armas L, Fernandez G. Medicina tradicional en el Perú: actividade antimicrobiana in vitro de los aceites esenciales extraídos de algunas plantas aromáticas. An Fac Med (Peru). 2001; 62: 156-61.
12. Ghedira K, Goetz P, Jeune RL. Eucalyptus globulus Labill. Phytothér. 2008; 6: 197-200.
13. Sartorelli P, Marquioreto AD, Amaral-Baroli A, Lima MEL, Moreno PRH. Chemical composition and antimicrobial activity of the essential oils from two specieis of Eucalyptus. Phytother Res. 2007; 21: 199-299.
14. Silva J, Abebe W, Sousa SM, Duarte VG, Machado MIL, Matos JA. Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J Ethnopharmacol. 2003; 89: 277-83.
15. Ogunwande IA, Olowore NO, Ekundayo O, Walker TM, Schmid JM, Setzer WN, et al. Studies on the essential oils composition, antibacterial and cytotoxicity of Eugenia uniflora L. Int J Aromather. 2005; 15: 147-52.
16. Carson CF, Cookson BD, Farrelly HD, Riley TV. Susceptibility of methicillin-resistant Staphylococcus aureus to the essential oil of Melaleuca alternifolia. J Antimicrob Chemother. 1995; 35: 421-4.
17. Nikawa H, Yamasiro H, Egusa H, Furukawa M, Setijato D, Hamada T. In vitro cariogenic potential of Candida albicans. Mycoses. 2003; 46: 471-8.
18. Baumgartner J, Watts C, Xia T. Occurrence of Candida albicans in infection of endodontic origin. J Endod. 2000; 26: 695-8.
19. Hannula J, Dogan B, Slots J, Okte E, Asikainen S. Subgengival strains of Candida albicans in relation to geographical origin and occunrence of periodontal pathogenic bacteria. Oral Microbiol Immunol. 2001; 16: 113-8.
20. Martins CAP, Santos SSF, Loberto J, Hoga-Ito CY, Jorge AOC. Presença de Candida spp. em pacientes com periodontite crônica. Cienc Odontol Bras. 2002; 5: 75-83.
21. Devienne KF, Raddi MSG Screening for antimicrobial activity of natural products using a microplate photometer. Braz J Microbiol. 2002; 33: 166-8.
22. Silver L, Bostian K. Screening of natural products for antimicrobial agents. Eur J Clin Microbiol Infect Dis. 1990; 9: 455-61.
23. Dutta BK, Karmakar S, Naglot A, Aicha JC, Begam M. Anticandidial activity if some essential oils of a mega biodiversity hotspot in India. Mycoses. 2007; 50: 121-4.
24. Brantner AH, Asres K, Chakraborty A, Tokuda H, Mou XY, Mukainaka T, et al. Grown gall – a plant tumour with biological activities. Phytother Res. 2003; 12: 299-436.
25. Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med. 2006; 6(39): 1-8.
26. Allegrini J, Bouchberg MS, Mailols H. Émulsiond d’huilles essentielles fabricaton et applications en microbiologie. Soc Pharm Montpellier. 1973; 33: 73-86.
27. National Committee for Clinical Laboratory Standard. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, Approved Standard. 6th ed. v. 23-M7-A6, 2003.
28. Deswal DP, Chand U. Standardization of the tetrazolium test for viability estimation in rice bean (Vigna umbellate T.) seeds. Seed Science and Technology. 1997; 25: 409-417.
29. Gabre DF. Manual do teste de tetrazólio. Brasília: AGIPLAN; 1976.
30. Denning DW, Hanson LH, Perlaman AM, Stevens DA. In vitro susceptibility and synergy studies of Aspergillus species to conventional and new agents. Diagn Microbiol Infec Dis. 1992; 15: 21-34.
31. Rasooli I, Abyaneh MR. Inhibitory effects of thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Control. 2004; 15: 479-83.
32. Lemos JA, Costa CR, Araújo CR, Souza LKH, Silva MRR. Susceptibilty testing of Candida albicans isolated from oropharyngeal mucosa of HIV+ patients to fluconazole, amphotericin B and caspofungin, killing kinetics of caspofungin and amphotericin against fluconazole resistant and susceptible isolates. Braz J Microbiol. 2009; 40: 163-9.
33. Marsh P, Martin MV. Microbiologia oral. São Paulo: Santos; 2005.
34. Nascimento PFF, Rodrigues ARA, Santos PO, Barbosa-Júnior A, Trindade RC. Atividade antimicrobiana dos óleos essenciais: uma abordagem multifatorial dos métodos. Rev Bras Farmacogn. 2007; 17: 108-13.
35. Lima IO, Oliveira RAG, Lima EO, Farias NMP, Souza EL. Atividade antifúngica de óleos essenciais sobre espécies de Candida. Rev Bras Farmacogn. 2006; 16: 197-201.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email