Revista de Odontologia da UNESP
Revista de Odontologia da UNESP
Original Article

Development of caries-like lesions in human and bovine dentin compared to natural caries

Desenvolvimento de lesões artificiais de cárie em dentina humana e bovina comparada a lesões de cárie natural

Rehder-Neto, Francisco Carlos; Menezes, Márcio de; Chimello, Daniela Thomazatti; Serra, Mônica Campos

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Due to the complexity, ethical aspects and high costs involving clinical experiments on dental caries, in vitro studies have been considered as an alternative option to clinical researches. The purpose of this study was to compare artificial caries-like lesions in human and bovine dentin to natural caries in human dentin (CT). Fifteen specimens of human dentin with natural caries, 45 specimens of human dentin (H), and 45 specimens of bovine dentin (B) were flattened and polished. The specimens from groups H and B were submitted to three different protocols of pH cycles: 8 (H8 and B8), 12 (H12 and B12), and 16 cycles (H16 and B16). Each cycle consisted of immersion of the specimens for 6 hours in a demineralising solution and for 18 hours in a remineralising solution. After the cariogenic challenge, the Knoop microhardness (KHN) was determined at 30, 60, 90, 120 and 150 μm from the lesion surface. The Analysis of Variance showed a significant effect of the interaction cycle-depth. The Student’s t-test was employed to compare the mean values of KHN from the substrate with natural caries to the values of KHN of each cycle of the experimental groups (H or B), at each depth. The pH-cycling models closest to natural caries were group B8, for bovine dentin and group H16, for human dentin. Within the conditions of the present study, it can be concluded that the use of both human and bovine substrates can be considered a viable alternative to the development of artificial caries lesions.


Microhardness, human dentin, bovine dentin, dental caries


Dada a complexidade, aspectos éticos e custos envolvidos na condução de experimentos clínicos relacionados a cárie dental, estudos in vitro têm sido uma alternativa às pesquisas clínicas. O objetivo deste estudo foi avaliar o desenvolvimento de lesões artificiais de cárie em dentina humana e bovina, comparado à lesões de cárie natural em dentina humana (CT). Quinze fragmentos de dentina humana naturalmente cariada, 45 fragmentos de dentina humana (H) e 45 fragmentos de dentina bovina (B) foram planificados e polidos. Os espécimes dos grupos H e B foram então submetidos a três protocolos de ciclos de pH: 8 (H8 e B8), 12 (H12 e B12) e 16 ciclos (H16 e B16). Após o desafio cariogênico, valores de microdureza Knoop (KHN) foram obtidos a 30, 60, 90, 120 e 150 µm da superfície das lesões. A Análise de Variância mostrou efeito significativo da interação ciclo-profundidade. O teste t-Student foi empregado para comparar as médias de KHN do substrato naturalmente cariado (CT) em relação a cada desafio cariogênico desenvolvido nos grupos H e B, em cada profundidade. Os modelos de ciclos de pH que mais se aproximaram em termos de microdureza das lesões de cárie natural foram o grupo B8, para dentina bovina e o grupo H16, para dentina humana. Dentro das condições do presente estudo, pode-se concluir que a utilização tanto de substrato humano quanto bovino pode ser considerada uma alternativa viável para o desenvolvimento de lesões de cárie artificial.


Microdureza, dentina humana, dentina bovina, cárie dental


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