Revista de Odontologia da UNESP
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Revista de Odontologia da UNESP
Original Article

Comparison between liquid based cytology and conventional exfoliative cytology in the diagnosis of oral candidoses

Comparação entre a citologia em base líquida e a citologia esfoliativa convencional no diagnóstico da candidose bucal

Sandrin, Rodrigo; Campagnoli, Eduardo Bauml; França, Beatriz Helena Sottile; Lima, Antonio Adilson Soares de

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Abstract

The aim of this study was to compare the efficiency of the liquid based cytology (LBC) and conventional exfoliative cytology (CEC) in the diagnosis of oral candidosis. Material and method: The sample was divided in two groups: a) experimental group – composed by 27 individuals with oral candidosis (submitted to LBC and CEC) and b) control group – composed by 30 individuals with no oral lesion (submitted to LBC). Besides, the quality of the cytological smears; the association between hyphae and/or pseudohyphae with the type of epithelial cell; and the efficacy of the two methods after the treatment with antifungal medication were evaluated. The smears were processed in the laboratory, analyzed by light microscopy and submitted to statistical analysis using Chi‑square test (P < 0.05). Result: There were more smears exhibiting hyphaes and/or pseudohyphaes in LBC than CEC. Besides, the quantity and the more uniform dispersion of the epithelial cells on the slide were more present. Thus, this method showed the best quality of the cytological smears. When the association between hyphae and/or pseudohyphae with the type of epithelial cell was analyzed, both LBC (experimental and control groups) and CEC demonstrated that these structures were more associated with nucleated superficial and intermediary epithelial cells. Conclusion: Even so LBC has been technically superior than CEC to the preparing of best quality slides and favoring to the detection of hyphae and/or pseudohyphae. Nevertheless, it did not show satisfactory results as a safe method to the diagnosis of oral candidosis.

Keywords

Cytology, candidiasis, oral diagnosis, mouth mucosa, oral health.

Resumo

Objetivo: O objetivo deste estudo foi comparar a eficácia da citologia esfoliativa em base-líquida (CBL) e da citologia esfoliativa convencional (CEC) no diagnóstico da candidose bucal. Material e método: a amostra foi dividida em dois grupos: a) grupo experimental – formado por 27 indivíduos com candidose bucal (submetidos a CBL e CEC) e b) grupo controle – composto por 30 indivíduos sem nenhuma lesão bucal (submetidos a CBL). Além de avaliar a eficácia dos métodos no diagnóstico de candidose bucal, procurou-se comparar a qualidade das imagens microscópicas, a associação da presença de hifas e/ou pseudohifas com o tipo de célula epitelial e a eficácia de aplicação dos dois métodos após o tratamento com antifúngico. Os esfregaços foram processados em laboratório, analisados por microscopia de luz e submetidos à análise estatística por meio do teste do Qui-quadrado (p < 0,05). Resultado: Os resultados mostraram que a CBL foi mais eficaz do que a CEC devido ao maior número de lâminas contendo hifas e/ou pseudohifas, bem como pela quantidade e dispersão mais uniformes das células epiteliais, o que caracterizou a qualidade superior das imagens neste método. Ao se avaliar a associação de hifas e/ou pseudohifas com o tipo de célula epitelial mais encontrado, observou-se que tanto na CBL quanto na CEC as referidas estruturas estavam mais associadas com células das camadas superficial nucleada e intermediária. Conclusão: embora a
citologia em base líquida tenha sido tecnicamente superior à citologia esfoliativa convencional na confecção de lâminas com melhor qualidade e favorecendo a detecção de hifas e pseudohifas, a CBL não demonstrou resultados satisfatórios como um método seguro para o diagnóstico da candidose oral.

Palavras-chave

Citologia, candidíase, diagnóstico bucal, mucosa bucal, saúde bucal.

References



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