Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/588018497f8c9d0a098b4b5d
Revista de Odontologia da UNESP
Original Article

Concentração de flúor no esmalte adjacente a diferentes materiais restauradores diretos após desafio cariogênico

Fluoride concentration on enamel adjacent to different direct restorative materials after in vitro cariogenic challenge with Streptococcus mutans

Paradella, T.C.; Koga Ito, C.Y.; Jorge, A.O.C.

Downloads: 1
Views: 929

Resumo

O objetivo deste estudo foi avaliar a concentração de flúor no esmalte dentário humano adjacente a diferentes materiais restauradores após desafio cariogênico in vitro com Streptococcus mutans. Para isso, 72 terceiros molares humanos, recém-extraídos receberam preparos classe V padronizados e foram restaurados conforme os grupos: RC-Z: resina composta convencional Z250; RC-F: resina composta modificada por poliácidos Freedom; CIV-V: ionômero de vidro modificado por resina Vitremer: CIV-F: cimento de ionômero de vidro convencional Fuji IX. Foram cortados espécimes de 4 x 4 x 2 mm e subdivididos em Grupo controle (n = 6 mantidos em umidade relativa 100% a 37 °C durante todo o experimento) e Grupo experimental (n = 12). No Grupo experimental, os espécimes foram submetidos ao desafio cariogênico com cultura de S. mutans ATCC 35688 em caldo contendo sacarose a 5%, sendo este caldo substituído a cada 48 horas. Após 14 dias, os espécimes foram imersos em solução remineralizante por 14 dias, sendo então desidratados, metalizados e submetidos à espectroscopia por dispersão de raio X (EDS). Os resultados foram submetidos à análise estatística Kruskall-Wallis e Student-Newman-Keuls (α = 5%) e demonstraram que somente RC-Z apresentou diferença entre o Grupo controle e experimental (p = 0,0037). No Grupo experimental, houve diferença entre RC-Z e RC-F (p = 0,0450), RC-Z e CIV-V (p = 0,0107); RC-Z e CIV-F (p < 0,0001), demonstrando que a concentração de flúor no esmalte adjacente a CIV-V e CIV-F foi superior a dos materiais resinosos, que não apresentaram alta concentração de flúor no esmalte após desafio cariogênico, em comparação aos outros materiais.

Palavras-chave

Streptococcus mutans, cárie, esmalte, flúor

Abstract

The purpose of this study was to evaluate the concentration of fluoride on human tooth enamel adjacent to different direct restorative materials after in vitro cariogenic challenge with Streptococcus mutans. For that, 72 freshly-extracted human third molars received standard class V preps and were restored according to the groups: RC-Z: Z250 conventional composite resin; RC-F: Freedom polyacid-modified composite resin; CIV-V: Vitremer resin-modified glass-ionomer: CIV-F: Fuji IX conventional glass-ionomer cement. Specimens of 4 x 4 x 2 mm were cut and subdivided into control Group (n = 6 kept in 100% relative humidity at 37 °C during all the experiment) and experimental Group (n = 12). In the experimental Group, specimens were submitted to cariogenic challenge with S. mutans ATCC 35688 in 5% sucrose broth, being this broth replaced every 48 hours. After 14 days, specimens were immersed in remineralizing solution for 14 days, being then dehydrated, gold-sputtered and submitted to X-ray diffraction spectroscopy. Results were statistically analyzed by Kruskall-Wallis and Student-Newman-Keuls (α = 5%) tests and showed that only Group RC-Z presented difference between control and experimental Group (p = 0.0037). In the experimental Group, there were differences between RC-Z and RC-F (p = 0.0450), RC-Z and CIV-V (p = 0.0107); RC-Z and CIV-F (p < 0.0001), showing that the fluoride concentration on enamel adjacent to CIV-V and CIV-F was higher than the resinous materials, which did not present high fluoride concentration on enamel after cariogenic challenge, in comparison to the other materials

Keywords

Streptococcus mutans, caries, enamel, fluoride

References



1. Nogueira EC, Serra MC, Morais PMR, Marchi GM. Avaliação in vitro da ação cariostática de híbridos de ionômero de vidro/resina composta. Rev Paul Odontol. 2001;23(3): 30-3.

2. Terada RSS, Navarro MFL, Carvalho RM, Taga E, Fernandes RBDH. Avaliação in vitro da liberação de flúor de cimentos de ionômero de vidro e outros materiais que contêm flúor. Rev Odontol Univ São Paulo. 1998;12:81-9.

3. Pereira IVA, Ribeiro PEBC, Paravini A, Tárzia O. Liberação de flúor por dois cimentos de ionômero de vidro com relação às proteções por presa – estudo in vitro. Rev Fac Odontol Bauru. 1999;7(3/4):21-6.

4. Ten Cate JM, Mundoff-Shrestha AS. Working group report 1: laboratory models for caries (in vitro and animal models). Adv Dent Res. 1995;9:323-4.

5. Francci C, Deaton TG, Arnold RR, Swift EJ Jr, Perdigão J, Bawden JW. Fluoride release from restorative materials and its effects on dentin demineralization. J Dent Res. 1999;78:1647-54.

6. Lennon AM, Wiegand A, Buchalla W, Attin T. Approximal caries development in surfaces in contact in fluoride-releasing and non-fluoride releasing restorative materials: an in situ study. Eur J Oral Sci. 2007;115:497-501.

7. Serra MC, Rodrigues Júnior AL. Potencial cariostático de materiais restauradores contendo flúor. Rev Assoc Paul Cir Dent. 1998;52:359-63.

8. Arnold WH, Sonkol T, Zoellner A, Gaengler P. Comparative study of in vitro caries-like lesions and natural caries lesions at crown margins. J Prosthodont. 2007;16(6):1-7.

9. Asmussen E, Peutzfeldt A. Direction of shrinkage of light-curing resin composites. Acta Odontol Scand. 1999;57:310-5.

10. Liporoni P, Paulilo LAMS, Cury JA, Santos Dias CT, Paradella TC. Surface finishing of resin-modified glass ionomer. Gen Dent. 2003;51:541-3.

11. Rodrigues LK, Cury JA, Santos MN. The effect of gamma radiation on enamel hardness and its resistance to demineralization in vitro. J Oral Sci. 2004;46:215-20.

12. Gibbons RJ, Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968;13:1249-62.

13. Carreto CFP, Navas EAFA, Paradella TC, Oliveira LD, Junqueira JC, Jorge AOC. Efeitos do chá de tomilho sobre a aderência in vitro de Streptococcus mutans ao esmalte dentário e Candida albicans à resina acrílica. Rev Odontol UNESP. 2007;36:281-6.

14. Itota T, Nakabo S, Narukami T, Tashiro Y, Torii Y, McCabe JF, et al. Effect of two-step adhesive systems on inhibition of secondary caries around fluoride-releasing resin composite restoration in root dentin. J Dent. 2005;33:147-54.

15. Seeman R, Bizhang M, Klück I, Loth J, Roulet JF. A novel in vitro microbial-based model for studying caries formation – development and initial testing. Caries Res. 2005;39:185-90.

16. Samarawickrama DYD. Saliva substitutes: how effective and safe are they? Oral Dis. 2002;8:177-9.

17. Eick S, Glockmann E, Brandl B, Pfister W. Adherence of Streptococcus mutans to various restorative materials in a continuous flow system. J Oral Rehabil. 2004;31:278-85.

18. Paradella TC, Fava M. Bond strength of adhesive systems to human tooth enamel. Braz Oral Res. 2007;21(1):4-9.

19. Pelino JEP, Mello JB, Eduardo CP, Jorge AOC. In vitro study of the Nd:YAG laser effect on human dental enamel: optical and scanning electron microscope analysis. J Clin Laser Med Surg. 1999;17:171-7.

20. Okazaki M. Chrystallographic morphology of heteregenous fluoridated carbonate apatites. J Dent Res. 1993;72:1285-90.

21. Massara MLA, Alves JB, Brandão PRG. Atraumatic restorative treatment: clinical, ultrastructural and chemical analysis. Caries Res. 2002;36:430-6.

22. Asaka Y, Miyazaki M, Aboshi H, Yoshida T, Takamizawa T, Kurokawa H, et al. EDX fluorescence analysis and SEM observation of resin composites. J Oral Sci. 2004;46:143-8.

23. Fu B, Yuan J, Qian W, Shen Q, Sun X, Hannig M. Evidence of chemisorption of maleic acid to enamel and hydroxyapatite. Eur J Oral Sci. 2004;112:362-7.

24. Statham PJ. A check total for validating standardless and normalized EDX analysis at low kv. Microchim Acta. 2004;145:229-35.

25. Tantbirojn D, Douglas WH, Ko CC, McSwiggen PL. Spatial chemical analysis of dental stain using wavelength dispersive spectrometry. Eur J Oral Sci. 1998;106:971-6.

26. Herrera M, Castillo A, Baça P, Carrion P. Antibacterial activity of glass-ionomer restorative cements exposed to cavity-producing microorganisms. Oper Dent. 1999;24:286-91.
588018497f8c9d0a098b4b5d rou Articles
Links & Downloads

Rev. odontol. UNESP

Share this page
Page Sections