Atividade antimicrobiana de um novo biomaterial utilizado na remoção químico-mecânica da cárie
Antimicrobial activity of a new biomaterial used for chemomechanical caries removal
Bortoletto, C.C.; Ferrari, J.C.L.; Motisuki, C.; Spolidorio, D.M.P.; Santos-Pinto, L.A.M.
Rev. odontol. UNESP, vol.34, n4, p.199-201, 2005
Resumo
O objetivo deste trabalho foi avaliar, in vitro, a capacidade antimicrobiana do Papacárie®, para Streptococcus mutans e Lactobacillus acidophilus, por meio da técnica de difusão em agar. Trezentos microlitros de Streptococcus mutans foram inseridos em 15 mL de meio de cultura BHI-ágar e dispensados em seis placas de Petri. O mesmo procedimento foi realizado para Lactobacillus acidophilus. Após endurecimento do meio, foram confeccionados seis poços por placa, sendo o material experimental inserido alternadamente com gel de gluconato de clorexidina a 1% (controle positivo). As placas foram mantidas à temperatura ambiente por duas horas para pré-difusão do material e posteriormente incubadas a 37°C por 24 horas em microaerofilia. Após esse período, o halo de inibição formado ao redor do poço foi medido com o auxílio de paquímetro digital. A análise dos dados obtidos revelou que o Papacárie® não apresentou propriedades antimicrobianas para Streptococcus mutans e Lactobacillus acidophilus.
Palavras-chave
Streptococcus mutans, Lactobacillus acidophilus, papaína.
Abstract
The aim of this in vitro study was to evaluate the antimicrobial potential of Papacárie®, for Streptococcus mutans and Lactobacillus acidophilus, with the agar diffusion test. Three hundred microliters of Streptococcus mutans were introduced in 15 mL of BHI-ágar culture media and transferred to six Petri plates. The same procedure was done for Lactobacillus acidophilus. After the culture media set, six wells per plate were done and the experimental material was inserted alternately with 1% gluconate chlorhexidine gel (positive control). The plates were maintained at room temperature during two hours for pre-diffusion of the material, and then incubated at 37°C for 24 hours in candlejars. After this period, the inhibition halo formed around the wells was measured with a digital calipter. The data analysis revealed that Papacárie® did not present antimicrobial properties for Streptococcus mutans and Lactobacillus acidophilus.
Keywords
Streptococcus mutans, Lactobacillus acidophilus, papain
References
1. Wolinsky LE. Caries and cariology. In: Nisengard RJ, Newman NG. Oral microbiology and immunology. Philadelphia: WB Saunders Company; 1994. p.341-359.
2. Ferreira CM, Bonifacio KC, Froner IC, Ito IY. Evaluation of the antimicrobial activity of three irrigating solutions in teeth with pulpal necrosis. Braz Dent J. 1999;10(1):15- 21.
3. Pitten FA, Kramer A. Antimicrobial efficacy of antiseptic mouthrinse solutions. Eur J Clin Pharmacol. 1999; 55: 95-100.
4. Rajashekhara E, Tippannavar CM, Sreenivasa NN, Sharma JS. Inhibitory activity of papain on facultative pathogens. Zentral Mikrobiol. 1990; 145: 455-6.
5. Russo A, Viotti PL, Vitali M, Clementi M. Antimicrobial activity of a new intact skin antisepsis formulation. Am J Infect Control. 2003; 31: 117-23.
6. Starley IF, Mohammed P, Schneider G, Bickler SW. The treatment of paediatric burns using topical papaya. Burns. 1999; 25: 636-9.
7. Flindt M. Health and safety aspects of working with enzymes. Process Biochem. 1978; 13 (8):3-7.
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