Avaliação dos Tecidos Periodontais de Dentes com Rizogênese Completa Autotransplantados para Alvéolos Recém-Preparados ou em Fase Inicial de Cicatrização. Estudo Histológico em Macacos.
Evaluation of the periodontal tissues of the mature teeth autotransplanted to either newly prepared or initial healing alveoli. Histologic study in monkeys
Bosco, A.F.; Saad Neto, M.; Nagata, M.J.H.; Pedrini, D.; Sundefeld, M.L.M.M.
Rev. odontol. UNESP, vol.29, n1-2, p.9-29, 2000
Resumo
O objetivo do presente trabalho foi analisar, comparativamente, o comportamento dos tecidos periodontais de dentes autotransplantados para alvéolos recém-preparados ou em fase inicial de cicatrização. Foram utilizados 3 macacos adultos (Cebus apella), com pesos aproximados de 1,8 kg. Foram transplantados 6 dentes imediatamente após o preparo do alvéolo (Grupo 1), e 6 dentes foram transplantados 10 dias após terem sido preparados os alvéolos (Grupo 2). Para cada grupo, em cada animal, um dente destinou-se para análise histológica aos 45 dias e, o outro dente, aos 90 dias após a realização dos autotransplantes dentais. Todos os dentes autotransplantados foram fixados ao dente vizinho com resina composta autopolimerizável por 7 dias, momento este em que tiveram seus canais instrumentados e preenchidos com pasta de hidróxido de cálcio. Nos dentes que se destinaram à análise histológica aos 90 dias após a realização dos transplantes, essa pasta foi substituída aos 45 dias. As peças de interesse, contendo o dente autotransplantado e seu periodonto, foram processadas em laboratório, sendo obtidos cortes seriados, com 6 micrômetros de espessura, transversais ao longo do eixo do dente, para análise histológica. Os resultados permitiram concluir que os dentes autotransplantados para alvéolos em fase inicial de reparo quando comparados com os autotransplantados para alvéolos recém-preparados: a) preservaram maior quantidade de ligamento periodontal no período final do experimento; b) apresentaram reparo dos tecidos periodontais de inserção sem anquilose e/ou reabsorção radicular por substituição; c) desenvolveram maior quantidade de reabsorção radicular de superfície, com pre-domínio do subtipo reparada.
Palavras-chave
transplante dental, transplante, transplante autólogo
Abstract
The aim of this study was to analyse, comparatively, the beha-viour of the periodontal tissues of the mature teeth autotransplantated to either newly prepared or initial healing alveoli. Three adult monkeys (Cebus apella) were used weighing approximatelly 1.8 kg. In each animal, 2 teeth were transplanted immediatelly after the alveoli prepare (Group 1) and 2 teeth were transplanted 10 days after the alveoli had been prepared (Group 2). For each group, one tooth was provided for histologic analysis on the 45th day and the other one on the 90th day after the teeth autotransplantation. All of the autotransplanted teeth were splinted to the next tooth using compo-site during 7 days. In the end of that period, they had their root canals instrumented and filled with calcium hydroxide paste. The teeth destinated to histologic analysis on the 90th day after the teeth transplantation had that paste replaced on the 45th day. The tissue blocks including the autotransplanted tooth and its periodontium were submitted to a routine laboratory procedure. The tissue blocks were sectioned perpendicular to the long axis of the teeth at a thickness of 6 μm. They were stained with hematoxilyn and eosin for the histologic analysis. The results permitted to conclude that comparatively to the autotransplanted teeth to the newly prepared alveoli, the autotransplanted teeth to the initial healing alveoli: a) preserved bigger amount of periodontal ligament in the end of the experimental period; b) presented repair of the periodontal attachment tissues without ankylosis and/or replacement root resorption; and c) developed bigger amount of surface root resorption, with dominance of the repaired type.
Keywords
Dental transplantation, transplantation, transplantation autologous
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