Hidroxiapatita microgranular: comportamento biológico em tecido subcutâneo de ratos
Histologic evaluation of tissue response to microparticles of hydroxyapatite subcutaneously implanted in rats
Oliveira, D.T.; Granjeiro, J.M.; Maeda, L.; Chacon, M.; Fonsêca, M.A.; Taga, E.M.; Taga, M.S.L.
Rev. odontol. UNESP, vol.22, n2, p.197-202, 1993
Resumo
Micropartículas ultrafinas de hidroxiapatita sintética foram implantadas na região subcutãnea de ratos. Os animais foram sacrificados nos intervalos de tempo de 3 e 24 horas e 7, 21, 30 e 60 dias após o implante, sendo os fragmentos teciduais incluídos em parafina e analisados pela microscopia óptica. A resposta tecidual induzida pela HA foi caracterizada, a partir do 72 dia, por uma inflamação crónica granulomatosa exuberante, com as células inflamatórias envolvendo as partículas. Neste trabalho não se observou nenhuma resposta imunológica exacerbada, o que reforça a biocompatibilidade do material.
Palavras-chave
Hidroxiapatitas, implantes artificiais, macrófagos
Abstract
Ultrafine microparticles of syntetic hydroxyapatite were implanted in the subcutaneous region ofrats. The animals were sacrificed at 3 and 24 hours, and 7, 21, 30 and 60 days post-operatively. The implants were formalin fixed, parafin embeddes and analysed through light microocopy. The tissue response induced by hydroxyapatite was characterized from 7th day by a exuberant foreign body reactions with the inflammatory cells surrounding hydroxyapatite particles. ln the present experiment, exacerbated immunologic response were not observed, confirming the biocompatibility of the material.
Keywords
Hydroxyapatites, implants, artificial, macrophages
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