Imunossupressão experimental em camundongos. Aspectos histológicos dos: órgãos linfóides após tratamento com dexametasona e inoculação de Carulida Albicans
Experimental immunosupression in mice. Histologycal aspects of the linfoid tissue after administration of dexamethasone and inoculation with Candida albicans
Brandão, A.A.H.
Rev. odontol. UNESP, vol.20, nÚnico, p.9-16, 1991
Abstract
Foi realizada imunossupressão experimental com corticosteróides em camundongos usando 1 mg/kg/dia de dexametasona durante uma semana. Para veríficar os efeitos imunossupressores da droga, sobre os tecidos linfóides, inoculamos 1 ml de 1.10^6 de Candida albicans, por via endovenosa, após o tratamento. Observamos a instalação e evolução da infecção durante doze dias e analisamos os aspectos histológicos do baço, timo, linfonodos, rins, fígado, pulmões e pele, que foram retirados, em intervalos regulares, de animais tratados somente com dexametasona e somente com Candida albicans, ou com ambos os dois tratamentos. Os resultados obtidos nos levaram a concluir que ocorre imunossupressão com essa dosagem de dexametasona e que o baço foi o órgão mais atingido. A candidíase isoladamente também provocou imunossupressão, afetando principalmente o timo. Os efeitos imunossupressores dos dois tratamentos se somaram quando houve a associação dos mesmos.
Keywords
Imunossupressão experimental, dexametasona, candidíase, órgãos linfóides
Resumo
Experimental corticosteroid immunosupression in mice was obtained using 1 mg/kg/day of dexamethasone during a week. In order to check the immunosupressive effects of this corticoid on the limphoid tissue we observed the animals endovenously with 1 mi of 1.10^6 of Candida albicans after the treatment with dexamethasone. We observed the set up and the development of the infection for 12 days and anatised the histologic aspects of the spects, thymus. lymph nodes, kidneys, Uver, lungs and skin obtained ar regular intervals from animals treated only with dexamethasone, only with Candida albicans or that had reeeived both treatments. From ou,. data we eoncluded that this dosage of dexamethasone induees immunosupression, affeeting speciaUy the spleen, that systemie eandidiasis is also able to induce immunosupression, affecting specially the thymus and that those immunosupressive effects are added when we associate the two treatments in mice.
Palavras-chave
Experimental immunosupression, dexamethasone, eandidiasis, linfoid tissue
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