Pesquisa auto-radiográfica sobre os efeitos de baixa dose de colchicina na dentinogênese do incisivo de rato
Autoradiographic investigation of the effects of low-dose colchicine on dentinogenesis in rat incisors
Nogueira, T.O.; Zorn, T.M.T.; Rossetini, S.M.; Koppang, H.S.
Rev. odontol. UNESP, vol.20, nÚnico, p.1-7, 1991
Resumo
Doze ratos receberam 0,5 mg/kg de colchicina (CLC) por via endovenosa. Cada três animais foram sacrificados após 5h, 24h, 3 dias e 7 dias após a administração de CLC. Noventa minutos antes do sacrifício, todos os animais receberam prolina triciada por via peritoneal. Os radioautogramas dos incisivos superiores foram submetidos a contagem e, posteriormente, foram analisados estatisticamente. Os resultados revelaram aumento na atividade secretora dos odontoblastos na porção mais incisal do dente, indicando um efeito estimulatório de CLC sobre a produção e secreção de colágeno após 3 dias de sua aplicação.
Abstract
Twelve wistar rats received 0.5 mg/kg colchicine (CLC) i.v. Three animals were sacrificed 5h, 24h, 3 days and 7 days after the injection. Ninety minutes before sacrifice all animals received tritiated proline intraperitoneally. Autoradiograms of sections from the maxillary incisor were subjected to quantitative, and statistical analysis. These results revealed an increased secretory activity in the odontoblasts in the more incisal parts of the tooth, indicating a stimulatory effect of CLC on collagen production and secretion after 3 days.
Keywords
Dentinogênese, cDentinogenesis, colchicine, collagenolchicina, colágeno
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