Revista de Odontologia da UNESP
Revista de Odontologia da UNESP
Original Article

Emprego da imunofluorescência direta no estudo das alterações de mucosa de palato, compatíveis com candidíase atrófica crônica, em indivíduos portadores de próteses dentárias totais muco-suportadas

Use of direct immunofluorescence in the study of the palate mucosa alterations, compatibles with chronic athrofic candidiases, in denture wearers

Cabral, L.A.G.

Downloads: 0
Views: 812


Estudo histopatológico com colorações pela hematoxilina-eosina (HE) e pelo ácido periódico de Schiff (PAS), paralelamente à imunofluorescência direta (ID) antiimunoglobulinas humanas totais e contra Candida albicans, foram efetuados em 25 indivíduos com próteses dentárias totais muco-suportadas superiores. Em 5 indivíduos sem sinais clínicos compatíveis com candidíase atrófica crônica (CAC) ao HE tivemos discreta inflamação no conjuntivo e o PAS marcou lâmina basal contínua, os grânulos intracelulares da camada granulosa e paraqueratina uniforme na superfície do epitélio. Nos 20 demais, com sinais clínicos compatíveis com CAC, ao HE foram frequentes achados comuns às infecções por Candida e o PAS revelou, além da descontinuidade da lâmina basal e superfície epitelial revestida por paraqueratina frouxa, ou descontfnua, a presença de corpúsculos arredondados maiores que os intracelulares da camada granulosa, por vezes isolados na porção mediana das papilas e cones interpapilares. Nos 5 primeiros indivíduos a ID antiimunoglobulinas humanas totais não revelou sinais da vigência de fenômenos imunológicos humorais, bem como nos demais 20 pacientes com sinais de CAC o método não mostrou qualquer vestígio de reação imunológica mediada por anticorpos. Porém, a ID com conjugado contra C. albicans nos casos com aspectos clínicos compatíveis com CAC detectou aspectos condizentes com estruturas que podem ser assumidas pela Candida nos tecidos. Culturas do material de indivíduos com sinais de CAC positivaram-se em 100% para Candida, 70% presuntivamente albicans; porquanto em indivíduos sem sinais de CAC, a Candidafoi isolada em 80% das amostras, 67% presuntivamente albicans.


Estomatites por dentadura, candidíases, imunofluorescência


Histopatology by hematoxilin-eosin (HE) and periodic acid Schilf (PAS), concomitant dírect immunofluorescence (DI) against total human immunoglobulins and against Candida albicans, was effectuated in 25 persons dentures wearers. ln 5 persons without chronic athrofic candidiasis (CAC) clinical signals in the palate the HE showed wise inflammatory elements in the connective tissue and the PAS marked the continuous basal layer, the intra-cellular grains of granular layer and the unifonn parakeratin on epithelial surface. ln 20 others, with palatal signals of CAC, in the HE was frequent the features encountered in Candida infected and PAS revealed, beside descontinuous lamina basal and epithelial surface corvered by tide and discontinuous parakeratin, the presence of round bodies few largers that presents in the granular layer, casually isolated in the medial portion of ret pegs and connective papillae. ln the first 5 persons the DI against total human immunoglobulins not showed signals of the humoral immunologic phenomena, the sarne was valid to others 20 patients with CAC clinical aspects. However the DI with conjugate against C. albicans in the 20 cases with CAC signals revealed suitable aspects of the structures assumed by Candida in tissues. Cultures of samples obtained of the persons with CAC signals was positive in 100% to Candida, 70% presumptively albicans, against 80% of positivness to generus Candida, 67% presumptively albicans, in the persons without CAC signals.


Denture stomatites, candidiasis, immunofluorescence


1. BERGENDAL, T. & HOLMBERG, K. - Studies of Candida sorology in denture stomatitis. Scand. J. dento Res., 90: 315-22, 1982.

2. BERGENDAL, T. & ISACSSON, G. A. - Combined clínical mycological, and histological study of denture stomatitis. Acta odont. scand., 41: 33-44,1983.

3. BERGMAN, B.; CARLSSON, G. E. & HEDEGARD, B. A. - Longitudinal two-year study of a number of full denture cases. Acta odont. scand., 22: 3-26, 1964.

4. BUDTZ-JORGENSEN, E. - Clínical aspects of Candida infection in denture wearers. J. amo dento Ass., 96: 474-9, 1978.

5. BUDTZ-JÓRGENSEN, E. - Denture stomatitis. III. Histopathology of trauma and Candida induced inflamatory lesions of the palatal mucosa. Acta odont. scand., 28:551-9, 1970.

6. BUDTZ-JÓRGENSEN, E. - Denture stomatitis. IV. An experimental model in monkeys. Acta odont. scand., 29: 513-26, 1971.

7. BUDTZ-JÓRGENSEN, E. - Denture stomatitis. V. Candida agglutinins in human sera. Acta odont. scand., 30: 313-25,1972.

8. BUDTZ-JÓRGENSEN, E. - Immune response to C. albicans in monkeys with experimental candidiasis in palate. Scand. J. dento Res., 81: 360-71,1973.

9. BUDTZ-JÓRGENSEN, E. - The significance of Candida albicans in denture stomatitis. Scand J. dento Res., 82: 151-90, 1974.

10. BUDTZ-JORGENSEN, E. & LÓE, H. - Chlorhexidine as a denture·desinfectant in the treatment of denture stomatitis. Scand. J. dento Res., 80: 457-64, 1972.

11. BUDTZ-JÓRGEMSEN, E.; STENDERUP, A. & GRABOWISKY, M. - An epidemiological study of yeasts in elderly wearers. Community dento oral Epidemiol., 3: 115-9, 1975.

12. CABRAL, L. A. G. - Verificação por imunofluorescência indireta da presença de anticorpos no soro e na saliva de humanos dirigidos contra Candida albicans. São Paulo, Faculdade de Odontologia, USP, 1980. (Tese - Mestrado)

13. CAHN, L. R. - The denture sore mouth. Ann. Dent., 3: 33-6,1936.

14. CALABRESI, P.; EDWARDS, E. A. & SCHILLING, R. F. - Fluorescent antiglobulin studies in leukopenic and related disorders. J. clin.lnvest., 38: 2091-9,1959.

15. CAWSON, R. A. - Chronic oral candidosis, denture stomatitis and hiperplasic candidosis. ln: Symposium on Candida infections. Annals. London, Livingstone, 1966. p. 139.

16. CAWSON, R. A. - Denture sore mouth and angular cheilitis. Brit. dento J., 115: 441-9, 1963.

17. CAWSON, R. A. - Thrushin adult out-patient. Dent. Practit. dento Rec., 15: 361-4, 1965.

18. COONS, A. H. & KAPLAN, M. H. - Localization of antigen in tissue cells. II. Improviments in a method for the detection of antigens by means of fluorescent antibody. J. exp. Med., 91: 1-13, 1950.

19. COONS, A. H.; GREECH, H.; JONES, R. N. & BERLINER, E. - The demonstration of pneumococcal antigens in tissue by the use of fluorescent antibody. J.lmmunol., 45: 159-70, 1942.

20. CRAIG, J. M. & FARBER, S. - The development of disseminated viceral mycosis during therapy for acute leukemia. Am. J. Pathol., 29: 601, 1953.

21. DAVENPORT, J. C. - The oral distribuition of Candida denture stomatitis. Br. dento J., 129: 151-6, 1970.

22. DIAMOND, R. D.; CLARK, R. A. & HAUDENSCHILD, C. C. - Damage to Candida albicans hyphae and pseudohyphae by myeIoparoxidase system and oxidation products of neutrophic metabolism "in vitro". J. din.Invest., 66: 909-17, 1980.

23. DIAMOND, R. D.; KRZESICKI, R. & JAO, W. - Damage to pseudohyphal forms of Candida albicans by netrophilis in the absence of serum "in vitro". J. dine Invest., 61: 349-59, 1978.

24. DOLON, W. C. - ImmunoIogy in dentistry. J. amo dento Ass.,100: 220-31,1980.

25. ENESTRÓM, S.; HED, J. & HUTMAN, P. - Detection of immune deposits in gIomeruli: a comparative study of paraffin embedded, enzime treated sections and cryostat sections as substrates in immunofluorescence. J. Immunol. Methods., 37: 343-51, 1980.

26. FISKER, A. V.; SCHÓTT, C. R. & PHILIPSEN, H. P. - Short term oral candidosis in rats, with special reference to the site of infection. Acta pathol. microbiol. scand., Sect. B, 90: 49-57, 1982.

27. GREENBERG, M. S. & COHEN, G. - Oral infection in immunossupressed renal transpIant patients. Oral Surg., 43: 879-85, 1977.

28. GORDON, M. A. - Dífferentation of yeast by means of fluorescent antibody. Proc. Soe. exp. Biol. Med., 97: 694-8, 1958.

29. GORDON, M. A.; ELLIOT, J. C. & HAWKINS, T. W. - Identification of Candida albicans, other Candida species and Torolopsis glabrata by means of immunofluerescent. Sabouraudia,5:323-8,1967.

30. HASENCLEVER, H. F. & MITCHELL, W. - Antigenic studies of Candida. L Observations of two antigenic groups in Candida albicans. J. Bacteriol., 82: 570-3, 1961.

31. HOWLETT, J. A. & SQUIER, C. A. - Candida albicans ultra-structures: colonizations and invasion of oral epithelium. Infect. immun. 29: 252-60, 1980.

32. JENPSEN, A. & WINTHER, J. E. - Mycotic infection in oralleukoplakia. Acta odont. scand., 23: 239-56, 1965.

33. JONES. J. H. & ADAMS, D. - Experimental induced acute oral Candidosis in rato Br.J. Dermatol., 83: 670-3, 1970.

34. KADE, H. & KAPLAN, L. - Evaluation of staining techniques in the histologic diagnosis of fungi. Arch. Pathol., 59: 571-7, 1955.

35. KAPICA, L. & BLANK, F. - Grow of Candida albicans on Keratin as sole source of nitrogen. Dermatologica (Basel),115: 81-105,1957.

36. KEMP, G. & SOLOROTOVSKY, M. - FIuorescent antibody studie of pathogenesis in experimental Candida albicans infections of mÍce. J.Immunol., 88: 777-81, 1962.

37. KEYE, J. D. & MaGEE, W. E. - Fungal diseases in a general hospital, a study of 88 patients. Am. J. dine Pathol., 26: 1235-53, 1956.

38. KNIGHT, L. & FLETCHER, 1. - Growth of Candida albicans in saliva: stimulation by glucose associated with antibiotics, corticosteroids, and diabetes mellitus. J. infecto Dis., 123: 371-7, 1971.

39. KOBAYASHI, G.; FRIEDMAN, L. & KOFROTH, J. - Some cytological and pathogenic properties of Candida albicans. J. Bacteriol., 88: 795-801, 1964.

40. LEHNER, T. - Immunofluorescent inv~stigation of Candida albicans antibodies in human saliva. Arch. oral Biol., 10: 975-80, 1965.

41. LEHNER, T. - Immunofluorescent study of Candida albicans candidiasis, carriers and controIs. J. Pathol. Bacteriol., 91: 97-104, 1966.

42. LEHNER, T.-Oralcandidosis. Dent.Practit., 17:209-16,1967.

43. LEHNER, T. Symposium on denture sore mouth. III. Immunofluorescent investigation of Candida. Dent. Practit., 16: 142-5, 1965.

44. LEHNER, R. I. & CLINE, M. J. - Interaction of Candida atbicans with human leukocytes and serum. J. Bacteriol., 98: 996-1004, 1969.

45. LEHNER, R. L; LADRA, K. M. & HAKE, R. B. - Nonoxidative fungicidal mechenisms of mammalian granulocytes, demonstration of components with candidacial activity in human, rabbit, and guinea pig leukocites. Infect.Imun., 11: 1226-34, 1975.

46. LOPEZ-MARTINEZ, R. - Caracteristicas imunológicas en candidosis. Prensa medo mex., 39: 468-71, 1974.

47. MAIBACK, H. I. & KLIGMAN, A. M. - The biology of experimental human cutaneos moniliasis. Arch. Dermatol., 85: 233-56, 1962.

48. MAJEESKY, S. & MACURA, A. B. - The incidence of yeast lihe fungi in denture wearers oral cavities. Mykosen, 23: 516-24, 1980.

49. MONTIES, L. F. & WILBORN, W. H. - Ultrastructural features of host-parasite relationship in oral candidiasis. J. Bacteriol., 96: 1349-56, 1968.

50. NAIRN, R. I. - Nystatin and amphotericin C in the treatment of denture relates candidiasis. Oral Surg., 40: 68-75, 1975.

51. NEWTON, A. V. - Denture sore mouth, a possible aetiology. Br. dento J., 112: 357-60, 1962.

52. NYQUIST, G. - A study of denture sore mouth: an investigation of the oral mucosa arising from the use of full dentures. Acta odont. scand., 10 (Suppl. 9): 1-123, 1952.

53. OLSEN, I. & BIRKELAND, J. M. - Denture stomatitis: yeast occurrence and the pH of saliva and denture plaque. Scand. J. dento Res., 85: 130-4, 1977.

54. PHILIPSEN, H. P.; CLEATON-JONES, P. & FISKER, A. - Correlative light microscopic and scanning completely and incompletely orthokeratinized rat oral epithelium. Scand. J. dento Res., 90: 255-62, 1982.

55. POULTER, R.; JEFFERY, K.; HUBBARD, M. J.; SHEPHERD, M. G. & SULLIVAN, P. A. - Parasexual genetic analysis of Candida albicans by spheroplast fusion. J. Bacteriol., 146: 833-40, 1981.

56. PRYOR, W. J. - Antiseptics for use in the mouth of denture patients. Appolonia,ll: 147-62, 1936.

57. RITCHIE, G. M.; FLETCHER, A. M.; MAIN, D. M. G. & PROPHET, A. S. - The etiology exfoliative cytology and treatment of denture stomatitis. J. prosth. Dent., 22: 185-200, 1969.

58. SAINT-MARIE, G. A. - A paraffm embedding technich for studies employing immunofluorescence. J. Histochem. Cytochem., 10: 550-7, 1962.

59. SHAKIR, B. S.; MARTIN, M. V. & SMITH, C. J. - Induced palatal candidiosis i the wistar rato Arch. oral Biol., 26: 787-93, 1981.

60. SHEPHERD, M. G. - Candidiasis: an infectious disease of increasing importance. N. Z. dento J., 78: 89-93, 1982.

61. STALLYBRASS, F. C. - Candida precipitins. J. Pathol. Bacteriol., 87: 89-97, 1964.

62. SUMMERS, D. F.; GROLLMAN, A. P. & HASENCLEVER, H. F. - Polysaccharide antigensofcandidacell was. J.Immunol., 92:491-9,1964.

63. SVIHLA, G.; SCHLENK, F. & DAINKO, J. L. - Spheroplasts of yeast Candida utilis. J. Bacteriol., 82: 808-14, 1961.
588017507f8c9d0a098b467e rou Articles
Links & Downloads

Rev. odontol. UNESP

Share this page
Page Sections