Revista de Odontologia da UNESP
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Revista de Odontologia da UNESP
Original Article

Emprego da imunofluorescência direta no estudo das alterações de mucosa de palato, compatíveis com candidíase atrófica crônica, em indivíduos portadores de próteses dentárias totais muco-suportadas

Use of direct immunofluorescence in the study of the palate mucosa alterations, compatibles with chronic athrofic candidiases, in denture wearers

Cabral, L.A.G.

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Resumo

Estudo histopatológico com colorações pela hematoxilina-eosina (HE) e pelo ácido periódico de Schiff (PAS), paralelamente à imunofluorescência direta (ID) antiimunoglobulinas humanas totais e contra Candida albicans, foram efetuados em 25 indivíduos com próteses dentárias totais muco-suportadas superiores. Em 5 indivíduos sem sinais clínicos compatíveis com candidíase atrófica crônica (CAC) ao HE tivemos discreta inflamação no conjuntivo e o PAS marcou lâmina basal contínua, os grânulos intracelulares da camada granulosa e paraqueratina uniforme na superfície do epitélio. Nos 20 demais, com sinais clínicos compatíveis com CAC, ao HE foram frequentes achados comuns às infecções por Candida e o PAS revelou, além da descontinuidade da lâmina basal e superfície epitelial revestida por paraqueratina frouxa, ou descontfnua, a presença de corpúsculos arredondados maiores que os intracelulares da camada granulosa, por vezes isolados na porção mediana das papilas e cones interpapilares. Nos 5 primeiros indivíduos a ID antiimunoglobulinas humanas totais não revelou sinais da vigência de fenômenos imunológicos humorais, bem como nos demais 20 pacientes com sinais de CAC o método não mostrou qualquer vestígio de reação imunológica mediada por anticorpos. Porém, a ID com conjugado contra C. albicans nos casos com aspectos clínicos compatíveis com CAC detectou aspectos condizentes com estruturas que podem ser assumidas pela Candida nos tecidos. Culturas do material de indivíduos com sinais de CAC positivaram-se em 100% para Candida, 70% presuntivamente albicans; porquanto em indivíduos sem sinais de CAC, a Candidafoi isolada em 80% das amostras, 67% presuntivamente albicans.

Palavras-chave

Estomatites por dentadura, candidíases, imunofluorescência

Abstract

Histopatology by hematoxilin-eosin (HE) and periodic acid Schilf (PAS), concomitant dírect immunofluorescence (DI) against total human immunoglobulins and against Candida albicans, was effectuated in 25 persons dentures wearers. ln 5 persons without chronic athrofic candidiasis (CAC) clinical signals in the palate the HE showed wise inflammatory elements in the connective tissue and the PAS marked the continuous basal layer, the intra-cellular grains of granular layer and the unifonn parakeratin on epithelial surface. ln 20 others, with palatal signals of CAC, in the HE was frequent the features encountered in Candida infected and PAS revealed, beside descontinuous lamina basal and epithelial surface corvered by tide and discontinuous parakeratin, the presence of round bodies few largers that presents in the granular layer, casually isolated in the medial portion of ret pegs and connective papillae. ln the first 5 persons the DI against total human immunoglobulins not showed signals of the humoral immunologic phenomena, the sarne was valid to others 20 patients with CAC clinical aspects. However the DI with conjugate against C. albicans in the 20 cases with CAC signals revealed suitable aspects of the structures assumed by Candida in tissues. Cultures of samples obtained of the persons with CAC signals was positive in 100% to Candida, 70% presumptively albicans, against 80% of positivness to generus Candida, 67% presumptively albicans, in the persons without CAC signals.

Keywords

Denture stomatites, candidiasis, immunofluorescence

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