Fatores enzimáticos de agressão produzidos por bactérias aeróbicas da cavidade bucal
Production of histolytic enzymes by aerobic oral bacteria
Shimizu, M.T.; Almeida, N.Q.
Rev. odontol. UNESP, vol.18, nÚnico, p.199-204, 1989
Resumo
Verificou-se a produção de hialuronidase, condroitin sulfatase, lecitinase e gelatinase em diferentes amostras bacterianas, isoladas da cavidade bucal. Hialuronidase foi produzida por 20 das 30 amostras de S. aureus, 18 das 21 amostras de S. pyogenes e todas as 4 amostras de A. viscosus. Nenhuma amostra produziu condroitin sulfatase e gelatinase; todas as 30 amostras de S. aureus e todas as amostras de A. viscosus produziram lecitinase.
Palavras-chave
Enzimas histolíticas, bactérias da cavidade bucal, hialuronidase, condroitin sulfatase, lecitinase, gelatinase
Abstract
The production of hyaluronidase, chondroitin sulfatase, lecithinase and gelatinase was investigated in various bacterial strains isolated from the oral cavity. Hyaluronidase was found to be produced by 20 of the 30 strains of the S. aureus, 18 from the 21 strain of the S. pyogenes and all the 4 strains of the A. viscosus. None of the strain searched produced chondroitin sulfatase and gelatinase. All the 30 strains of the S. aureus and all the strains of the A. viscosus produced lecithinase.
Keywords
Histolytic enzymes, aerobic oral bacteria, hyaluronidase, chondroitin sulfatase, lecithinase, gelatinase.
References
1. AISENBERG, M. S. & AISENBERG, A. D. - Hyaluronidase in periodontal disease. Oral Surg., 4: 317-20, 1951.
2. BENCHETRIT, L. C.; AVELINO, C. C. & OLIVEIRA, C. M. - Serotypes and extra cellular hyaluronidase of group A Streptococc:i. Rev. Microbiol, 15: 35-8, 1984.
3. BRAUDE, A. I. -Microbialogy. Philadelphia, W. B. Saunders, 1982. 845p.
4. BURNETI, G. W.; SCHERP, H. W. & SCHUSTER, G. S. - Microbiologia Oral & Doenças Infecciosas. 3. ed. Rio de Janeiro, Guanabara Koogan, 1978. 765p.
5. CIMASONI, G. & HELD, A. J. - Intracellulal' mucopolysacharides in human gingival epithelium. Arch. oral Bial.,9: 751-2, 1964.
6. CRAWLEY, N. - Hyaluronidase production by hemolitic streptococci of human origino J. Pathol. Bact., 56: 27-35, 1944.
7. DAHLÉN, G.; WIKSTROM, M. & MOLLER, A. - Production of histolytic enzymes by a combination of oral bacteria with know pa.thogenicity. J. dento Res., 62: 1041-4, 1983.
8. EVANS, D. G. & WARDLAW, A. C. - Gelatinase and collagenase production by certain species of Bacillus. J. Gen. Microbiol., 8: 481-7, 1953.
9. GAFFER, A.; COLEMAN, E. J. & MARCUSEN, H. W. - Penetration of dental plaque components intra gingiva: sequential topkal treatments with hyaluronidase and streptococcal polysaccharides in rats. J. Penodontol., 52: 197-205, 1981.
10. GILLESPIE, W. A. & ALDER, V. G. - Production of opacity in egg-yolk by coagulase-positive staphylococci. J. Path. Bact., 64: 187, 1952.
11. HERSHON, L. E. ~ Elaboration of hyaluronidase and chondroitin sulfatase by microrganisms inhabiting the gingival sulcus: evaluation of a screening method for periodontal disease. J. Periodontol., 42: 34-6, 1971. .
12. HOEFFLER, U. - Enzymatic and hemolytic properties of Propionibacteri'um acnes and related bacteria. J. clin. Microbial. 6: 555-8, 1977.
13. -HOFFLER, U. - Production of hyaluronidase by propionibacteria from different origins. Zbl. Bakt. Hyg., I. Abt. Orig. A, 245: 123-9, 1979.
14. KJELLMAN, O.; LINDER, L. & FROSTELL, G. - Effect in vitro of pH and low glucose concentrations on the growth and hyaluronidase activity of a strain of Streptococcus mitis. Odont. Revy, 22: 27-36, 1971.
15. LEDERMAN, H. M.; HAYASCHI, J. A. & BAHN, A. N.-Chondroitin sulfatase in oral. J. dento Res:, 51: 206, 1972.
16. LEUNG, S. W. - The relation of calculus, plaque and food impaction to periodontal disease. J. dento Res., 41: 306-11, 1962.
17. MAC DONALD, J. B.; GIBBONS, R. J. & SOCRANSKY, S. S. - Bacterial mechanisms in periodontal disease. Ann. N. Y. Acad. Sd., 85: 467, 1960.
18. MAHLER, I. R. & LISANTI, V. F. - Hyaluronidase production microrganisms from human saliva. Oral Surg., 5: 1235-40, 1952.
19. NOLTE, W. A. - Oral microbiology with basic microbiology and immunology. 4. ed. London, C. V. Mosby CO., 1982. 795p.
20. PAIVA, G. M. & ITO, I. I. - Eficiência da prova da lecitinase com 100 amostras relativamente às provas de coagulase fermentação do manitol e DNAse, na diferenciação entre "Staphylococcus aureus" e "s epidermidis". Rev. bras. Anál. din., 4: 13, 1972.
21. PIKE, R. - The production of hyalirronic acid and hyaluronidase some strains of g~:oup A streptococci. Ann. N. Y. Acad. Sei., 52: 1070-3, 1950.
22. PUHVEL, S. M. & REISNER, R. M. - Theproduction of hyaluronidase (Hyaluronate lyase) by Corynebacterium acnes. J.Invest. Dermatol., 58: 66-70, 1972.
23. ROSAN, B. & WILLIAMS, N. B. - Hyaluronidase production by oral enterococci. Arch. oral Biol., 9: 291-8, 1964.
24. ROSAN, B. & WILLIAMS, N. B. - Hyaluronidase production by oral enterococci. Arch. oral Biol., 9: 291-8,1964.
25. SCHERP, H. W. - Discussion of bacterial factors in periodontal disease. J. dento Res., 41: 327-30, 1962.
26. SCHULTZ-HAUDT, S. D. & SCHERP, R. W. - Production of hyaluronidase and beta-glucorindase by viridans streptococci isolated from gingival creavices. J. dento Res., 34: 924-9, 1955.
27. SMITH, R. f. & WILLETT, N. P. - Rapid plate method for screening hyaluronidase and chondroitin sulfatase - producing microorganisms. Appl. Microbiol., 16: 1434-6, 1968.
28. STEFEN, E. K. & HENTGES, D. I. - Hydrolytic enzymes of anaerobic bacteria isolated from human infections. J. dine Microbiol., 14: 153-6, 1981.
29. TAKEUCHI, H.; HORI,Y., KANEHISA, J.; TANI, A.; TAKAI, R. & SAGAWA, H.Bacterial chondroitinase ABC and hyaluronidase in human dental plaque and inflamed gingiva. J. Osaka dento Univ., 16: 183-7, 1982.
30. TAM, Y. C. & CHAN, E. C. S. - Phase variation of hyaluronidase producing peptoestreptococci associated with periodontal disease. J. dento Res., 62: 1009-12, 1983.
31. TAM, Y. C.; HARVEY, R. f. & CHAN, E. C. S. - Chondroitin sulfatase producing and hyaluronidase producing oral bacteria associated with periodontal disease. J. Canad. Dent. Ass., 115-20, 1982.
32. THILANDER, H. - The effect of leococytic enzymes activity on the structure of the gingival pocket epithelium in mano Acta. odont. Scand., 21: 431-51, 1963.
33. UZEDA, M. - Mucopolissarises (Hialuronidase e Condrosulfatase) em componentes da microbiota do sulco gengival humano. Rio de Janeiro, Instituto de Microbiologia, 1973. 88p. (Tese de Mestrado)
34. WILLIS, A. T. - The lipolytic activity af some clostridia. J. Path. Bact., 80: 79, 1960.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email