Estudo citoquímico do complexo de golgi em fibroblastos do tecido conjuntivo de rato
Cytochemical study of the Golgi complex compartimentalization on fibroblastos of the rat foot pad
Marchi, F.
Rev. odontol. UNESP, vol.15, nÚnico, p.83-89, 1986
Resumo
Foi realizado o estudo citoquímico ao nível de microscopia eletrônica, em fibroblastos do tecido conjuntivo de rato, após a impregnação prolongada com tetróxido de ósmio e pela detecção da enzima tiaminopirofosfatase. Os dois sáculos da face cis da pilha do Golgi, com as suas porções centrais achatadas e as porções periféricas dilatadas ficaram impregnados pelo tetróxido de ósmio, enquanto o produto de reação para detecção da enzima TPPase foi observado nos sáculos da região trans. Tais resultados comprovam a nítida compartimentalização do complexo de Golgi em fibroblastos do tecido conjuntivo de rato.
Palavras-chave
Citoquímica, complexo de Golgi, fibroblasto
Abstract
The Golgi complex of fibroblasts from the rat foot pad was characterized using cytochemical methods. After prolongued impregnation with osmium tetroxide black deposits were seen over the two first saccules (cis face) of the stack, including their straight and dilated portions. TPPase reaction products were seen mainly over the straight portion of the 4 th saccule (trans face). This study added further evidence ofthe compartimentalization of the Golgi complex in collagen-producing fibroblast.
Keywords
Cytochemical study, Golgi complex, fibroblast
References
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