Revista de Odontologia da UNESP
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Revista de Odontologia da UNESP
Original Article

Estudo radioautográfico da síntese de dna do epitélio gengival de camundongos Mus musculus I. Influência da idade

Radioautographic DNA synthesis study on mice Mus Musculus gingival epithelium. I. Age effeet

Tarelho, Z.V.S.; Hetem, S.

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Resumo

Foi estudada a frequência de células que sintetizam DNA na camada basal do epitélio gengival da região do primeiro molar inferior de camundongos jovens e adultos. Para isso, usou-se timidina triciada e técnica radioautográfica. A freqüência de células marcadas foi determinada através do estabelecimento de sua proporção na camada basal do epitélio gengival. Os valores obtidos foram submetidos à análise estatística, e foi verificado que a freqüência de células marcadas aumenta com a idade nos dois sexos.

Palavras-chave

Síntese de DNA, radioautografia, timidina triciada, epitélio gengival, idade

Abstract

It was studied the DNA-synthetizing cells frequency in the gingivol epithelium bosal layer of the first lower molar region in young adult mice during, 3H-thymidine and radioautography. The labeled cells frequency was determined by calculating, their proportions. The data were statiscally analysed ond it was determined that the market cells frequency increases with age in both sexes.

Keywords

DNA synthesis, radioautography, 3H-thymidine, gingival epithelium, age

References



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