Revista de Odontologia da UNESP
https://revodontolunesp.com.br/journal/rou/article/doi/10.1590/1807-2577.1037
Revista de Odontologia da UNESP
Original Article

Efeitos da dentina sobre o pH e atividade antimicrobiana de diversas formulações com hidróxido de cálcio

Effects of dentin on the pH and antimicrobial activity of several calcium hydroxide formulations

Galoza, M. O. G.; Kuga, Milton Carlos; Silva, Daniele Fockink da; Weckwerth, Paulo Henrique; Keine, Kátia Cristina; Pereira, Jefferson Ricardo; Andrade, Marcelo Ferrarezi de

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Resumo

Objetivo: Avaliar o efeito da dentina sobre o pH e a atividade antimicrobiana de formulações com hidróxido de cálcio. Material e método: O pH e a atividade antimicrobiana das formulações (G1- polietilenoglicol, G2- glicerina e paramonoclorofenol canforado ou G3- clorexidina) foram analisados nas formulações puras (G1A, G2A e G3A) ou acrescidas de dentina em pó (G1B, G2B e G3B). Tubos de polietileno foram preenchidos com uma das formulações e imersos em água destilada. O pH foi analisado após 24 horas, 7, 14 e 21 dias. A atividade antimicrobiana foi avaliada sobre Enterococcus faecalis (ATCC 29212), através do teste de difusão em ágar. Os resultados foram submetidos aos testes de ANOVA e Tukey (p=0,05). Resultado: A dentina não exerceu efeito sobre o pH das formulações, exceto ocasionando a redução em G1, no período de 24 horas (p<0,05). Nas demais formulações, não houve interferência da dentina sobre o pH (p>0,05). Quando comparados entre os grupos, em 24 horas e 7 dias, G1A e G1B apresentaram menor pH (p<0,05). Em 14 dias, G2A, G2B, G3A e G3B demonstraram similar pH (p>0,05), assim como entre G3B e G1A (p>0,05). Em 21 dias, todas as formulações demonstram semelhante pH (p>0,05). Os resultados em ordem decrescente da atividade antimicrobiana foram G2A=G2B>G3A=G3B>G1A=G1B (p<0,05). Conclusão: A dentina não interferiu no pH e na atividade antimicrobiana das diferentes formulações com hidróxido de cálcio, exceto na associação com o polietilenoglicol, em que ocasionou a redução do pH apenas no período de 24 horas. A formulação com paramonoclorofenol canforado apresentou maior atividade antimicrobiana, seguida da clorexidina e do polietilenoglicol.

Palavras-chave

Cálcio, endodontia, Enterococcus faecalis, pH.

Abstract

Objective: To evaluate the dentin effect on pH and antimicrobial activity of the calcium hydroxide chemical formulations. Material and method: pH and antimicrobial activity of calcium hydroxide formulations were evaluated (G1-polyethyleneglycol, G2-glycerin and camphorated paramonochlorophenol and G3-chlorhexidine). The formulations were evaluated alone (G1A, G2A e G3A) or with dentin powder (G1B, G2B and G3B). Polyethylene tubes were filled with the formulation and immersed in distilled water. After 24 hours, 7, 14 and 21 days, pH was evaluated. The antimicrobial activity was evaluated against Enterococcus faecalis by agar diffusion test. All data were submitted to ANOVA and Tukey tests (p=0.05). Result: None effect was observed on pH and antimicrobial activity by dentin on different chemical formulations, except the reduction occurred in G1 at 24 hours (p <0.05). When compared between the groups at 24 hours and 7 days, G1A and G1B had a lower pH (p <0.05). At 14 days, G2A, G2B, G3A and G3B showed similar pH (p <0.05) and between G3B and G1A (p> 0.05). At 21 days, all formulations were similar (p> 0.05). Regarding antimicrobial activity G2B G2A => = G3A G3B> = G1A G1B (p <0.05). Conclusion: The dentin did not affect the pH and antimicrobial activity of different formulations containing calcium hydroxide, except in association with polyethylene glycol, which caused the reduction of pH, within 24 hours. The formulation with paramonochlorophenol camphor showed higher antimicrobial activity, followed by chlorhexidine and polyethylene glycol.

Keywords

Calcium, endodontic, Enterococcus faecalis, pH.

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