Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.26115
Revista de Odontologia da UNESP
Original Article

Estudo comparativo entre a produção de fosfolipases extracelulares e proteinases do gênero Candida isoladas a partir de infecções de cavidade oral

Comparative study between extracellular phospholipase and proteinase production in clinically important of the genus Candida isolated in oral candidiasis patients

Andreola, Patrícia; Demathé, Adriana; Galafassi, Daniel; Elsemann, Estelamari Barbieri; Elsemann, Rogério Brasiliense; Gazzoni, Alexandra Flávia

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Resumo

Introdução: A habilidade da Candida spp. em produzir enzimas proteolíticas, tais como fosfolipase e proteinases, tem um papel importante na patogenicidade destas leveduras. Objetivo: Determinar as espécies causadoras das infecções orais por Candida spp., além de investigar a atividade in vitro das fosfolipases e proteinases em isolados clínicos do gênero Candida, provenientes de pacientes com candidíase oral. Material e método: Isolados de Candida spp., pertencentes à Coleção de Cultivos Fúngicos do Laboratório de Microbiologia e Patologia Oral do Departamento de Odontologia da Faculdade da Serra Gaúcha, foram analisados. Produção de fosfolipases foi analisada utilizando-se Ágar gema de ovo. Liberação de proteinases foi medida utilizando-se extrato de levedura adicionado à albumina bovina. Resultado: Um total de 35 isolados clínicos do gênero Candida foi testado. C. albicans foi a espécie predominante (77%). Os demais isolados identificados foram: C. parapsilosis (20%) e C. tropicalis (2%). Ao comparar a atividade de fosfolipase do grupo C. albicans com o grupo Candida não-albicans, foi encontrada diferença significativa (P=0,04). Não foi encontrada diferença significativa entre a C. albicans e a C. não-albicans, para a produção de proteinase. A liberação de proteinase foi significativamente maior quando comparada à produção de fosfolipase para o gênero Candida (P=0,04). Diferença estatisticamente significativa foi encontrada quando a atividade de fosfolipase e proteinase da C. albicans foi comparada à atividade das espécies de C. não-albicans (P=0,02). Conclusão: Diferentes quantificações de fosfolipase extracelular e atividade de proteinase têm sido atribuídas aos isolados clínicos de C. albicans quando comparados a outras espécies de Candida.

Palavras-chave

Enzimas extracelulares, Candida spp., fatores virulência.

Abstract

Introduction: The ability of the genus Candida to produce secretory enzimes such as proteinase and phospholipases to play an important role in the patogenicity of these yeasts. Objective: To determine the Candida species isolates from oral cavity infections and to investigate in vitro phospholipase and protease activities in clinically important of the genus Candida isolated in oral candidiasis patients. Material and method: Candida species isolated of the Fungal Culture Collection of Oral Microbiology and Pathology Testing Service Laboratory of the Dentistry Departament of Faculdade da Serra Gaúcha were evaluated. The phospholipases detection was assayed using the egg yolk agar plate method. Determination of protease production was performed in agar plates containing bovine serum albumine and yeasts extract. Result: A total of 35 isolates of the genus Candida were tested. C. albicans was the species predominant (77% of isolates), followed by C. parapsilosis (20%) and C. tropicalis (2%). When compared the phospholipase activity of the C. albicans group with the non-albicans Candida species types group was observed significant difference among of this groups (P=0.04). No statiscally significant difference between the C.albicans and non-albicans Candida species types when was compared to proteinase production. Proteinase production was higher and statiscally significant when compared to phospholipase activity in the genus Candida isolates (P=0.04). Statiscally significant differences were found between phosphoplipases and proteases activity for C. albicans when compared to non-albicans Candida species types (P=0.02). Conclusion: Differences in the secretion rates of phospholipase extracellular and proteases activity have been attributed to clinical strains of C. albicans when compared to others Candida species types.

Keywords

Extracellular enzymes, Candida spp., virulence factors.

References

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