Revista de Odontologia da UNESP
ISSN 1807-2577 (Eletrônico)
Artigo Original

Application of forensic luminol for blood detection in endodontic files

Aplicação do luminol forense para detecção de sangue em limas endodônticas

Rodrigo Arruda-vasconcelos, Letícia Gomes Ferreira Chantre, Rosangela Sabbatini Capella Lopes, Cláudio Cerqueira Lopes, Marlos Barbosa-ribeiro, Brenda Paula Figueiredo de Almeida Gomes

Abstract

Introduction: Blood is a biological material with high potential of infectious transmission in dental environments, including herpes simplex, hepatitis and AIDS. Aim: To investigate the efficacy of luminol in detecting blood in endodontic files before and after the sterilization process. Material and method: Luminol was used to investigate the presence or absence of traces of blood tissue in 50 endodontic files, visible to naked eye or not, after performing endodontic treatment and after the cleaning/sterilization process. The results obtained were tabulated and statistically analyzed by using the Friedman’s test at a significance level of 5% (p<0.05). Result: By naked eye, it was found that 31/50 files showed no trace of blood, 8/50 showed a slight presence of blood and 11/50 showed a considerable presence of blood after endodontic treatment. After the use of luminol, however, 16/50 endodontic files showed no trace of blood, 19/50 showed a slight presence of blood and 15/50 showed a considerable presence of blood. After the cleaning and sterilization process, no blood was detected in the files. Conclusion: It was concluded that the luminol solution is effective in detecting blood tissue in endodontic files as well as in validating the cleaning/sterilization process.

Keywords

Endodontic file; endodontic therapy; endodontics; infection control.

Resumo

Introdução: Sangue é um material biológico com alto potencial de transmissão de infecção em ambientes odontológicos, incluindo herpes simples, hepatites e AIDS. Objetivo: Investigar a eficácia do luminol em detector sangue em limas endodônticas antes e após o processo de esterilização. Material e método: Luminol foi utilizado para investigar a presença ou ausência de vestígios tecido sanguíneo em 50 limas endodônticas, visíveis ou não à olho nu, após a realização do tratamento endodôntico e após o processo de limpeza/esterilização. Os resultados obtidos foram tabulados e analisados estatisticamente utilizando o teste de Friedman com nível de significância de 5% (p<0,05). Resultado: A olho nú, foi observado que 31/50 limas não apresentaram vestígios de sangue, 8/50 apresentaram uma leve presença de sangue e 11/50 apresentaram uma presença considerável de sangue após o tratamento endodôntico. Após a utilização do luminol, entretanto, 16/50 limas endodônticas não apresentaram vestígios de sangue, 19/50 apresentaram uma leve presença de sangue e 15/50 apresentaram uma presença considerável de sangue. Após o processo de limpeza e esterilização não foi detectado sangue nas limas endodônticas. Conclusão: A solução de luminol é efetiva na detecção de tecido sanguíneo em limas endodônticas, validando o processo de limpeza/esterilização.

Palavras-chave

Lima endodôntica; terapia endodôntica; endodontia; controle de infecção.

Referências

1. Bergervoet PW, van Riessen N, Sebens FW, van der Zwet WC. Application of the forensic luminol for blood in infection control. J Hosp Infect. 2008 Apr;68(4):329-33. PMid:18346814. http://dx.doi.org/10.1016/j.jhin.2008.01.026.

2. Shah R, CollinsJM, Hodge TM, Laing ER. A national study of cross infection control: “are we clean enough? Br Dent J. 2009 Sep;207(6):267-74. PMid:19779516. http://dx.doi.org/10.1038/sj.bdj.2009.824.

3. Cohen AS, Jacobsen EL, BeGole EA. National survey of endodontists and selected patient samples: infectious diseases and attitudes toward infection control. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997 Jun;83(6):696-702. PMid:9195626. http://dx.doi.org/10.1016/S1079-2104(97)90322-X.

4. Terezhalmy GT, Gitto CA. Today’s minimal requirements for a practical dental office infection control and exposure control program. Dent Clin North Am. 1998 Oct;42(4):629-42. PMid:9891645.

5. Yüzbasioglu E, Saraç D, Canbaz S, Saraç YS, Cengiz S. A survey of cross-infection control procedures: knowledge and attitudes of Turkish dentists. J Appl Oral Sci. 2009 Dec;17(6):565-9. PMid:20027427. http://dx.doi.org/10.1590/S1678-77572009000600005.

6. Cottone JA, Molinari JA. State-of-the-art infection control in dentistry. J Am Dent Assoc. 1991 Aug;122(8):33-41. PMid:1918684. http://dx.doi.org/10.14219/jada.archive.1991.0254.

7. Miller CH. Cleaning, sterilization and disinfection: basics of microbial killing for infection control. J Am Dent Assoc. 1993 Jan;124(1):48-56. PMid:8383152. http://dx.doi.org/10.14219/jada.archive.1993.0022.

8. Lopes CC, Lopes RSC, Cardoso JN, da Silva JA, Silva JÁ, Ferreira LG. Hydrazines and derivatives production process from hydrazines and dicarboxylic acid. US 7517983 B2. 2009 April 4.

9. Touwen E. A comparative study of chemical detection reagents for reagents for latent bloodstains [dissertation]. Amsterdam: Netherlands Forensic Institute, University of Amsterdam; 2013.

10. Barni F, Lewis S, Berti A, Miskelly GM, Lago G. Forensic application of the Luminol reaction as a presumptive test for latent blood detection. Talanta. 2007 May;72(3):896-913. PMid:19071703. http://dx.doi.org/10.1016/j.talanta.2006.12.045.

11. Gross AM, Harris KA, Kaldun L. The effect of luminol on presumptive tests and DNA analysis using the polymerase chain reaction. J Forensic Sci. 1999 Jul;44(4):837-40. PMid:10432617. http://dx.doi.org/10.1520/JFS14561J.

12. Webb JL, Creamer JI, Quickenden TI. A comparison of the presumptive luminol test for blood with four non-chemiluminiscent forensic techniques. Luminescence. 2006 Jul-Aug;21(4):214-20. PMid:16645959. http://dx.doi.org/10.1002/bio.908.

13. Kotze MJ, Labuschagne W. A method of determining the presence of blood in and on a dental needle after the administration of local anesthetic. J Am Dent Assoc. 2014 Jun;145(6):557-62. PMid:24878710. http://dx.doi.org/10.14219/jada.2014.14.

14. McColl E, Bagg J, Winning S. The detection of blood on dental surgery surfaces and equipment following dental hygiene treatment. Br Dent J. 1994 Jan;176(2):65-7. PMid:8117477. http://dx.doi.org/10.1038/sj.bdj.4808365.

15. Asman B, Engström PE, Olsson T, Bergström K. Increased luminol enhanced chemiluminescence from peripheral granulocytes in juvenile periodontitis. Scand J Dent Res. 1984 Jun;92(3):218-23. PMid:6589737.

16. Ramadan AA. Removing hepatitis C virus from polytetrafluoroethylene-coated orthodontic archwires and other dental instruments. East Mediterr Health J. 2003 May;9(3):274-8. PMid:15751919.

17. Weber K. The use of chemiluminescence of luminol in forensic medicine and toxicology. I. Identification of blood stains. Dtsch Z Gesamte Gerichtl Med. 1966;57(3):410-23. PMid:5994184.

18. Bortoluzzi MC, Cadore P, GallonA, Imanishi SA. Forensic luminol blood test for preventing cross-contamination in dentistry: an evaluation of a dental school clinic. Int J Prev Med. 2014 Oct;5(10):1343-6. PMid:25400895.

19. Hamory BH, Whitener CJ. Nosocomial infections in dental, oral, and maxillofacial surgery. In: Mayhall CG. Hospital epidemiology and infection control. 2nd ed. Philadelphia: Lippincott Williams & Wilkins; 1999. p. 719-28.

20. Cleveland JL, Barker LK, Cuny EJ, Panlilio AL. Preventing percutaneous injuries among dental health care personnel. J Am Dent Assoc. 2007 Feb;138(2):169-78. PMid:17272371. http://dx.doi.org/10.14219/jada.archive.2007.0133.

21. Zarra T, Lambrianidis T. Percutaneous injuries amongst Greek endodontists: a national questionnaire survey.Int Endod J. 2013 Mar;46(3):264-74. PMid:23013210. http://dx.doi.org/10.1111/j.1365-2591.2012.02126.x.

22. Banker DD. Viral hepatitis (Part-II). Indian J Med Sci. 2003 Sep;57(9):415-24. PMid:14515033.

23. Ferreira LG. Synthesis of Luminol in a semi-pilot scale and development of new analytical applications [thesis]. Rio de Janeiro: Chemistry Institute, Federal University of Rio de Janeiro; 2009. In portuguese.

24. Frégeau CJ, Germain O, Fourney RM. Fingerprint enhancement revisited and the effects of blood enhancement chemicals on subsequent profiler plus fluorescent short tandem repeat DNA analysis of fresh and aged bloody fingerprints. J Forensic Sci. 2000 Mar;45(2):354-80. PMid:10782955. http://dx.doi.org/10.1520/JFS14688J.

25. Seashols SJ, Cross HD, Shrader DL, Rief A. A comparison of chemical enhancements for the detection of latent blood. J Forensic Sci. 2013 Jan;58(1):130-3. PMid:23033883. http://dx.doi.org/10.1111/j.1556-4029.2012.02259.x.

26. CreamerJI, Quickenden TI, Crichton LB, Robertson P, Ruhayel RA. Attempted cleaning of bloodstains and its effect on the forensic luminol test. Luminescence. 2005 Nov-Dec;20(6):411-3. PMid:15966054. http://dx.doi.org/10.1002/bio.865.

27. Stoica BA, Bunescu S, Neamtu A, Bulgaru-Iliescu D, Foia L, Botnariu EG. Improving luminol blood detection in forensics. J Forensic Sci. 2016 Sep;61(5):1331-6. http://dx.doi.org/10.1111/1556-4029.13141. PMid:27329571.

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