Revista de Odontologia da UNESP
ISSN 1807-2577 (Eletrônico)
Artigo Original

Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Atividade antibacteriana de medicações intracanal à base de hidróxido de cálcio e óxido de zinco micro- e nanoparticulado: estudo ex vivo

Juliane Maria GUERREIRO-TANOMARU; Alana Priscila SOUZA AGUIAR; Gisselle Moraima CHÁVEZ-ANDRADE; Maria Inês Basso BERNARDI; Mário TANOMARU-FILHO

Abstract

Abstract: Introduction: Nanoparticles and associations to calcium hydroxide can be used to increase antimicrobial activity.

Objective: To evaluate antibacterial activity against Enterococcus faecalis and pH of intracanal medications (IM) based on calcium hydroxide (CH) and zinc oxide (ZnO) micro- or nanoparticles, and their association with 0.4% chlorhexidine (CHX).

Material and method: Root canals from single-rooted human teeth were inoculated and incubated for 21 days. After sample (S1), the root canals were filled with the medications for 7 days and samples were collected immediately after medication (S2) and 7 days later (S3). Counting of CFU mL-1 was performed. Polyethylene tubes filled with the medications were used for the pH evaluation after 3, 7, 14 and 28 days. Data were submitted to ANOVA and Tukey tests (p<0.05).

Result: All IM promoted elimination of E. faecalis immediately after medication (S2). All the pastes promoted a similar pH increase.

Conclusion: CH/ZnO micro- or nanoparticles associated with CHX promoted greater bacterial reduction in the root canals and similar pH.

 

Keywords

Anti-infective agents, calcium hydroxide, endodontics, Enterococcus faecalis, nanoparticles

Resumo

Resumo: Introdução: Nanopartículas e associações ao hidróxido de cálcio podem ser usados para aumentar a ação antimicrobiana.

Objetivo: Avaliar a atividade antibacteriana sobre Enterococcus faecalis e o pH de medicações intracanal (MI) à base de hidróxido de cálcio (HC) e óxido de zinco (OZn) micro- e nanoparticulado, e suas associações com clorexidina (CHX) a 0,4%.

Material e método: Canais radiculares de dentes humanos unirradiculados foram inoculados e incubados por 21 dias. Após coleta (C1), os canais radiculares foram preenchidos com os medicamentos durante 7 dias e as amostras foram coletadas imediatamente após a medicação (C2) e 7 dias depois (C3). Contagem de UFC mL-1 foi realizada. Os tubos de polietileno preenchidos com os medicamentos foram utilizados para a avaliação do pH após 3, 7, 14 e 28 dias. Os dados foram submetidos aos testes de ANOVA e Tukey (p<0,05).

Resultado: Todas as MI promoveram eliminação de E. faecalis imediatamente após a medicação (C2). Todas as pastas promoveram similar aumento de pH.

Conclusão: HC/OZn micro- ou nanoparticulado associado com CHX promove maior redução bacteriana nos canais radiculares e pH similar.

Palavras-chave

Anti-infecciosos, hidróxido de cálcio, endodontia, Enterococcus faecalis, nanopartículas

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