Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.09417
Revista de Odontologia da UNESP
Original Article

In vitro evaluation of the antibacterial behavior of a self-etch adhesive associated with chlorhexidine

Avaliação in vitro da ação anti-bacteriana de um adesivo auto-condicionante acrescido de clorexidina

Roberta Bento RODRIGUES; Nereida Mello GIOPPO; Priscilla do Monte Ribeiro BUSATO; Marcio José MENDONÇA; Veridiana CAMILOTTI

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Abstract

Abstract: Introduction: Adhesive technology has been developed quickly since its introduction about fifty years ago. Its goal is to produce a close contact within enamel and dentin. Failures in this process could result in microleakage, and allow the infiltration of bacteria, fluids and ions. Recent studies have been shown the use of chlorhexidine associated with adhesive systems can be able to inhibit the bacteria action.

Objective: The aim of this study was to evaluate the addiction of different percentages of chlorhexidine to a self-etch adhesive.

Methodology: The solution of digluconatechlorhexidine was increased to the primer of the two pass self etch adhesive to create a 0.5%, 1.0% and 2.0% chlorhexidine primer solution and after they were distributed in four groups (G1, G2, G3 and G4). Saliva samples (N=10) were used to test bacteria activity.

Result: They were spread in a blood medium with filter paper disks containing the different treatments. After the incubation, the inhibitions halos formation were evaluated.

Conclusion: This study demonstrated that, in vitro, the addition of different percentages of chlorhexidine digluconate to the self-etch adhesive induced inhibited halos at bacteria of saliva samples, independent from their concentration.

Keywords

Dentin adhesive, dentin, anti-bacterial agents

Resumo

Resumo: Introdução: A tecnologia adesiva vem se desenvolvendo rapidamente desde que foi introduzida há mais de 50 anos. O principal objetivo da adesão é alcançar um íntimo contato entre a estrutura dental e o material restaurador e fornecer adequada união entre o esmalte e a dentina.

Objetivo: A proposta deste estudo foi avaliar o efeito antibacteriano de um adesivo auto-condicionante de dois passos associado a diferentes concentrações de solução de digluconato de clorexidina (DCHX).

Metodologia: A solução de digluconato de clorexidina a 20% foi adicionada ao primer do sistema adesivo nas concentrações de 0,5, 1,0 e 2,0% e distribuídas em quatro grupos (G1, G2, G3 e G4). Para o teste de atividade antibacteriana foram utilizadas amostras de saliva (N=10) semeadas em meio de ágar sangue e discos de papel filtro contendo os diferentes grupos. Após incubação, as amostras foram inspecionadas quanto à formação de halo de inibição dos microrganismos através da utilização do paquímetro digital de precisão (0,0001mm) (Mitutoyou Sul Americana Ltda/ Starret Tools ®).

Resultado: Halos de inibição foram encontrados ao redor de todos os discos de filtro em que foram aplicados o primer acrescido de clorexidina mais o adesivo, bem como ao redor dos discos do controle positivo. Ao redor dos discos do controle negativo e dos discos com primer e adesivo não modificado não foram observados halos de inibição.

Conclusão: Este trabalho demonstrou que, in vitro, a adição de diferentes concentrações de digluconato de clorexidina ao sistema adesivo produziu halo de inibição dos microrganismos presentes nas amostras de saliva, independente da concentração utilizada.
 

Palavras-chave

Adesivo dentinário, dentina, agente antibacteriano

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