Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
Avaliação da água de coco neutralizada por diferentes agentes na viabilidade de fibroblastos humanos: estudo in vitro
Soares, Priscilla Barbosa Ferreira; Rocha, Aletheia Moraes; Reis, Manuella Verdinelli de Paula; Moura, Camilla Christian Gomes; Soares, Carlos José
Abstract
Objective: This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method: Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result: There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion: The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.
Keywords
Resumo
Objetivo: Avaliar a eficácia de quatro tipos de substâncias usadas para ajuste do pH da água de coco (AC) sobre a viabilidade de fibroblastos humanos (HFF). Material e método: O pH da AC natural e industrializada foi ajustado para pH 7,0 utilizando: (1) Hidróxido de Sódio (NaOH), (2) bicarbonato de sódio (NaHCO3), (3) Trietanolamina (C6H15NO3), (4) 2-Amino-2- Methil-1-propanol (C4H11NO). Células HFF foram plaqueadas em 2×104 células/poço em placas de 96 poços e mantidas nas diferentes soluções de AC acima durante 2 h e 4 h. Controle positivo foi representado por HFF mantidas em DMEM e o controle negativo por água da torneira. A viabilidade celular foi avaliada pelo método de MTT Formazan. Os dados foram analisados por 3-way ANOVA seguido pelo teste de Tukey e Dunnett. Resultado: A viabilidade celular não é influenciada pelo período de avaliação, e as interações entre AC e período de avaliação, AC e método de ajuste de pH, método de ajuste de pH e período de avaliação (p>0,05). Conclusão: O produto utilizado para ajuste do pH não interfere na viabilidade de FH, embora, haja uma tendência de melhor desempenho em AC natural.
Palavras-chave
References
1. Rajendran P, Varghese NO, Varughese JM, Murugaian E. Evaluation, using extracted human teeth, of Ricetral as a storage medium for avulsions: an in vitro study. Dent Traumatol. 2011 Jun;27(3):217-20. http://dx.doi.org/10.1111/j.1600-9657.2011.00988.x. PMid:21535405.
2. Andreasen JO. Effect of extra-alveolar period and storage media upon periodontal and pulpal healing after replantation of mature permanent incisors in monkeys. Int J Oral Surg. 1981 Feb;10(1):43-53. http://dx.doi.org/10.1016/S0300-9785(81)80007-5. PMid:6792094.
3. Trope M. Avulsion of permanent teeth: theory to practice. Dent Traumatol. 2011 Aug;27(4):281-94. http://dx.doi.org/10.1111/j.1600-9657.2011.01003.x. PMid:21635689.
4. Andreasen JO, Andreasen FM, Skeie A, Hjørting-Hansen E, Schwartz O. Effect of treatment delay upon pulp and periodontal healing of traumatic dental injuries: a review article. Dent Traumatol. 2002 Jun;18(3):116-28. http://dx.doi.org/10.1034/j.1600-9657.2002.00079.x. PMid:12110104.
5. Souza BD, Lückemeyer DD, Felippe WT, Simões CM, Felippe MC. Effect of temperature and storage media on human periodontal ligament fibroblast viability. Dent Traumatol. 2010 Jun;26(3):271-5. http://dx.doi.org/10.1111/j.1600-9657.2010.00886.x. PMid:20572843.
6. Lindskog S, Blomlöf L, Hammarström L. Mitoses and microorganisms in the periodontal membrane after storage in milk or saliva. Scand J Dent Res. 1983 Dec;91(6):465-72. PMid:6581523.
7. Ozan F, Polat ZA, Er K, Ozan U, Değer O. Effect of propolis on survival of periodontal ligament cells: new storage media for avulsed teeth. J Endod. 2007 May;33(5):570-3. http://dx.doi.org/10.1016/j.joen.2006.12.021. PMid:17437874.
8. Goswami M, Chaitra TR, Chaudhary S, Manuja N, Sinha A. Strategies for periodontal ligament cell viability: An overview. J Conserv Dent. 2011 Jul-Sep;14(3):215-20. http://dx.doi.org/10.4103/0972-0707.85789. PMid:22025820.
9. Udoye CI, Jafarzadeh H, Abbott PV. Transport media for avulsed teeth: a review. Aust Endod J. 2012 Dec;38(3):129-36. http://dx.doi.org/10.1111/j.1747-4477.2012.00356.x. PMid:23211073.
10. Moura CC, Soares PB, Reis MV, Fernandes AJ No, Soares CJ. Soy milk as a storage medium to preserve human fibroblast cell viability: an in vitro study. Braz Dent J. 2012;23(5):559-63. http://dx.doi.org/10.1590/S0103-64402012000500015. PMid:23306234.
11. Moura CC, Soares PB, Reis MVP, Fernandes Neto AJ, Barbosa DZ, Soares CJ. Potential of coconut water and soy milk for use as storage media to preserve the viability of periodontal ligament cells: an in vitro study. Dent Traumatol. 2014 Feb;30(1):22-6. http://dx.doi.org/10.1111/edt.12042. PMid:23566116.
12. Gopikrishna V, Thomas T, Kandaswamy D. A quantitative analysis of coconut water: a new storage media for avulsed teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Feb;105(2):e61-5. http://dx.doi.org/10.1016/j.tripleo.2007.08.003. PMid:18230380.
13. Gopikrishna V, Baweja PS, Venkateshbabu N, Thomas T, Kandaswamy D. Comparison of coconut water, propolis, HBSS, and milk on PDL cell survival. J Endod. 2008 May;34(5):587-9. http://dx.doi.org/10.1016/j.joen.2008.01.018. PMid:18436040.
14. Moreira-Neto JJ, Gondim JO, Raddi MS, Pansani CA. Viability of human fibroblasts in coconut water as a storage medium. Int Endod J. 2009 Sep;42(9):827-30. http://dx.doi.org/10.1111/j.1365-2591.2009.01591.x. PMid:19549148.
15. Reis MVP, Moura CC, Soares PB, Leoni GB, Souza-Neto MD, Barbosa DZ, et al. Histologic and micro-computed tomographic analyses of replanted teeth stored in different kind of media. J Endod. 2014 May;40(5):665-9. http://dx.doi.org/10.1016/j.joen.2013.09.023. PMid:24767561.
16. Campbell-Falck D, Thomas T, Falck TM, Tutuo N, Clem K. The intravenous use of coconut water. Am J Emerg Med. 2000 Jan;18(1):108-11. http://dx.doi.org/10.1016/S0735-6757(00)90062-7. PMid:10674546.
17. Thomas T, Gopikrishna V, Kandaswamy D. Comparative evaluation of maintenance of cell viability of an experimental transport media “coconut water” with Hank’s balanced salt solution and milk, for transportation of an avulsed tooth: An in vitro cell culture study. J Conserv Dent. 2008 Jan-Mar;11(1):22-9. http://dx.doi.org/10.4103/0972-0707.43414. PMid:20142880.
18. Sanghavi T, Shah N, Parekh V, Singbal K. Evaluation and comparison of efficacy of three different storage media, coconut water, propolis, and oral rehydration solution, in maintaining the viability of periodontal ligament cells. J Conserv Dent. 2013 Jan;16(1):71-4. http://dx.doi.org/10.4103/0972-0707.105303. PMid:23349581.
19. Souza BD, Lückemeyer DD, Reyes-Carmona JF, Felippe WT, Simões CM, Felippe MC. Viability of human periodontal ligament fibroblasts in milk, Hank’s balanced salt solution and coconut water as storage media. Int Endod J. 2011 Feb;44(2):111-5. http://dx.doi.org/10.1111/j.1365-2591.2010.01809.x. PMid:21083571.
20. Prades A, Dornier M, Diop N, Pain J-P. Coconut water preservation and processing: a review. Fruits. 2012 May-Jun;67(3):157-71. http://dx.doi.org/10.1051/fruits/2012009.
21. Carvalho JMC, Maia GA, Souza PHM, Maia GA Jr. Água de coco: propriedades nutricionais, funcionais e processamento. Ciências Agropecuárias. 2006 Jul-Set;27(3):437-52.
22. Blomlöf L. Milk and saliva as possible storage media for traumatically exarticulated teeth prior to replantation. Swed Dent J Suppl. 1981;8:1-26. PMid:6942523.
23. Blomlöf L. Storage of human periodontal ligament cells in a combination of different media. J Dent Res. 1981 Nov;60(11):1904-6. http://dx.doi.org/10.1177/00220345810600111301. PMid:6945330.
24. Silva EJ, Rollemberg CB, Coutinho-Filho TS, Zaia AA. A multiparametric assay to compare the cytotoxicity of soy milk with different storage media. Dent Traumatol. 2013 Aug;29(4):319-22. http://dx.doi.org/10.1111/j.1600-9657.2012.01175.x. PMid:22882901.
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email