Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.06719
Revista de Odontologia da UNESP
Original Article

Análise imuno-histoquímica de Ki-67 e α-SMA em ceratocisto odontogênico, ameloblastoma e folículo pericoronário

Immunohistochemical analysis of Ki-67 and α-SMA in odontogenic keratocyst, ameloblastoma and pericoronal follicle

Lígia Figueiredo VALESAN; Andressa Fernanda Paza MIGUEL; Grasieli de Oliveira RAMOS; Elena Riet Correa RIVERO; Kamile Leonardi DUTRA-HORSTMANN

http://dx.doi.org/10.1590/1807-2577.06719 Rev. odontol. UNESP, vol.48, e20190067, 2019
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Resumo

Resumo: Introdução: Os ameloblastomas (AM) são considerados os tumores odontogênicos mais comuns da cavidade bucal, apresentando grande importância clínica devido à sua agressividade, capacidade infiltrativa e comportamento recorrente. De maneira semelhante, o ceratocisto odontogênico (CO) desperta a atenção por ter um comportamento agressivo e altas taxas de recorrência em relação aos outros cistos de desenvolvimento.

Objetivo: Avaliar e comparar o índice de proliferação epitelial e a presença de miofibroblastos em CO e AM, por meio dos anticorpos Ki-67 e α-SMA, respectivamente.

Metodologia: Foram selecionados 15 casos de AM e 24 casos de CO para investigação imuno-histoquímica das proteínas Ki-67 e α-SMA. Um grupo de sete folículos pericoronários (FP) foi incluído como controle de tecido odontogênico normal. A média de células positivas foi calculada para cada marcador.

Resultado: O teste de Kruskal-Wallis revelou que a expressão de ambos os marcadores foi maior nos casos de CO, quando comparada à expressão em AM e FP. Segundo o teste de Mann-Whitney, a expressão dos marcadores foi semelhante entre os subtipos de AM.

Conclusão: A alta expressão de Ki-67 e α-SMA observada em CO poderia estar associada ao comportamento agressivo desta lesão em relação aos outros cistos de desenvolvimento. Por outro lado, a expressão semelhante destas proteínas nos casos de AM e FP, assim como nos subtipos de AM, poderia indicar que outros fatores, além do potencial proliferativo, estariam associados ao comportamento clínico agressivo do AM.

Palavras-chave

Ameloblastoma, cistos odontogênicos, antígeno Ki-67, miofibroblastos

Abstract

Abstract: Introduction: Ameloblastomas (AM) are considered the most common odontogenic tumors, with great clinical importance linked to its locally aggressive, infiltrative, and recurrent behavior. The odontogenic keratocyst (OK) represents a peculiar cyst that also draws attention because of its aggressive behavior and high rates of local recurrence.

Objective: The aim of this study was to evaluate the cell proliferation index and the presence of myofibroblasts in AM and OK using Ki-67 and α-SMA antibodies, respectively.

Methodology: Fifteen cases of AM and 24 cases of OK were selected for immunohistochemical investigation of the proteins α-SMA and Ki-67. A group of 7 pericoronal follicles (PF) was included as control for normal odontogenic tissue. The mean percentage of positive cells was calculated for each protein.

Result: Kruskal-Wallis test for both markers showed that expression was higher in OK, when compared to AM and PF. Mann-Whitney test showed no statistical difference when comparing the expression of both markers between the subtypes of AM.

Conclusion: Elevated expression of Ki-67 and α-SMA in OK may be associated with the aggressive clinical behavior of this cyst in comparison to other development cysts. The similar expression of the markers observed among AM and PF cases, as well as among subtypes of AM, may indicate that other factors, besides proliferative potential, are associated with the aggressive behavior of AM.
 

Keywords

Ameloblastoma, odontogenic cysts, antigen Ki-67, myofibroblast

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