Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.05915
Revista de Odontologia da UNESP
Original Article

Assessment of the effects of decontamination and storage methods on the structural integrity of human enamel

Avaliação dos efeitos de métodos de descontaminação e armazenamento sobre a integridade estrutural do esmalte humano

Freitas, Adriana Rodrigues de; Aznar, Fábio Duarte da Costa; Silva, André Luís da; Sales-Peres, Arsenio; Sales-Peres, Silvia Helena de Carvalho

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Abstract

Introduction: The storage of teeth for use in research is a controversial issue with no consensus on the most appropriate treatment method for this purpose. Objective: The aim of this study was analyze the effectiveness and the influence of different methods of decontamination and storage of human enamel samples, in order to maintain their integrity. Material and method: The sample consisted of 124 molars distributed randomly into three groups according to the method: control - distilled water, 0.1% thymol and 0.02% sodium azide. The tests performed were laser fluorescence, surface microhardness and profilometry analysis (0, 15 and 30 days) and Microbiological test (7, 15 and 30 days). Data were analyzed by the ANOVA and Tukey tests (P <0.05). Result: In the laser fluorescence test, thymol proved to be more feasible for maintaining the integrity of the dental organ, since it did not show significant variations in values among the analyzed periods (P> 0.05). The surface microhardness analysis showed loss of tooth structure in all methods, and sodium azide led to a lower level of tooth loss. Profilometry analysis showed loss of mass in all groups whereas sodium azide showed the greatest loss. None of the methods was able to inhibit bacterial growth. Conclusion: Among the processing methods analyzed none was able to combine effective decontamination and storage with maintenance of the structural integrity of the human enamel.

Keywords

Dental enamel, infection, hardness, fluorescence.

Resumo

Introdução: O armazenamento de dentes para utilização em pesquisas é uma questão controvérsia e não há consenso sobre o método de tratamento mais apropriado para esta finalidade. Objetivo: O objetivo deste estudo foi analisar a efetividade e a influência de diferentes métodos de descontaminação e armazenamento do esmalte dentário humano, a fim de manter sua integridade. Material e método: A amostra foi constituída por 124 molares distribuídos aleatoriamente em três grupos, de acordo com o método: controle – água destilada, soluções de timol a 0,1% e de azida de sódio a 0,02%. Os testes realizados foram fluorescência a laser, microdureza de superfície e análise de perfilometria (0, 15 e 30 dias) e teste microbiológico (7, 15 e 30 dias). Os dados foram analisados por meio dos testes ANOVA e Tukey (p<0,05). Resultado: No teste de fluorescência a laser, a solução de timol a 0,1% demonstrou ser mais viável na manutenção da integridade do órgão dentário, uma vez que este não apresentou variações significativas nos valores entre os períodos avaliados (p<0,05). A análise de microdureza de superfície evidenciou perda de estrutura dentária em todos os métodos, sendo que a azida de sódio promoveu menor perda dentária. Na análise de perfilometria observou-se perda de estrutura em todos os grupos, com maior perda no grupo azida de sódio. Nenhum dos métodos conseguiu inibir o crescimento bacteriano. Conclusão: Dentre os métodos de processamento analisados nenhum foi capaz de aliar a efetividade na descontaminação ao armazenamento com manutenção da integridade estrutural do esmalte dentário humano.

Palavras-chave

Esmalte dentário, infecção, dureza, fluorescência.

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