Revista de Odontologia da UNESP
https://revodontolunesp.com.br/article/doi/10.1590/1807-2577.03423
Revista de Odontologia da UNESP
Original Article

A new proposal for evaluating of the solubility of bioceramic materials in dentin tubes after immersion in PBS: a laboratory investigation

Nova proposta para avaliação da solubilidade de materiais biocerâmicos em tubos de dentina após imersão em PBS: um estudo laboratorial

Giovanna da Cunha MENDONÇA; Karina Ines Medina Carita TAVARES; Airton Oliveira SANTOS-JUNIOR; Jáder Camilo PINTO; Juliane Maria GUERREIRO-TANOMARU; Mário TANOMARU-FILHO

http://dx.doi.org/10.1590/1807-2577.03423 Rev. odontol. UNESP, vol.52, e20230034, 2023
PDF
Downloads: 0
Views: 368

Abstract

Introduction: Repair materials must have low solubility. Phosphate buffered saline (PBS) allows simulating clinical condition and interaction with dentin may be important for the correct evaluate of mass loss of bioceramic cements.

Objective: To evaluate the effect of distilled water (DW), or PBS immersion on the solubility of Bio-C Repair (BCR, Angelus) or MTA Repair HP (MTAHP, Angelus) using a dentin tube model.

Material and method: Bovine dentin tubes with a length of 4 mm, an internal diameter of 1.5 mm and walls thickness of approximately 1 mm were made. The specimens were immersed in DW for 24h, then filled with BCR or MTAHP (n = 14) and stored in an oven at 37°C and 95% humidity for 24h. After being weighed on a precision balance to determine the initial mass, the specimens were immersed in DW (pH 6.5) or PBS (pH 7.0) (n = 7) for 28 days. Empty tubes also were used for calculating the mass loss of the dentin (n=4). After this period, the specimens were weighed until stabilization of the final mass occurred (0.001g). The solubility of each material was evaluated. ANOVA and Tukey statistical tests were performed (α=0.05).

Result: BCR and MTAHP showing gain of mass in DW and mass loss in PBS (p<0.05).

Conclusion: The immersion solution influenced the solubility of BCR and MTAHP using dentin tube model. The new methodological proposal could be an alternative to ISO standards for testing the solubility of bioceramic cements.

Keywords

Calcarea silicata, endodontics, dental materials, physical properties

Resumo

Introdução: Materiais reparadores devem apresentar baixa solubilidade. Solução salina tamponada com fosfato (PBS) permite simular condição clínica e interação com dentina pode ser importante para correta avaliação da perda de massa de cimentos biocerâmicos.

Objetivo: Avaliou o efeito da imersão em água destilada (AD) ou PBS na solubilidade de Bio-C Repair (BCR, Angelus) ou MTA Repair HP (MTAHP, Angelus) usando modelo de tubo de dentina.

Material e método: Tubos de dentina bovina foram confeccionados com 4 mm de comprimento, 1,5 mm de diâmetro interno e 1 mm aproximadamente de espessura de parede. Os espécimes foram imersos em AD por 24h, posteriormente preenchidos com BCR ou MTAHP (n = 14) e armazenados em estufa a 37°C e umidade 95% por 24h. Após serem pesados em balança de precisão para determinação da massa inicial, os corpos de prova foram imersos em AD (pH 6,5) ou PBS (pH 7,0) (n = 7) por 28 dias. Tubos vazios também foram utilizados para o cálculo de perda de massa da dentina (n=4). Após esse período, os espécimes foram pesados até a estabilização da massa final (0,001g). A solubilidade de cada material foi avaliada. Testes estatísticos ANOVA e Tukey foram realizados (α=0,05).

Resultado: BCR e MTAHP apresentaram ganho de massa em AD e perda de massa em PBS (p<0,05).

Conclusão: A solução de imersão influencia a solubilidade de BCR e MTAHP usando modelo de tubo de dentina. Nova proposta metodológica poderá ser uma alternativa às normas ISO para testar a solubilidade de cimentos biocerâmicos.

Palavras-chave

Calcarea silicata, endodontia, materiais dentários, propriedades físicas

References

1 Toubes KS, Tonelli SQ, Girelli CFM, Azevedo CGS, Thompson ACT, Nunes E, et al. Bio-C repair - a new bioceramic material for root perforation management: two case reports. Braz Dent J. 2021;32(1):104-10. http://dx.doi.org/10.1590/0103-6440202103568. PMid:33913996.

2 Cavenago BC, Pereira TC, Duarte MAH, Ordinola-Zapata R, Marciano MA, Bramante CM, et al. Influence of powder-to-water ratio on radiopacity, setting time, pH, calcium ion release and a micro-CT volumetric solubility of white mineral trioxide aggregate. Int Endod J. 2014;47(2):120-6. http://dx.doi.org/10.1111/iej.12120. PMid:23647286.

3 Sanz JL, Forner L, Llena C, Guerrero-Gironés J, Melo M, Rengo S, et al. Cytocompatibility and bioactive properties of hydraulic calcium silicate-based cements (HCSCs) on stem cells from human exfoliated deciduous teeth (SHEDs): a systematic review of in vitro studies. J Clin Med. 2020;9(12):3872. http://dx.doi.org/10.3390/jcm9123872. PMid:33260782.

4 Palczewska-Komsa M, Kaczor-Wiankowska K, Nowicka A. New bioactive calcium silicate cement mineral trioxide aggregate repair high plasticity (MTA HP)-a systematic review. Materials (Basel). 2021;14(16):4573. http://dx.doi.org/10.3390/ma14164573. PMid:34443098.

5 Guimarães BM, Prati C, Duarte MAH, Bramante CM, Gandolfi MG. Physicochemical properties of calcium silicate-based formulations MTA repair HP and MTA Vitalcem. J Appl Oral Sci. 2018;26(0):e2017115. http://dx.doi.org/10.1590/1678-7757-2017-0115. PMid:29641748.

6 Quintana RM, Jardine AP, Grechi TR, Grazziotin-Soares R, Ardenghi DM, Scarparo RK, et al. Bone tissue reaction, setting time, solubility, and pH of root repair materials. Clin Oral Investig. 2019;23(3):1359-66. http://dx.doi.org/10.1007/s00784-018-2564-1. PMid:30022271.

7 International Organization for Standardization. ISO 6876: dental root canal sealing materials. Geneva: International Organization for Standardization; 2012.

8 American National Standards Institute – ANSI; American Dental Association – ADA. Specification no. 57 ADA. Laboratory testing methods: endodontic filling and sealing materials: Endodontic Sealing Materials. Chicago, USA: ANSI, ADA; 2000.

9 Gandolfi MG, Siboni F, Botero T, Bossù M, Riccitiello F, Prati C. Calcium silicate and calcium hydroxide materials for pulp capping: biointeractivity, porosity, solubility and bioactivity of current formulations. J Appl Biomater Funct Mater. 2015;13(1):43-60. http://dx.doi.org/10.5301/jabfm.5000201. PMid:25199071.

10 Elyassi Y, Moinzadeh AT, Kleverlaan CJ. Characterization of leachates from 6 root canal sealers. J Endod. 2019;45(5):623-7. http://dx.doi.org/10.1016/j.joen.2019.01.011. PMid:30905572.

11 Urban K, Neuhaus J, Donnermeyer D, Schäfer E, Dammaschke T. Solubility and pH value of 3 different root canal sealers: a long-term investigation. J Endod. 2018;44(11):1736-40. http://dx.doi.org/10.1016/j.joen.2018.07.026. PMid:30243663.

12 Reyes-Carmona JF, Felippe MS, Felippe WT. A phosphate-buffered saline intracanal dressing improves the biomineralization ability of mineral trioxide aggregate apical plugs. J Endod. 2010;36(10):1648-52. http://dx.doi.org/10.1016/j.joen.2010.06.014. PMid:20850670.

13 Benetti F, Queiroz IOA, Cosme-Silva L, Conti LC, Oliveira SHP, Cintra LTA. Cytotoxicity, biocompatibility and biomineralization of a new ready-for-use bioceramic repair material. Braz Dent J. 2019;30(4):325-32. http://dx.doi.org/10.1590/0103-6440201902457. PMid:31340221.

14 Oliveira LV, Souza GL, Silva GR, Magalhães TEA, Freitas GAN, Turrioni AP, et al. Biological parameters, discolouration and radiopacity of calcium silicate-based materials in a simulated model of partial pulpotomy. Int Endod J. 2021;54(11):2133-44. http://dx.doi.org/10.1111/iej.13616. PMid:34418112.

15 Torres FF, Pinto JC, Figueira GO, Guerreiro-Tanomaru JM, Tanomaru-Filho M. A micro-computed tomographic study using a novel test model to assess the filling ability and volumetric changes of bioceramic root repair materials. Restor Dent Endod. 2020;46(1):e2. http://dx.doi.org/10.5395/rde.2021.46.e2. PMid:33680891.

16 Inada RNH, Queiroz MB, Lopes CS, Silva ECA, Torres FFE, Silva GF, et al. Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model. Clin Oral Investig. 2023;27(7):3839-53. http://dx.doi.org/10.1007/s00784-023-05002-5. PMid:37014506.

17 Vergaças JHN, de Lima CO, Barbosa AFA, Vieira VTL, Santos Antunes H, Silva EJNL. Marginal gaps and voids of three root-end filling materials: a microcomputed tomographic study. Microsc Res Tech. 2022;85(2):617-22. http://dx.doi.org/10.1002/jemt.23935. PMid:34516035.

18 Ferreira CMA, Sassone LM, Gonçalves AS, de Carvalho JJ, Tomás-Catalá CJ, García-Bernal D, et al. Physicochemical, cytotoxicity and in vivo biocompatibility of a high-plasticity calcium-silicate based material. Sci Rep. 2019;9(1):3933. http://dx.doi.org/10.1038/s41598-019-40365-4. PMid:30850648.

19 Acris De Carvalho FM, Silva-Sousa YTC, Saraiva Miranda CE, Miller Calderon PH, Barbosa AFS, Domingues De Macedo LM, et al. Influence of ultrasonic activation on the physicochemical properties of calcium silicate-based cements. Int J Dent. 2021;2021:6697988. http://dx.doi.org/10.1155/2021/6697988. PMid:33574844.

20 Delfino MM, Abreu Jampani JL, Lopes CS, Guerreiro-Tanomaru JM, Tanomaru-Filho M, Sasso-Cerri E, et al. Comparison of Bio-C Pulpo and MTA Repair HP with White MTA: effect on liver parameters and evaluation of biocompatibility and bioactivity in rats. Int Endod J. 2021;54(9):1597-613. http://dx.doi.org/10.1111/iej.13567. PMid:33999424.

21 Galarça AD, Rosa WLO, Silva TM, Silveira Lima G, Carreño NLV, Pereira TM, et al. Physical and biological properties of a high-plasticity tricalcium silicate cement. BioMed Res Int. 2018;2018:8063262. http://dx.doi.org/10.1155/2018/8063262. PMid:30622963.

22 Pelepenko LE, Saavedra F, Antunes TBM, Bombarda GF, Gomes BPFA, Zaia AA, et al. Investigation of a modified hydraulic calcium silicate-based material - Bio-C Pulpo. Braz Oral Res. 2021;35:e077. http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0077. PMid:34161414.

23 ElReash AA, Hamama H, Eldars W, Lingwei G, Zaen El-Din AM, Xiaoli X. Antimicrobial activity and pH measurement of calcium silicate cements versus new bioactive resin composite restorative material. BMC Oral Health. 2019;19(1):235. http://dx.doi.org/10.1186/s12903-019-0933-z. PMid:31684929.

24 Queiroz MB, Torres FFE, Rodrigues EM, Viola KS, Bosso-Martelo R, Chavez-Andrade GM, et al. Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers. Dent Mater. 2021;37(2):311-20. http://dx.doi.org/10.1016/j.dental.2020.11.014. PMid:33323301.

25 Ochoa-Rodríguez VM, Tanomaru-Filho M, Rodrigues EM, Guerreiro-Tanomaru JM, Spin-Neto R, Faria G. Addition of zirconium oxide to Biodentine increases radiopacity and does not alter its physicochemical and biological properties. J Appl Oral Sci. 2019;27:e20180429. http://dx.doi.org/10.1590/1678-7757-2018-0429. PMid:30970115.

26 Siboni F, Taddei P, Zamparini F, Prati C, Gandolfi MG. Properties of BioRoot RCS, a tricalcium silicate endodontic sealer modified with povidone and polycarboxylate. Int Endod J. 2017;50(S2 Suppl 2):e120-36. http://dx.doi.org/10.1111/iej.12856. PMid:28881478.

27 Zordan-Bronzel CL, Tanomaru-Filho M, Chávez-Andrade GM, Torres FFE, Abi-Rached GPC, Guerreiro-Tanomaru JM. Calcium silicate-based experimental sealers: physicochemical properties evaluation. Mater Res. 2021;24(1):e20200243. http://dx.doi.org/10.1590/1980-5373-mr-2020-0243.

28 Torres FFE, Bosso-Martelo R, Espir CG, Cirelli JA, Guerreiro-Tanomaru JM, Tanomaru Filho M. Methods using micro-CT for evaluating physicochemical properties and volumetric changes in root-end filling materials. J Appl Oral Sci. 2017;25(4):374-80. http://dx.doi.org/10.1590/1678-7757-2016-0454. PMid:28877275.

29 Bodanezi A, Carvalho N, Silva D, Bernardineli N, Bramante CM, Garcia RB, et al. Immediate and delayed solubility of mineral trioxide aggregate and Portland cement. J Appl Oral Sci. 2008;16(2):127-31. http://dx.doi.org/10.1590/S1678-77572008000200009. PMid:19089204.

30 Torres FFE, Guerreiro-Tanomaru JM, Bosso-Martelo R, Chavez-Andrade GM, Tanomaru-Filho M. Solubility, porosity and fluid uptake of calcium silicate-based cements. J Appl Oral Sci. 2018;26(0):e20170465. http://dx.doi.org/10.1590/1678-7757-2017-0465. PMid:29791569.

Submitted date:
11/04/2023

Accepted date:
11/05/2023

6569f895a9539504b121f684 rou Articles
Links & Downloads
1807-2577 (Electronic)
Rev. odontol. UNESP ©2024 All rights reserved.

Rev. odontol. UNESP

Share this page
Page Sections