Revista de Odontologia da UNESP
http://revodontolunesp.com.br/article/doi/10.1590/1807-2577.02318
Revista de Odontologia da UNESP
Original Article

Influence of a new method of sterilization on the morphology and physical properties of extracted human teeth

Influência de um novo método de esterilização na morfologia e propriedades físicas de dentes humanos extraídos

Daylana Pacheco da SILVA; Urias Silva VASCONCELOS; Valdimar da Silva VALENTE; Gregório Antônio Soares MARTINS; Carmem Dolores Vilarinho Soares de MOURA

Abstract

Abstract: Introduction: Sterilization methods adopted in Human Teeth Bank can promote structural changes in enamel and dentin. Thus, the ideal method should combine the antimicrobial efficacy and preservation of the substrate biomechanical properties.

Aim: Evaluated the morphology and physical properties of enamel and dentin of extracted human teeth, after being submitted to different sterilization methods.

Method: Sixteen extracted third molars were sectioned in the following tooth regions: mesio-distal, vestibular-lingual and at the cement enamel junction. Forty specimens were selected according to values of microhardness and roughness initials, and distributed in four experimental groups (n = 10/group): Group 1 = Autoclave 121 °C (30 minutes); Group 2 = 2.5% Sodium hypochlorite (07 days); Group 3 = 5.25% Sodium hypochlorite (07 days); Group 4= 30% Acetic Acid (7 days). After sterilization, the microhardness, roughness and morphology of enamel and dentin were evaluated. Data were analyzed by paired t-test, Wilcoxon, Kruskal-Wallis and Analysis of Variance. A significance level of 5% was adopted.

Result: All sterilization methods altered the microhardness and roughness of the dentine (p <0.05). Regarding to enamel, the microhardness was affected only by Autoclave and 2.5% Sodium hypochlorite treatment (p <0.05). A large aperture of the enamel pores and dentinal tubules was verified in the micrographs.

Conclusion: The sterilization methods adopted altered the morphology and/or physical properties of enamel and dentine.

Keywords

Dental enamel, hardness, sterilization, dentin

Resumo

Resumo: Introdução: Métodos de esterilização adotados em Banco de Dentes Humanos podem promover mudanças estruturais no esmalte e na dentina. Assim, o método ideal deve combinar a eficácia antimicrobiana e a preservação das propriedades biomecânicas do substrato.

Objetivo: Avaliar a morfologia e propriedades físicas do esmalte e dentina dos dentes humanos extraídos, após serem submetidos a diferentes métodos de esterilização.

Método: Dezesseis terceiros molares extraídos foram seccionados nas seguintes regiões dentárias: mesio-distal, vestibulo-lingual e na junção amelocementária. Quarenta espécimes foram selecionados de acordo com valores de microdureza e rugosidade iniciais, e distribuídos em quatro grupos experimentais (n = 10 / grupo): Grupo 1 = Autoclave 121 °C (30 minutos); Grupo 2 = 2,5% de hipoclorito de sódio (07 dias); Grupo 3 = 5,25% Hipoclorito de sódio (07 dias); Grupo 4 = ácido acético a 30% (7 dias). Após a esterilização, foi avaliada a microdureza, rugosidade e morfologia do esmalte e dentina. Os dados foram analisados ​​pelo teste t pareado, Wilcoxon, Kruskal-Wallis e Análise de Variância. Um nível de significância de 5% foi adotado.

Resultado: Todos os métodos de esterilização alteraram a microdureza e a rugosidade da dentina (p <0,05). Em relação ao esmalte, a microdureza foi afetada apenas pela Autoclave e pelo tratamento com 2,5% de hipoclorito de sódio (p <0,05). Foi verificada nas micrografias, uma grande abertura dos poros do esmalte e dos túbulos dentinários.

Conclusão: os métodos de esterilização adotados alteraram a morfologia e / ou propriedades físicas do esmalte e da dentina.
 

Palavras-chave

Esmalte dentário, dureza, esterilização, dentina

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