Revista de Odontologia da UNESP
Revista de Odontologia da UNESP
Original Article

In vitro effect of energy drinks on human enamel surface

Análise in vitro da ação de bebidas energéticas no esmalte dental humano

Marise Sano Suga MATUMOTO; Raquel Sano Suga TERADA; Dayla Thyeme HIGASHI; Mitsue FUJIMAKI; Selma Sano SUGA; Antônio Carlos GUEDES-PINTO

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Introduction: Energy drinks (ED) possess low pH and citric acid in their composition, making them potentially erosive beverages that can contribute to the high dental erosion rates found currently in the general population and also in young people.

Objective: To evaluate the mean pH and titratable acidity of commercial ED and the influence of a brand of ED on the superficial microhardness of human enamel.

Material and method: Ten commercial ED were selected and the pH of two lots of each ED with and without gas was obtained. Acid titration was conducted with the addition of NaOH aliquots until the pH 7 was reached. Eighteen human enamel specimens were allocated in three groups (N=6), Red Bull (RB), Red Bull Light (RBL) and distilled water (C), submitted to an acid challenge with the ED, six consecutive times, with 12 hours intervals, during three days. Knoop microhardness was measured before and after the acid challenge.

Result: All ED brands tested presented low pH levels ranging from 2.1 to 3.2. Regarding titratable acidity, it was found that the amount of base required promoting the neutralization of the solutions ranged from 1200μL to 3750μL. Samples of human enamel in the RB and RBL groups submitted to the acid challenge presented significantly decreased Knoop microhardness when compared with the group C.

Conclusion: All ED examined have potential to promote mineral loss due to the low pH and high titratable acidity. The ED analyzed promoted significant mineral losses on the dental enamel surface.


Tooth erosion, energy drinks, dental enamel, tooth demineralization


Introdução: O potencial erosivo das bebidas energéticas (BE) devido ao baixo pH e à presença de ácido cítrico pode estar relacionado ao aumento dos índices de erosão dental da população em geral e especialmente, nos jovens.

Objetivo: Verificar o pH e a titulação ácida de BE e a influência de uma marca de BE na microdureza superficial do esmalte.

Material e método: Dez amostras de BE de diferentes marcas comerciais foram selecionadas. O pH de dois lotes de cada BE foi analisado, com e sem gás. A titulação ácida foi realizada com a adição de alíquotas de NaOH, até atingir pH 7,0. Dezoito amostras de esmalte dental humano foram distribuídas aleatoriamente em três grupos (n = 6), Red Bull (RB), Red Light Bull (RBL) e água destilada (C), submetidas a um desafio ácido com a BE, seis vezes consecutivas, com intervalos de 12 horas, durante três dias. A microdureza Knoop foi medida antes e depois do desafio ácido.

Resultado: Todas as marcas de BE testadas apresentaram baixos níveis de pH, variando de 2,1 a 3,2. Em relação à titulação ácida, verificou-se que a quantidade de base necessária para promover a neutralização das soluções variou de 1200 μL a 3750 μL. Amostras de esmalte humano nos grupos RB e RBL submetidos ao desafio ácido apresentaram diminuição significativa da microdureza Knoop.

Conclusão: Todas as BE examinadas apresentaram baixo pH e alta titulação ácida e, portanto, potencial para promover perda mineral. As BE analisadas promoveram perdas minerais significativas na superfície do esmalte dental.


Erosão dentária, alimentos para praticantes de atividade física, esmalte dentário, desmineralização do dente


Lara B, Gonzalez-Millán C, Salinero JJ, Abian-Vicen J, Areces F, Barbero-Alvarez JC, et al. Caffeine-containing energy drink improves physical performance in female soccer players. Amino Acids. 2014 May;46(5):1385-92. PMid:24615239.

Deliens T, Clarys P, Bourdeaudhuij I, Deforche B. Correlates of university students’ soft and energy drink consumption according to gender and residency. Nutrients. 2015 Aug;7(8):6550-66. PMid:26258790.

Nowak D, Jasionowski A. Analysis of the consumption of caffeinated energy drinks among polish adolescents. Int J Environ Res Public Health. 2015 Jul;12(7):7910-21. PMid:26184263.

Kao RT, Harpenau LA. Dental erosion and tooth wear. J Calif Dent Assoc. 2011 Apr;39(4):222-4. PMid:21675674.

Tahmassebi JF, Duggal MS, Malik-Kotru G, Curzon ME. Soft drinks and dental health: a review of the current literature. J Dent. 2006 Jan;34(1):2-11 [Published Online Sep 12, 2005]. PMid:16157439.

Parry J, Shaw L, Arnaud MJ, Smith AJ. Investigation of mineral waters and soft drinks in relation to dental erosion. J Oral Rehabil. 2001 Aug;28(8):766-72. PMid:11556958.

Coombes JS. Sports drinks and dental erosion. Am J Dent. 2005 Apr;18(2):101-4. PMid:15973827.

Kitchens M, Owens BM. Effect of carbonated beverages, coffee, sports and high energy drinks, and bottled water on the in vitro erosion characteristics of dental enamel. J Clin Pediatr Dent. 2007;31(3):153-9. PMid:17550037.

Ehlen LA, Marshall TA, Qian F, Wefel JS, Warren JJ. Acidic beverages increase the risk of in vitro tooth erosion. Nutr Res. 2008 May;28(5):299-303. PMid:19083423.

Caneppele TMF, Jeronymo RDI, Nicoló R, Araújo MAM, Soares LES. In vitro assessment of dentin erosion after immersion in acidic beverages: surface profile analysis and energy-dispersive X-ray fluorescence spectrometry study. Braz Dent J. 2012;23(4):373-8. PMid:23207852.

Pinto SC, Bandeca MC, Silva CN, Cavassim R, Borges AH, Sampaio JE. Erosive potential of energy drinks on the dentine surface. BMC Res Notes. 2013 Feb;6(1):67. PMid:23422044.

Barbour ME, Parker DM, Allen GC, Jandt KD. Human enamel erosion in constant composition citric acid solutions as a function of degree of saturation with respect to hydroxyapatite. J Oral Rehabil. 2005 Jan;32(1):16-21. PMid:15634296.

Edwards M, Creanor SL, Foye RH, Gilmour WH. Buffering capacities of soft drinks: the potential influence on dental erosion. J Oral Rehabil. 1999 Dec;26(12):923-7. PMid:10620154.

Salas MM, Nascimento GG, Huysmans MC, Demarco FF. Estimated prevalence of erosive tooth wear in permanent teeth of children and adolescents: an epidemiological systematic review and meta-regression analysis. J Dent. 2015 Jan;43(1):42-50 [Published Online Nov 8, 2014]. PMid:25446243.

Jensdottir T, Bardow A, Holbrook P. Properties and modification of soft drinks in relation to their erosive potential in vitro. J Dent. 2005 Aug;33(7):569-75 [Published Online Feb 16, 2005]. PMid:16005796.

Owens BM. The potential effects of pH and buffering capacity on dental erosion. Gen Dent. 2007 Nov-Dec;55(6):527-31. PMid:18050578.

Zandim DL, Corrêa FOB, Sampaio JEC, Rossa C Jr. The influence of vinegars on exposure of dentinal tubules: a SEM evaluation. Braz Oral Res. 2004 Jan-Mar;18(1):63-8. PMid:15273789.

Fahl N Jr. Direct-indirect class V restorations: a novel approach for treating noncarious cervical lesions. J Esthet Restor Dent. 2015 Sep-Oct;27(5):267-84 [Published Online Jun 1, 2015]. PMid:26031691.

Rios D, Honorio HM, Magalhaes AC, Wiegand A, Machado MAAM, Buzalaf MA. Light cola drink is less erosive than the regular one: an in situ/ex vivo study. J Dent. 2009 Feb;37(2):163-6. PMid:19097679.

Amaechi BT, Higham SM, Edgar WM. Factors influencing the development of dental erosion in vitro: enamel type, temperature and exposure time. J Oral Rehabil. 1999 Aug;26(8):624-30. PMid:10447814.

Van Eygen I, Vannet BV, Wehrbein H. Influence of a soft drink with low pH on enamel surfaces: an in vitro study. Am J Orthod Dentofacial Orthop. 2005 Sep;128(3):372-7. PMid:16168334.

Shellis RP, Barbour ME, Jesani A, Lussi A. Effects of buffering properties and undissociated acid concentration on dissolution of dental enamel in relation to pH and acid type. Caries Res. 2013;47(6):601-11 [Published Online Sep 21, 2013]. PMid:24061229.

Tenuta LM, Fernández CE, Brandão AC, Cury JA. Titratable acidity of beverages influences salivary pH recovery. Braz Oral Res. 2015;29(1):1-6. PMid:25715032.

Min JH, Kwon HK, Kim BI. Prevention of dental erosion of a sports drink by nano-sized hydroxyapatite in situ study. Int J Paediatr Dent. 2015 Jan;25(1):61-9 [Published Online Mar 17, 2014]. PMid:24628844.

Cruz NV, Pessan JP, Manarelli MM, Souza MD, Delbem AC. In vitro effect of low-fluoride toothpastes containing sodium trimetaphosphate on enamel erosion. Arch Oral Biol. 2015 Sep;60(9):1231-6 [Published Online Jun 6, 2015]. PMid:26092765.

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