Revista de Odontologia da UNESP
http://revodontolunesp.com.br/article/doi/10.1590/1807-2577.0079
Revista de Odontologia da UNESP
Original Article

The biocompatibility of a new endodontic paste used in dental trauma

Biocompatibilidade de uma nova pasta utilizada em traumatismo dentário

Soares, Adriana de Jesus; Prado, Maíra do; Brazão, Mariana Amade; Gomes, Brenda Paula Figueiredo de Almeida; Zaia, Alexandre Augusto

Abstract

Objective: This study investigated the short-term subcutaneous tissue reaction of a new endodontic paste, based on the association among calcium hydroxide [Ca(OH)2], 2% chlorhexidine gel (CHX) and zinc oxide (ZnO) in comparison with a control paste, and the individual substances alone. Material and method: Twenty-four male wistar rats were used. Polyethylene tubes containing the materials were implanted into the dorsal connective tissue of rats. Empty implanted tubes served as controls. The materials used were: Ca(OH)2+CHX+ZnO paste in two different proportions (2:1:2) and (2:1:3), a control paste, Ca(OH)2+CHX (2:1), and the substances alone. After time intervals of 2, 3, 7 and 15 days, the animals were euthanized and the specimens prepared for histological processing. Data were evaluated qualitatively according to the levels of tissue repair and the degree of inflammation. Result: On the second day all the materials were observed to cause a severe inflammatory response. On the third day, the pastes Ca(OH)2+CHX+ZnO (2:1:2) and (2:1:3) showed reduction in degree of inflammation. On seventh day the tissue was already more organized with presence of collagen fibers in all materials. After 15 days it was found that compound Ca(OH)2+CHX+ZnO (2:1:2) showed areas with no inflammatory infiltrate. Conclusion: The experimental pastes, Ca(OH)2+CHX+ZnO in two different proportions (2:1:2) and (2:1:3), showed favorable results with respect to biocompatibility, when evaluated for a short-term.

Keywords

Materials testing, chlorhexidine, calcium hydroxide, zinc oxide, toxicity.

Resumo

Objetivo: O presente estudo investigou a reação em tecido subcutâneo de ratos, por um curto período, de uma nova pasta endodôntica, obtida a partir da associação entre o hidróxido de cálcio [Ca(OH)2], clorexidina gel 2% (CHX) e óxido de zinco (ZnO), em comparação à pasta controle, e as substâncias isoladamente. Material e método: Foram utilizados vinte e quatro ratos wistar machos. Tubos de polietileno contendo os materiais foram implantados no tecido conjuntivo dorsal de ratos. Tubos vazios foram utilizados como controle. Os materiais utilizados foram: Pasta de Ca(OH)2 + CHX + ZnO em duas proporções diferentes (2: 1: 2) e (2: 1: 3), pasta controle, Ca(OH)2 + CHX (2:1), e as substâncias isoladas. Após intervalos de tempo de 2, 3, 7 e 15 dias, os animais foram sacrificados e as amostras preparadas para processamento histológico. Os dados foram avaliados qualitativamente de acordo com os níveis de reparo dos tecidos e do grau de inflamação. Resultado: No segundo dia observou-se que todos os materiais causaram uma resposta inflamatória grave. No terceiro dia, as pastas de Ca(OH)2 + CHX + ZnO (2: 1: 2) e (2: 1: 3) mostraram uma redução no grau de inflamação. No sétimo dia, o tecido encontrava-se mais organizado, com a presença de fibras colágenas em todos os materiais. Após 15 dias, verificou-se que a pasta de Ca(OH)2 + CHX + ZnO (2: 1: 2) mostrou áreas sem infiltrado inflamatório. Conclusão: As pastas experimentais, Ca(OH)2 + CHX + ZnO, nas duas proporções avaliadas (2: 1: 2) e (2: 1: 3), mostraram resultados favoráveis em relação à biocompatibilidade, quando avaliada por curto período de tempo.

Palavras-chave

Teste de materiais, clorexidina, hidróxido de cálcio, óxido de zinco, toxicidade.

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