Adenocarcinoma polimorfo de baixo grau de malignidade. Estudo ultra-estrutural e imuno-histoquímico
Polymorphous low-grade adenocarcinoma. An ultrastructural and immunohistochemical study
Carvalho, Y.R.; Araújo, N.S.; Araújo, V.C.; Sesso, A.
Rev. odontol. UNESP, vol.22, n1, p.19-29, 1993
Resumo
Seis casos de adenocarcinomas de baixo grau de malignidade foram estudados por microscopia de luz e eletrônica e imuno-histoquimica. Os seguintes padrões histológicos foram identificados: papilar, sólido, tubular, pseudocístico, trabecular e raramente cribriforme. Utilizando o método da peroxidase-antiperoxidase (PAP), o filamento intermediário vimentina, queratina e proteína S100 foram observados nas células tumorais. Ao microscópio eletrônico, as células tumorais exibiam citoplasma relativamente claro e quantidade limitada de cistemas de retículo endoplasmático rugoso. Feixes de mamentos intermediários foram verificados próximo ao núcleo e em áreas citoplasmáticas mais distantes. Células com microvilos, revestindo espaços semelhantes a lumes, foram também observadas. A análise morfológica e imuno-histoquímica revelou dois tipos de células neoplásicas: mioepitelial e luminal. Células com aspectos intermediários entre estes dois tipos estavam presentes, indicando uma possível conversão de células lumínais em míoepiteliais.
Palavras-chave
Neoplasias das glãndulas salivares, adenocarcinoma
Abstract
Six cases of polymorphous low-grade adenocarcinomas were studied by light and electron microscopy and immunohistochemistry. The following patterns of histological appearance have been found: papillary, solid, tubular, pseudocystic, trabecular and rarely cribriform. Utilizing the peroxidase-antiperoxidase (PAP) method, the intermediate filament vimentin, keratin and S100 protein are observed in tumoural cells. Under the electron microscope, tumour cells have relatively clear cytoplasms and limited amounts of rough endoplasmic cisternae. Intermediate size filament bundles are observed dose to the nuclei and in more distant cytoplasmic areas. Cells with microvilli lining lumen like spaces are also seen. Morphological and immunohistochemical analysis reveal two types of neoplastic cells: myoepithelial and luminal. Cells with intermediate features between these two types are also seen, indicating a possible conversion of luminal cells into myoepithelial ones.
Keywords
Salivary gland neoplasms, adenocarcinoma
References
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