Ultra-estrutura do corpúsculo lamelar sensitivo da mucosa gengival do macaco Cebus apella
Ultrastructure of the sensitive lamellar corpuscle of the gingival mucosae of the Cebus apella
Watanabe, I-S
Rev. odontol. UNESP, vol.11, nÚnico, p.37-45, 1982
Resumo
Corpúsculos lamelares sensitivos subepiteliais da mucosa gengival de macaco Cebus apella foram estudados através da microscopia óptica e eletrônica. Foi evidenciado que o corpúsculo sensitivo localiza-se no espaço papilar do epitélio e não apresenta contactos íntimos com a lâmina basal. A morfologia é variável e apresenta-se constituída por axônios terminais dispostos em vários níveis. As expansões irregulares de axônios em formas achatadas ou discoidais apresentam-se rodeadas de lamelas citoplasmáticas e contêm grande quantidade de mitocôndrias, vesículas claras e corpos multivesiculares. O citoplasma da célula lamelar apresenta organelas bem evidentes tais como as mitocôndrias, microfilamentos, microtúbulos, retículo endoplasmático granular e caveolas. As junções do tipo desmosomos são observadas entre a membrana do axônio e a lâmina citoplasmática adjacente e entre as lamelas sucessivas.
Palavras-chave
Corpúsculo lamelar, mucosa gengival, terminação nervosa sensitiva, ultra-estrutura
Abstract
Gingival mucosae of adult Cebus apella monkey were fixed for three hours in modified Karnovsky fixative containing 2.5% glutaraldehyde, 2% formaldehyde in sodium phosfate buffer at 0.1M and pH = 7.4. The specimens were refixed in 1% osmium tetroxide in 0.1M sodium phosfate buffer ai 4ºC for two hours, dehydrated in graded alcohols and embedded in Epon 812. Thick sections of 1-3 um and ultrathin sections of 40-80 nm were obtained with glass knives on a LKB ultramicrotome. The thick seclions were stained with toluidin blue solution and the grids, also stained with uranyl acetate and lead citrate for exam in a Philips EM-301 electron microscope. The observations of our results permit to conclude: 1) the gengival mucosae of Cebus apella monkey present the lamellar nerve ending subepithelially and the lamellar corpuscle is localized in the papillar space of epithelium and does not contact with the basement membrane; so, many bundles of collagen fibers are present between them; 2) the nerve ending is constituted by an afferent fiber that subdivide several times and formes irregular flattened or discoidal expansions; 3) the cytoplasmic lamina of lamellac cells are thin near the terminal axon becoming large and irregular in shape when located ai the periphery portion of the corpuscle; 4) the cytopJasm of the lamellae cells is characlerized by the presence of numerous microfilaments, microtubules, mitochondria, caveolae, and rough endoplasmic reticulum; 5) desmosome type junctions are observed between the axoplasm membrane and the adjacenl cytoplasmic lamina and between the lamellac; 6) the terminal axon present a great quantity of mitochondria, myelin figures, clear vesicles and mutivesicular bodies.
Keywords
Corpúsculo lamelar, mucosa gengival, terminação nervosa sensitiva, ultra-estrutura
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